Biological compound enzyme and method thereof used for straw papermaking
A compound enzymatic and biological technology, which is applied in pulp beating/refining method, papermaking, using microorganism/enzymatic cellulose treatment, etc.
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Embodiment 1
[0041] The biological compound enzyme biochemical agent is composed of the following raw materials mixed by weight percentage: white rot fungus 15%, chrysosporium flat grass fungus 20%, xylanase 20%, glucoamylase 15%, and yeast 30%.
[0042] The biological complex enzyme biochemical agent and the carrier are mixed in a mass ratio of 1:0.8.
[0043] The carrier is obtained by mixing polylactic acid-glycolic acid copolymer and chitin. The polylactic acid-glycolic acid copolymer is 20 wt% and the chitin is 80 wt%. The poly(lactic-co-glycolic acid) (PLGA) is formed by random polymerization of two monomers-lactic acid and glycolic acid, and the polymer is composed of 75% lactic acid and 25% glycolic acid , The molecular weight is 15000-25000, and its glass transition temperature is between 40-60 °C. The chitin, whose chemical name is β-(1,4)-2-acetamido-2-deoxy-D-glucose, was purchased from Qingdao Belt Biotechnology Co., Ltd.
[0044] The preparation method of the biological composite ...
Embodiment 2
[0047] Others are the same as Example 1, the difference is:
[0048] The biological compound enzyme biochemical agent is composed of the following raw materials mixed by weight percentage: white rot fungus 15%, chrysosporium flat grass fungus 25%, xylanase 10%, glucoamylase 20%, and yeast 35%.
[0049] The biological complex enzyme biochemical agent and the carrier are mixed in a mass ratio of 1:1. The carrier is obtained by mixing polylactic acid-glycolic acid copolymer and chitin. The polylactic acid-glycolic acid copolymer is 25wt% and the chitin is 75wt%.
[0050] The preparation method of the biological composite enzyme biochemical agent preparation includes the following steps:
[0051] The above-mentioned bacteria and enzymes are mixed according to the percentages and mixed with the carrier, stirred uniformly, and then allowed to stand for 5 hours, and finally dried at a low temperature at 6°C, and the moisture content after drying is controlled at 5% to obtain a biological com...
Embodiment 3
[0053] Others are the same as Example 1, the difference is:
[0054] The biological compound enzyme biochemical agent is composed of the following raw materials mixed by weight percentage: white rot fungus 20%, chrysosporium flat grass fungus 10%, xylanase 15%, glucoamylase 10-25%, yeast 20% -35%.
[0055] The biological complex enzyme biochemical agent and the carrier are mixed in a mass ratio of 1:1.2. The carrier is obtained by mixing polylactic acid-glycolic acid copolymer and chitin. The polylactic acid-glycolic acid copolymer is 30 wt% and the chitin is 70 wt%.
[0056] The preparation method of the biological composite enzyme biochemical agent preparation includes the following steps:
[0057] The above-mentioned bacteria and enzymes are mixed according to the percentage and mixed with the carrier, stirred evenly, and then allowed to stand for 8 hours, and finally placed at 2°C for low-temperature drying, and the moisture content after drying is controlled at 10% to obtain the...
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