Mesoporous silica-lactobionic acid targeted nanoparticles loaded with Sorafenib/siRNA
A mesoporous silica, lacturonic acid target technology, applied in the direction of active ingredients of heterocyclic compounds, medical preparations of non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem that the surface of tumor cells cannot be highly enriched problems such as low concentration, low biocompatibility, and lack of targeting molecules, to achieve the effect of improving anti-tumor effect, simple preparation method, and enhanced stability
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Embodiment 1
[0033] Example 1 Synthesis of Mesoporous Silica Nanoparticles
[0034] Add 2 g of cetyltriethylammonium bromide, 0.1 g of triethanolamine and 20 mL of deionized water into the reaction flask, and react at 95°C for 1 h; Add 1.5 mL of ethyl ester, and continue to react at 95°C for 1 h after the addition; after the reaction, centrifuge at 12,000 rpm for 15 min at room temperature to obtain crude mesoporous silica nanomaterials, which are washed twice with deionized water and absolute ethanol , suspended the resulting solid in acidic ethanol (concentrated hydrochloric acid: absolute ethanol = 5:1, V:V), refluxed for 24 h to remove unreacted cetyltriethylammonium bromide, centrifuged, and deionized Washed with water and freeze-dried at -50 °C to obtain pure mesoporous silica nanoparticles.
[0035] figure 1 TEM image of the as-prepared mesoporous silica nanoparticles. It can be seen from the figure that the particle size is about 100 nm.
Embodiment 2
[0036] Example 2 Mesoporous silica nanoparticles (MSN-NH 2 ) preparation
[0037] Dissolve 100 mg of the mesoporous silica nanoparticles prepared in Example 1 in 20 mL of absolute ethanol, add 400 μg of 3-aminopropyltriethoxysilane, stir at room temperature for 12 h, centrifuge, and wash with ethanol several times to Remove unreacted 3-aminopropyltriethoxysilane, and freeze-dry to obtain surface amino-modified mesoporous silica nanoparticles (MSN-NH 2 ).
Embodiment 3
[0038] Example 3 Preparation of Sorafenib-loaded Mesoporous Silica Nanoparticles (SO@MSN)
[0039] Get 30 mg of MSN-NH prepared in Example 2 2 Dissolve in 30 mL of acetone, stir at room temperature for 1 h, add 20 mg of Sorafenib, stir for 30 min, then centrifuge at 13,000 rpm for 30 min, discard the supernatant, wash the precipitate with deionized water, freeze-dry, Sorafenib-loaded mesoporous silica nanoparticles (SO@MSN) were obtained.
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