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Bovine viral diarrhea-bovine infectious rhinotracheitis bivalent subunit vaccine and preparation method and application thereof

A technology for bovine viral diarrhea and rhinotracheitis, applied in biochemical equipment and methods, vaccines, viruses, etc., can solve the problems of poor immune effect of vaccines, hidden dangers of biological safety, virus mutation, etc., and achieve no immune interference, immune Strong originality and cost-saving effect

Pending Publication Date: 2017-09-19
NOVO BIOTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problems to be solved by the present invention: one is to provide a bovine viral diarrhea-cattle virus with strong immunogenicity, good safety, no immune interference, ability to distinguish wild virus infection and vaccine immunity, and can fundamentally purify BVDV and IBRV The dual subunit vaccine of infectious rhinotracheitis achieves the purpose of preventing bovine viral diarrhea and bovine infectious rhinotracheitis at the same time; the second is to overcome the possibility of BVDV or IBRV and other virus genomes or Residual nucleic acid recombination in other vaccines causes virus mutations, resulting in great biological safety hazards and possible immune interference problems; the third is to overcome the possibility that inactivated dual vaccines may have nucleic acid molecules that have not been completely inactivated. Molecules may recombine with wild-type BVDV or IBRV, leading to further mutation of the virus, resulting in the risk of poor vaccine immunity; the fourth is to provide a dual subunit vaccine for the preparation of bovine viral diarrhea-bovine infectious rhinotracheitis Method and application in prevention and treatment of bovine viral diarrhea and bovine infectious rhinotracheitis

Method used

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  • Bovine viral diarrhea-bovine infectious rhinotracheitis bivalent subunit vaccine and preparation method and application thereof
  • Bovine viral diarrhea-bovine infectious rhinotracheitis bivalent subunit vaccine and preparation method and application thereof
  • Bovine viral diarrhea-bovine infectious rhinotracheitis bivalent subunit vaccine and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of bovine viral diarrhea virus E2 protein and bovine infectious rhinotracheitis virus gD protein

[0029] 1.1 Refer to the patent application document of the Chinese invention patent application publication number CN 105924506 A or the invention patent application document of the company with the application number 201611208261.8 or other protein expression methods (such as prokaryotic expression system expression) or bovine virus in other patents or literature The preparation method of bovine viral diarrhea virus E2 protein prepares the bovine viral diarrhea virus E2 protein.

[0030] 1.2 Refer to the application number 201610225968.3 for invention patent application documents or other protein expression methods (such as prokaryotic expression system expression, insect baculovirus expression, CHO cell expression system, etc.) or bovine infectious rhinotracheitis virus gD protein in other patents or literature The preparation method prepare...

Embodiment 2

[0031] Embodiment 2: the preparation of bovine viral diarrhea-bovine infectious rhinotracheitis dual subunit vaccine (illustrate with the preparation of 2ml / head part, 200ml altogether)

[0032] The consumables and materials used for the preparation of vaccines need to undergo aseptic treatment in advance, and the preparation process is completed in a biological safety cabinet or other instruments or environments that can ensure that the entire preparation process is sterile.

[0033] 1. Preparation of ISA 201 VG: According to the volume ratio of antigen solution and adjuvant of 46:54, measure 108ml of adjuvant into a pre-prepared reagent bottle, seal it, and preheat it in a 33°C water bath for about 30 minutes.

[0034] 2. According to the volume ratio of antigen solution and adjuvant is 46:54, the total volume of aqueous phase is 92ml. According to the protein concentration of bovine viral diarrhea virus E2 (BVDV-E2) and the protein concentration of bovine infectious rhinotr...

Embodiment 3

[0040] Embodiment 3: The immunization experiment of bovine viral diarrhea-bovine infectious rhinotracheitis dual subunit vaccine

[0041] 3.1 Vaccine preparation

[0042] Proteins and vaccines were prepared according to the methods of Examples 1 and 2, and the specific vaccine information is shown in Table 2 below:

[0043] Table 2

[0044]

[0045] 3.2 Immunization experiment

[0046] Screen 40 calves aged 4-5 months (negative for BVDV and IBRV antigen antibodies), and randomly divide them into 8 groups, 5 calves in each group. One group is used as the blank control group, and the other 7 groups are used as the immunization group, and are immunized with vaccines 1 to 7 respectively. . The blank control group was injected intramuscularly with 2ml of normal saline each time, and the other 6 groups of immunized groups were injected intramuscularly with 2ml of the corresponding vaccine each time. Three weeks after the initial immunization, a booster immunization was given o...

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Abstract

The invention discloses a bovine viral diarrhea-bovine infectious rhinotracheitis bivalent subunit vaccine and a preparation method and application thereof, and belongs to the technical field of animal vaccines and animal biological products. The vaccine comprises bovine viral diarrhea virus E2 protein, bovine infectious rhinotracheitis virus gD protein and pharmaceutically acceptable adjuvant. The preparation method for the vaccine comprises the following steps that: 1) preparing the bovine viral diarrhea virus E2 protein and the bovine infectious rhinotracheitis virus gD protein; 2) mixing the bovine viral diarrhea virus E2 protein and the bovine infectious rhinotracheitis virus gD protein prepared in the 1) to prepare antigen liquid; 3) carrying out mixing emulsion on the anti-agent liquid with ISA 201VG at an volume ratio of 46:54. The vaccine has the advantages of high immunogenicity, high safety and no immunity interference; in addition, cows can be effectively prevented and protected from the infection of the bovine viral diarrhea virus and the bovine infectious rhinotracheitis virus, an effect of two prevention functions by one injection can be achieved, time and labor are saved, and cost is saved.

Description

technical field [0001] The invention relates to a preparation method and application of a bovine viral diarrhea-bovine infectious rhinotracheitis dual subunit vaccine, belonging to the technical field of animal vaccines and veterinary biological products. Background technique [0002] Bovine viral diarrhea (BVD), also known as mucosal diaease (MD), is caused by viral diarrhea virus (BVDV), and its clinical manifestations are diarrhea, high fever, reproductive impairment, immune A disease characterized by suppression, persistent infection, and severe mucosal inflammation, erosion, and necrosis. BVDV belongs to the Pestivirus genus of the Flaviviridae family. The disease was first discovered in the United States in 1946, and then became an explosive global epidemic. It is widely spread in the United States, Britain, Germany, Switzerland, Australia, India, Japan and other large cattle-raising countries. In 1983, the disease was reported for the first time in my country, and ...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/15A61K39/265A61P31/14A61P1/12A61P31/22A61P11/00A61P11/02
CPCA61K39/12A61K39/39A61K2039/552A61K2039/70C12N2710/16034C12N2770/24334
Inventor 钱泓吴有强卞广林张强查银河
Owner NOVO BIOTECH CORP
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