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Culture medium capable of promoting growth of mycelia of morchella esculenta

A technology for mycelium growth and morel fungus, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of insufficient supply of morel mycelium, and achieve low price, accelerated growth rate, and wide-ranging sources Effect

Inactive Publication Date: 2017-09-08
张云辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defect of existing culture medium formula, provide a kind of method that can promote the growth of hickory chick hyphae, to solve the defect of insufficient supply of hickory chick mycelium, improve the output of hickory chick polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Optimization test of carbon source and nitrogen source

[0015] Carbon source: add 2% glucose, fructose, maltose, sucrose, soluble starch, corn flour, and flour to the basal medium as carbon sources to form seven different carbon source culture media, and use the basal medium as a carbon-free source The control group was used to study the effects of different carbon sources on the growth of Morchella mycelium.

[0016] Studies have found that maltose is the most suitable carbon source for morel bacteria. The effect of maltose as carbon source is the best, the mycelial growth rate is 2,89mm / d, the mycelial diameter is 42.59mm, and the mycelial growth index is 13.50. From the analysis of mycelial growth index, the colony diameter of maltose was significantly increased compared with the control group.

[0017] Nitrogen source: Add 0.3% peptone, yeast powder, soybean powder, ammonium tartrate, ammonium sulfate, and ammonium nitrate as nitrogen sources to the bas...

Embodiment 2

[0020] A medium for promoting the growth of hickory chick hyphae, the formula of which is as follows: maltose 16g / L; ammonium tartrate 0.25g / L; KH2PO4 0.8g / L; MgSO4•7H2O 0.3g / L; VB1 8mg / L; Agar 18g / L.

[0021] The configuration method is: weigh maltose, ammonium tartrate, KH2PO4, MgSO4•7H2O, VB1, dissolve in 1L of water and add, stir well, add agar and heat and stir until the agar melts; put it into a triangular flask, and use Seal with parafilm and sterilize at 121°C for 30 minutes for later use.

[0022] Morel culture:

[0023] (1) Inoculation: When the temperature of the culture medium drops to 36°C, inoculate morels, seal them with a parafilm and place them in an incubator for cultivation;

[0024] (2) Mycelia growth: Morchella was grown on the above-mentioned medium for 10 days in total, and the line was drawn on the third day after inoculation, and then lined every 24 hours for a total of 6 times; at the same time, observe the color and thickness of the mycelia , dens...

Embodiment 3

[0026] A culture medium for promoting the growth of morel mycelium, the formula of which is as follows: maltose 18g / L; ammonium tartrate 0.28g / L; KH2PO4 1.0g / L; MgSO4•7H2O 0.4g / L; VB110mg / L; agar 20gg / L.

[0027] The configuration method is: weigh maltose, ammonium tartrate, KH2PO4, MgSO4•7H2O, VB1, dissolve in 1L of water and add, stir well, add agar and heat and stir until the agar melts; put it into a triangular flask, and use Seal with parafilm and sterilize at 121°C for 30 minutes for later use.

[0028] Morel culture:

[0029] (1) Inoculation: When the temperature of the culture medium drops to 38°C, inoculate morels, seal them with a parafilm and place them in an incubator for cultivation;

[0030] (2) Mycelia growth: Morchella was grown on the above-mentioned medium for 10 days in total, and the line was drawn on the third day after inoculation, and then lined every 24 hours for a total of 6 times; at the same time, observe the color and thickness of the mycelia , d...

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Abstract

The invention provides a culture medium capable of promoting growth of mycelia of morchella esculenta. According to the culture medium, maltose serves as an optimal carbon source for mycelium culture of the morchella esculenta, ammonium tartrate serves as an optimal nitrogen source, and accurate-ingredient high-purity chemical reagents are selected to carry out preparing, so that the accuracy of ingredients of the culture medium is guaranteed, and the quality control on inocula is facilitated. Optimization is carried out aiming at nutritional ingredients required during the growth of the mycelia of the morchella esculenta, the growth speed of the mycelia of the morchella esculenta is accelerated, the mycelia of the morchella esculenta can overgrow bevel surfaces 7 to 9 days after inoculation, and the mycelium growth speed is increased by about 20% compared with that of a PDA culture medium. The time for growth of the mycelia of the morchella esculenta is shortened, and the culture medium provided by the invention is simple in formula, wide in source of raw materials and low in cost, so that the production cost of the morchella esculenta can be reduced, and a reliable technical support is provided for subsequent application of the morchella esculenta.

Description

technical field [0001] The invention relates to the field of cultivation of edible and medicinal fungi, in particular to a method for promoting the growth of hickory chick hyphae by optimizing the culture medium. Background technique [0002] Morchella is a precious perennial large medicinal fungus, which gets its name because it mostly grows on Morus plants and its fruiting bodies are yellowish brown. The medicinal effect of hickory chick was first seen in "Compendium of Materia Medica" and "On the Properties of Medicine" to record that hickory chick tastes bitter and cold in nature. In traditional Chinese medicine in my country, it is used to treat symptoms such as dysentery, night sweats, metrorrhagia, and prolapse of the anus. The latest research shows that Morchella also has a unique anti-cancer effect, and is currently a medicinal fungus with the highest efficiency among internationally recognized biological cancer treatment agents. The main pharmacological active in...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张云辉
Owner 张云辉
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