Cell line stably expressing African swine fever virus p54 protein and its preparation and application

Abstract

The invention provides a Vero cell line capable of stably expressing protein P54 of an African swine fever virus. The collection number of the cell line is CGMCC No.14316. The cell line not only can identify a polyclonal antibody of African swine fever, but also can generate specific reaction with positive serum of an African swine fever-infected pig. The monoclonal Vero cell line of an African swine fever P4 antigen can be used for stably expressing the protein P54 of the African swine fever virus, and can be used as a cell model or a virus infection model; the cell transmission stability ishigh, the growing speed is high, and culture conditions are simple; a large number of cell lines can be provided; the cell line has a good application prospect for researches on detection and pathogenesis mechanisms of the African swine fever.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and immunization, in particular to a cell line stably expressing African swine fever virus P54 protein, a preparation method and application thereof. Background technique [0002] African swine fever (ASF) is a severe and highly contagious infectious disease of pigs caused by African swine fever virus (ASFV). African swine fever outbreaks were first recorded in Kenya in 1921 and are now endemic in most African countries in sub-Saharan Africa. The disease was introduced to southern Europe in 1957, to the Caribbean and South America in 1971, to the Caucasus and Russia in 2007, and is still prevalent in Africa, South Sardinia, the Caucasus and Russia. The World Organization for Animal Health (OIE) lists it as an animal disease that must be reported, and my country lists African swine fever as a first-class animal disease. One of the 13 major exotic animal diseases that are prioritized f...

AI Technical Summary

Problems solved by technology

[0005] At present, there are mainly the following main methods for the detection of this epidemic: 1) ELISA methods based on the main antigen-antibody, including ELISA kits based on P72 and P54, these kits are expensive, and foreign companies have set up patents Protection barriers, there are no mature commercial kits in China; 2) detection methods based on pathogenic nucleic acid, including PCR, fluorescent PCR, etc., but these methods have the disadvantage of easy cross-contamination, and need other methods to assist in further identification; 3) based on IFA research method of virology, which is the laboratory confirmation method recommended by OIE

However, this method has a virus infection process, the operation is cumbersome and time-consuming, and it involves biosafety issues, so the scope of application is limited, and an alternative method for this method is urgently needed

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