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A kind of corneal stroma, preparation method and application

A technology of corneal stroma and cornea, applied in medical science, tissue regeneration, prostheses, etc., can solve problems such as failure to meet clinical requirements, inability to resist mechanical traction of intraocular pressure, poor collagen toughness, etc., and achieve dense matrix tissue , restore elasticity, and reduce corneal thickness

Active Publication Date: 2020-01-21
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various attempts have been made in the study of tissue engineered corneas, such as culturing bovine corneal cells on collagen, using amnion to cultivate autologous corneal limbal epithelial cells to successfully repair ocular surface damage, and using temperature-sensitive medium to cultivate autologous oral mucosa cells in vitro to repair eyes. Table. The use of autologous limbal stem cells to culture corneal epithelial cell membranes in vitro, etc. These studies have laid a theoretical foundation for the study of tissue engineering corneas, but they still cannot meet the clinical requirements, such as poor toughness of collagen, which cannot resist intraocular pressure and sutures. The mechanism of corneal wound healing still needs to be further studied

Method used

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  • A kind of corneal stroma, preparation method and application
  • A kind of corneal stroma, preparation method and application
  • A kind of corneal stroma, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation method of a kind of corneal stroma of the present embodiment comprises the following steps:

[0048] (1) Raw material handling:

[0049] The eyeballs of the pigs within 4 hours of death were taken, the blood was rinsed with normal saline, the cornea was drilled with a trephine with a diameter of 8.5mm, rinsed with purified water, and then soaked in purified water.

[0050] (2) freeze-thaw swelling:

[0051] Soak the cornea with a small amount of water, freeze it in a -40°C freezer for 1 hour, take it out and rinse it with purified water for 15 minutes after it is completely thawed, then soak it in sufficient purified water for 30 minutes, repeat the freezing and thawing process 3 times, and obtain a cornea that absorbs water and swells.

[0052] (3) Virus inactivation:

[0053] Virus inactivation is performed by immersing the cornea in a peracetic acid-ethanol solution, which can be performed in a stainless steel barrel. The concentration (percentage ...

Embodiment 2

[0072] The sample in embodiment 1 is observed by a scanning electron microscope, as attached figure 1 and 4 As shown, it can be observed that after the immunogen removal treatment, the Bowman's membrane of the cornea after immunogen removal is intact, and is not affected or damaged by the enzyme solution, which is conducive to the migration, adhesion and proliferation of corneal epithelial cells, and is conducive to the rapid and complete cornea. Epithelialization to avoid infection. If the epithelium can grow normally and cover the surface of the corneal stroma, it can effectively isolate the degradation of its collagen components by various hydrolytic enzymes, prolong the degradation time, and facilitate the reconstruction of the corneal stroma.

[0073] After removing the pre-elastic layer figure 2 and 3 As shown, it can be seen that the inside of the cornea presents a three-dimensional network structure, and the three-dimensional network structure forms a biological sc...

Embodiment 3

[0076] The sample obtained by the preparation method of the present invention is tested for physical properties, as follows:

[0077] a. Suture tensile strength: Prepare 5 samples according to Example 1, use a 3-0 non-absorbable suture at the edge of one end of the patch at 2 mm, fix the suture and the other end of the patch on the tension meter, and use 20mm Stretch at a speed of 1 / min until the suture point is torn, and record the maximum force value. The results show that the average value of the maximum value can reach 3.2N.

[0078] b. Light transmittance: 5 samples were prepared according to Example 1, and the light transmittance of air-dried samples, swollen samples, and glycerol dehydrated samples were measured respectively. Air-dried sample preparation: paste the swollen sample directly on the inner wall of the light-transmitting side of the cuvette, and send it to a blast oven to air-dry for 24 hours; preparation of the swollen sample: paste the swollen sample direct...

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Abstract

The invention provides a cornea matrix, a preparation method and an application. An animal cornea is used as a raw material; a cornea epithelial layer and cell components and DNA components in the cornea matrix are removed; a lamina elastica anterior and a local thick cornea matrix are reserved; and complete extracellular matrixes are reserved. The cornea matrix is suitable for covering of a pathological cornea, especially is suitable for patients suffering from unperforated corneal ulcer untreatable with drugs and infectious keratitis requiring interlayer transplantation therapy, and is suitable for temporary coating of corneal perforation.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a corneal stroma, a preparation method and an application. Background technique [0002] The cornea is located at the front of the eyeball and is in direct contact with the external environment. It is vulnerable to damage and infection. If the eyes are not hygienic, it is easy to cause inflammation or even cloudiness of the cornea, leading to vision loss or even blindness. According to the World Health Organization, corneal disease is the second leading cause of vision loss after cataracts. At present, the only effective means of treating corneal blindness is corneal transplantation. There are three main sources of corneas used for corneal transplantation: allogeneic cornea donation, artificial cornea and tissue engineered cornea. The limited sources of donated corneas and the serious shortage of corneal donors cannot meet the needs of many blind patients with corne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/3683A61L27/3687A61L27/3691A61L27/50A61L2430/16A61L2430/40
Inventor 赵博夏磊磊王洪权赵延瑞李学军张晋辉
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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