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Method for preparing corticosterone electrochemiluminescence sensors on basis of CdTe-Ag2Se nano-composites and application of corticosterone electrochemiluminescence sensors

A nanocomposite, cdte-ag2se-ab technology, applied in the field of nanomaterials and electrochemical analysis, can solve the problems of poor reproducibility of enzyme-linked immunoassay methods, difficult to exclude cross-reactions, and high requirements for sample purification, and achieve signal response. Wide range, short response time, and improved specificity

Active Publication Date: 2017-10-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chromatography has high sensitivity and good reproducibility, but it has high requirements for sample purification, large sample demand and expensive equipment
Although ELISA and RIA have high sensitivity and simple sample pretreatment, the measurement cycle is long, and it is difficult to rule out cross-reactions caused by structural analogs in hormones
In addition, the reproducibility of enzyme-linked immunoassay method is poor, and radioimmunoassay also has problems such as radioactive waste disposal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Based on CdTe-Ag 2 Preparation method of corticosterone electrochemiluminescent sensor of Se nanocomposite

[0038] (1) Antibody capture substrate material CdTe-Ag 2 Preparation of Se-Ab solution

[0039] 1) Mesoporous silver selenide Ag 2 Preparation of Se

[0040] Add 5 mL of octadecylamine into a 50 mL beaker, heat to 150 ºC, add 0.3 g of silver nitrate, and stir for 5 min, the color of the solution changes from colorless to bright yellow, and finally dark brown; continue to add 0.1 g of selenium powder, the solution instantly changed from dark brown to black, stirred for 10 min, centrifuged, washed 3 times with ethanol to obtain the product Ag 2 Se nanoparticles; the product Ag 2 Se nanoparticles were dispersed into 20 mL non-polar solvent cyclohexane to prepare Ag 2 Se nanoparticle cyclohexane solution;

[0041] 5 mL of Ag 2 Se nanoparticle cyclohexane solution, 0.2 g cetyltrimethylammonium bromide and 0.2 g sodium dodecylbenzenesulfonate were added to 8...

Embodiment 2

[0052] 1. Based on CdTe-Ag 2 Preparation method of corticosterone electrochemiluminescent sensor of Se nanocomposite

[0053] (1) Antibody capture substrate material CdTe-Ag 2 Preparation of Se-Ab solution

[0054] 1) Mesoporous silver selenide Ag 2 Preparation of Se

[0055] Add 15 mL of octadecylamine into a 50 mL beaker, heat to 200 ºC, add 0.7 g of silver nitrate, and stir with magnetic force for 15 min, the color of the solution changes from colorless to bright yellow, and finally dark brown; continue to add 0.15 g of selenium powder, the solution instantly changed from dark brown to black, stirred for 10 min, centrifuged, washed 3 times with ethanol, and the product Ag 2 Se nanoparticles; the product Ag 2 Se nanoparticles were dispersed into 20 mL non-polar solvent cyclohexane to prepare Ag 2 Se nanoparticle cyclohexane solution;

[0056] 15 mL of Ag 2 Se nanoparticles cyclohexane solution, 0.7 g cetyltrimethylammonium bromide and 0.6 g sodium dodecylbenzenesulfo...

Embodiment 3

[0067] 1. Based on CdTe-Ag 2 Preparation method of corticosterone electrochemiluminescent sensor of Se nanocomposite

[0068] (1) Antibody capture substrate material CdTe-Ag 2 Preparation of Se-Ab solution

[0069] 1) Mesoporous silver selenide Ag 2 Preparation of Se

[0070] Add 10 mL of octadecylamine into a 50 mL beaker, heat to 170 ºC, add 0.5 g of silver nitrate, and stir with magnetic force for 10 min, the color of the solution changes from colorless to bright yellow, and finally dark brown; continue to add 0.12 g of selenium powder, the solution instantly changed from dark brown to black, stirred for 10 min, centrifuged, washed 3 times with ethanol, and the product Ag 2 Se nanoparticles; the product Ag 2 Se nanoparticles were dispersed into 20 mL non-polar solvent cyclohexane to prepare Ag 2 Se nanoparticle cyclohexane solution;

[0071] 8 mL of Ag 2 Se nanoparticle cyclohexane solution, 0.5 g cetyltrimethylammonium bromide and 0.4 g sodium dodecylbenzenesulfona...

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Abstract

The invention relates to a method for preparing corticosterone electrochemiluminescence sensors on the basis of CdTe-Ag2Se nano-composites and application of the corticosterone electrochemiluminescence sensors, and belongs to the technical field of nano-materials and electrochemical analysis. The corticosterone electrochemiluminescence sensors are prepared from the CdTe-Ag2Se nano-composites with mesoporous silver selenide loaded cadmium telluride quantum dots, and the CdTe-Ag2Se nano-composites are used as corticosterone capture antibody substrate materials. The method and the application have the advantage that corticosterone can be sensitively detected.

Description

technical field [0001] The present invention relates to a CdTe-Ag based 2 Preparation and application of Se nanocomposite-based corticosterone electrochemiluminescent sensors. It specifically involves cadmium telluride quantum dots CdTe QDs as electrochemiluminescent materials, using mesoporous silver selenide Ag with the advantages of high porosity, large specific surface area, non-toxicity, and good biocompatibility. 2 Se is used as a carrier material to increase the immobilization capacity of luminescent materials and antibodies, and to prepare a label-free electrochemiluminescent sensor for detecting corticosterone, which belongs to the field of nanomaterials and electrochemical analysis. Background technique [0002] Corticosterone (corticosterone) is the most important glucocorticoid secreted by the adrenal cortex in the human body, and plays an important role in the metabolism of sugar, protein, fat and various organ functions necessary for maintaining life. Insuffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N21/76
CPCG01N21/76G01N27/3277G01N27/3278
Inventor 朱文娟魏琴王斌鞠熀先范大伟张勇吴丹
Owner UNIV OF JINAN
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