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Rhizoma curculigins polysaccharides, a preparing method thereof and applications of the polysaccharides

A technology of polysaccharide and curcuma, applied in the field of curcuma polysaccharide and its preparation, achieves the effects of strong activity, improved extraction rate, prevention and treatment of osteoporosis

Active Publication Date: 2018-04-10
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no reports on the anti-osteoporosis and / or rheumatism activity of curculigo polysaccharides at home and abroad, and the structure and activity of homogeneous polysaccharides. The structure and activity of homogeneous polysaccharides are necessary

Method used

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  • Rhizoma curculigins polysaccharides, a preparing method thereof and applications of the polysaccharides
  • Rhizoma curculigins polysaccharides, a preparing method thereof and applications of the polysaccharides
  • Rhizoma curculigins polysaccharides, a preparing method thereof and applications of the polysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1, the preparation method of curculigo curculigo polysaccharide

[0071] S1. Cut into sections: wash the dried Curculigo curculigo, cut into small sections of 3-5 mm, and cut into sections of Curculigo curculigo;

[0072] S2. Soaking: put the cut curculigo curculiosa obtained in S1 into a container, add 10 times the volume of the cut curculigo curculigo, soak the cut curculigo curculi overnight, and filter to obtain the curculigo curculigo raw material;

[0073] S3, water extraction: extract the Curculigo curculi raw material obtained in S2 with 5 times the volume of the Curculigo curculi raw material and hot water at a temperature of 90°C, filter, repeat the extraction 3 times, collect the crude water extract, and dry the medicinal residues;

[0074] S4, graded alcohol precipitation: the crude water extract obtained in S3 is concentrated under reduced pressure and vacuum filtered to obtain a fine water extract, and ethanol is added to the fine water extract ...

Embodiment 2

[0133] Embodiment 2, Curculigoline polysaccharide COP-1 and preparation method thereof

[0134] Curculigo extract polysaccharide COP-1, after monosaccharide composition analysis, complete methylation combined with GC-MS analysis, infrared spectrum analysis and NMR analysis, it is concluded that curculigo extract polysaccharide COP-1 is composed of mannose and glucose, and its structure is:

[0135]

[0136] where m+n=10.

[0137] The preparation method of described curculigoline polysaccharide COP-1 is:

[0138] S1. Cut into sections: wash the dried Curculigo curculigo, cut into small sections of 3-5 mm, and cut into sections of Curculigo curculigo;

[0139] S2. Soaking: put the cut curculigo curculigo medicinal material obtained in S1 into a container, add 10 times the volume of the cut curculigo curculigo to soak overnight, and filter to obtain the curculigo curculigo raw material;

[0140] S3, water extraction: extract the Curculigo curculi raw material obtained in S2 ...

Embodiment 3

[0145] Embodiment 3, curculigoline polysaccharide COP-2 and preparation method thereof

[0146] Curculigo extract polysaccharide COP-2, after monosaccharide composition analysis, complete methylation combined with GC-MS analysis, infrared spectrum analysis and NMR analysis, it is concluded that curculigo extract polysaccharide COP-2 is composed of mannose, glucose, rhamnose, Composed of arabinose, galactose, galacturonic acid and glucuronic acid, the structure is:

[0147]

[0148] where n(v+w+x+y+z+m+19)=314.

[0149] The preparation method of described curculigoline polysaccharide COP-2 is:

[0150] S1. Cut into sections: wash the dried Curculigo curculigo, cut into small sections of 3-5 mm, and cut into sections of Curculigo curculigo;

[0151] S2. Soaking: put the cut curculigo curculiosa obtained in S1 into a container, add 10 times the volume of the cut curculigo curculigo, soak the cut curculigo curculi overnight, and filter to obtain the curculigo curculigo raw ma...

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Abstract

The invention belongs to the technical field of rhizoma curculigins processing, and particularly relates to rhizoma curculigins polysaccharides, a preparing method thereof and applications of the polysaccharides. The method includes water extraction, graded alcohol precipitation, alkali extraction and alcohol precipitation, deproteinization, dialysis, freeze-drying, ion exchange column chromatography, gel (molecular sieve) column chromatography, and other steps. The three purified rhizoma curculigins polysaccharides which are COP-1, COP-2 and COP-3 can be obtained by the method. Chemical structures of the obtained three purified rhizoma curculigins polysaccharides are identified, the structures are determined, and structural basis for pharmacological activity mechanism researching is provided. In addition, the invention also provides the applications of the rhizoma curculigins polysaccharides in preparation of medicines treating osteoporosis and / or rheumatism and related diseases, healthcare products or functional foods.

Description

technical field [0001] The invention belongs to the technical field of curculigo processing, and in particular relates to a curculigo polysaccharide and its preparation method and application. Background technique [0002] Osteoporosis (OP) is a systemic bone metabolic disease characterized by decreased bone mineral mass, damaged bone microarchitecture, and decreased bone strength, resulting in increased bone fragility and susceptibility to fracture. High-risk groups are mostly the elderly and postmenopausal women. Imbalance of bone metabolism in the human body, activation of osteoclasts, enhanced bone resorption activity, increased bone loss, inhibited osteoblast function, decreased osteogenesis activity, or insufficient bone mass formation can all lead to OP. At present, drugs for the prevention and treatment of OP can be roughly divided into drugs that inhibit bone resorption (such as estrogen, bisphosphonates, etc.), drugs that promote bone formation (such as fluoride, ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P19/10A61P19/08A61P29/00A23L33/125
CPCA23L33/125A23V2002/00A61K31/715C08B37/0003C08B37/006A23V2200/306A23V2200/30A23V2250/51A23V2250/21A23V2300/14
Inventor 严春艳王雪倩
Owner GUANGDONG PHARMA UNIV
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