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Alkaline medium and application thereof

A culture medium and alkaline technology, applied in the direction of bacteria, can solve the problems of long fermentation process of lactic acid bacteria, inability to quickly enter the logarithmic phase, and inability to adapt to the environment in time, so as to promote rapid reproduction, promote proliferation and secretion of products , the effect of increasing the breeding time

Active Publication Date: 2018-04-20
南京雷德蒙德农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the beginning of fermentation, lactic acid bacteria cannot adapt to the environment in time due to the lack of various trace elements required for microbial growth in the medium, and cannot quickly enter the logarithmic phase; as the fermentation continues, metabolites such as lactic acid continue to accumulate, pH Continuously decline until the growth of lactic acid bacteria is inhibited. Due to these conditions, the entire lactic acid bacteria fermentation process takes a long time, the product is less, and the fermentation result is not ideal

Method used

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Examples

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Effect test

Embodiment 1

[0025] The alkaline medium of this embodiment is prepared by mixing medium A and medium B in a ratio of 3:1000; wherein, in parts by weight,

[0026] The preparation method of culture medium A is as follows: 9 parts of calcium aluminum silicate, 9 parts of sodium aluminum silicate, 400 parts of sodium silicate, 600 parts of potassium silicate, 530 parts of potassium carbonate, 530 parts of sodium carbonate, 1.62 parts of zinc oxide, 12 parts of sodium thiosulfate, 2.44 parts of germanium dioxide, 1350 parts of glucose, and 1000 parts of water were mixed and dissolved, heated to 90°C, maintained at a redox potential of -40 to 40mV under nitrogen flow conditions, and thoroughly mixed and stirred. After 2.5h, it became gel-like after cooling, and medium A was obtained. Among them, the medium A is light yellow gelatinous, the specific gravity is 1.42, the viscosity is 180, and the pH value is 14.0.

[0027] The preparation method of medium B is: 10 parts of peptone, 6 parts of ye...

Embodiment 2

[0029] The alkaline medium of this embodiment is prepared by mixing medium A and medium B in a ratio of 1:1000; wherein, by weight,

[0030] The preparation method of culture medium A is as follows: 7 parts of calcium aluminum silicate, 7 parts of sodium aluminum silicate, 500 parts of sodium silicate, 500 parts of potassium silicate, 1000 parts of potassium carbonate, 2.2 parts of zinc oxide, 8 parts of sodium thiosulfate parts, 3.5 parts of germanium dioxide, 700 parts of glucose, 700 parts of fructose, and 1000 parts of water, mixed and dissolved, heated to 80°C, and maintained at a redox potential of -40 to 40mV under nitrogen flow conditions, mixed well, and stirred for 2 -3h, it becomes gelatinous after cooling, and medium A is obtained. Among them, the medium A was light yellow gelatinous, the specific gravity was 1.40, the viscosity was 185, and the pH value was 14.0.

[0031] The preparation method of medium B is: 12 parts of peptone, 5 parts of yeast extract, 15 par...

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Abstract

The invention provides an alkaline medium and application thereof, which relate to the technical field of microbial fermentation. The alkaline medium is prepared by mixing a medium A and a medium B according to the proportion of (1-5):1000, and the main ingredients include aluminide, silicide, carbide, glucose, fructose, sucrose, maltose and the like. The alkaline medium disclosed by the inventioncan supplement trace elements needed in the process of bacterial fermentation, promote the rapid reproduction of bacteria, increase the growth speed of bacteria and increase product concentration.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to an alkaline medium and application thereof. Background technique [0002] The production level of microbial fermentation depends on the characteristics of the producing strain and the control of fermentation conditions. Fermentation is a very complex production process, and its quality involves many factors, such as the production performance of strains, the ratio of medium, the quality of raw materials, sterilization conditions, seed quality, fermentation conditions and process control. [0003] Lactic acid bacteria consist of a group of bacteria with the same morphological, metabolic and physiological properties, mostly Gram-positive, spore-free cocci or bacilli, and this group of bacteria can produce large amounts of lactic acid during the fermentation of available carbohydrates. Lactic acid bacteria are mostly anaerobic bacteria, mainly anaerobic respiration, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 倪同艳王强
Owner 南京雷德蒙德农业科技有限公司
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