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Method for extracting pectin from shaddock ped by means of laccase pretreatment

A pretreatment and enzyme treatment technology, applied in the field of enzyme extraction and natural products, can solve the problem of no literature reports or patent applications for laccase application, and achieve high content of total galacturonic acid in pectin, easy operation and good quality Effect

Inactive Publication Date: 2018-05-18
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the application of laccase in pomelo peel pectin extraction has no literature report or patent application

Method used

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  • Method for extracting pectin from shaddock ped by means of laccase pretreatment
  • Method for extracting pectin from shaddock ped by means of laccase pretreatment
  • Method for extracting pectin from shaddock ped by means of laccase pretreatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Laccase pretreatment is the method for extracting pectin from pomelo peel, and concrete steps are as follows:

[0031] 1) Raw material pretreatment: Pomelo peel is crushed and passed through a 100-mesh sieve. Take 100g of pomelo peel powder and put it into a beaker, add distilled water according to the ratio of material to liquid at 1:10 and place it in a boiling water bath for heating. Stir while heating. Continue to keep warm for 10 minutes after rising to above 90°C;

[0032] 2) Laccase pretreatment: Cool the raw materials after the above heat treatment to room temperature, adjust the solution to pH 4.0 with 0.5mol / L NaOH buffer solution, add 500U laccase, and incubate in a constant temperature water bath at 40°C for 30min, during which every 5min Stir once;

[0033] 3) Acid hydrolysis treatment: After the above laccase treatment, the solution was cooled to room temperature, and the pH of the solution was adjusted to 2 with 1.0mol / L HCl, and kept in a constant tempe...

Embodiment 2

[0039] This example is the same as Example 1, except that the amount of laccase added in this example is 1000 U.

[0040] After testing, the extraction yield of the obtained pectin was 22.1%, the total galacturonic acid was 76.4%, the methoxyl content was 11.8%, the degree of esterification was 72.4%, the solubility was 94.2%, the ash content was 2.6%, hydrochloric acid The insoluble matter is 0.3%, the loss on drying is 3%, the pH is 2.7, and the product quality meets the national standard.

Embodiment 3

[0042] This example is the same as Example 1, except that the amount of laccase added in this example is 2000U.

[0043] After testing, the extraction yield of the obtained pectin is 23.5%, the total galacturonic acid is 78.7%, the methoxyl content is 11.8%, the degree of esterification is 72.4%, the solubility is 98.2%, the ash content is 2.5%, hydrochloric acid The insoluble matter is 0.3%, the loss on drying is 3%, the pH is 2.8, and the product quality meets the national standard.

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Abstract

The invention discloses a method for extracting pectin form shaddock ped by means of laccase pretreatment, and relates to a natural product processing method for adopting the shaddock ped as a raw material. According to the method, after laccase is utilized to carry out pretreatment on shaddock ped powder, the shaddock ped powder is subjected to hydrochloric acid acidolysis, active carbon decoloration, evaporation and concentration, ethanol precipitation, drying and other processes to obtain a pectin product. According to the method, by utilizing the laccase to carry out pretreatment, lignin in the shaddock ped can be effectively degraded, the surface microstructure of the shaddock ped powder can be damaged to facilitate releasing of the pectin during acidolysis, and therefore the purposeof increasing the extraction rate of the pectin is achieved. The obtained pectin has the advantages of being great in solubleness, high in esterification degree, less in ash content and the like. Themethod can increase the comprehensive utilization value of the shaddock ped, reduce pollution caused by waste shaddock ped to the environment and can also be applied to technological processes of extracting the pectin from other natural raw materials containing lignin at the same time.

Description

technical field [0001] The invention belongs to the technical field of natural products, relates to the technical field of enzyme extraction, in particular to a method for extracting pectin from pomelo peel by pretreatment with laccase. Background technique [0002] Pectin is a linear high molecular polysaccharide polymer composed of hundreds to thousands of D-galacturonic acid unit structure, which has good gelation effect. Pectin can be used as a natural functional factor to act as a stabilizer, thickener and emulsifier in food. It also has a certain physiological function of lowering blood sugar and lowering blood lipids. It is used in beverages, dairy products, jams and candies. It has a wide range of applications in the processing field, as well as in the fields of medicine and fine processing. Commercial pectin is mainly extracted from natural substances rich in pectin, such as by-products of pulp processing such as apple peel and citrus peel. [0003] Pomelo peel ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
Inventor 李松丁重阳张克明葛飞陶玉贵魏胜华
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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