Cornea matrix, preparation method and application

A technology of corneal stroma and cornea, applied in medical science, tissue regeneration, prostheses, etc., can solve problems such as inability to resist mechanical traction of intraocular pressure, poor collagen toughness, and inability to meet clinical use requirements, etc., to achieve dense matrix tissue , Reduce corneal thickness and restore elasticity

Active Publication Date: 2017-08-18
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various attempts have been made in the study of tissue engineered corneas, such as culturing bovine corneal cells on collagen, using amnion to cultivate autologous corneal limbal epithelial cells to successfully repair ocular surface damage, and using temperature-sensitive medium to cultivate autologous oral mucosa cells in vitro to repair eyes. Table. The use of autologous limbal stem cells to culture corneal epithelial cell membranes in vitro, etc. These studies have laid a theoretical foundation for the study of tissue engineering corneas, but they still cannot meet the clinical requirements, such as poor toughness of collagen, which cannot resist intraocular pressure and sutures. The mechanism of corneal wound healing still needs to be further studied

Method used

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  • Cornea matrix, preparation method and application
  • Cornea matrix, preparation method and application
  • Cornea matrix, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The method for preparing corneal stroma in this embodiment includes the following steps:

[0049] (1) Raw material processing:

[0050] Take the eyeballs of pigs or cows within 4 hours of death, rinse them with normal saline, use a trephine with a diameter of 8.5mm to drill the cornea, rinse with purified water and soak in purified water.

[0051] (2) Freeze-thaw swelling:

[0052] The cornea was soaked in a small amount of water and placed in a -40°C freezer for 1 hour. After being removed, it was completely thawed and rinsed with purified water for 15 minutes, and then soaked in sufficient purified water for 30 minutes. The freezing and thawing process was repeated 3 times to obtain a water-absorbing swelled cornea.

[0053] (3) Virus inactivation:

[0054] The cornea is soaked in a peracetic acid-ethanol solution for virus inactivation. This process can be carried out in a stainless steel barrel. The concentration (volume percentage) of peracetic acid in the peracetic acid-eth...

Embodiment 2

[0073] Observe the sample in Example 1 by scanning electron microscope, as attached figure 1 with 4 As shown, it can be observed that after immunogen removal treatment, the front elastic layer of the cornea after immunogen removal is intact without being affected or damaged by the enzyme solution, which is beneficial to the migration, adhesion and proliferation of corneal epithelial cells, and is beneficial to the rapid and complete cornea Epithelialization to avoid infection. The epithelium can grow normally and cover the surface of the corneal stroma, which can effectively isolate the degradation of its collagen components by various hydrolytic enzymes, prolong the degradation time, and facilitate the reconstruction of the corneal stroma.

[0074] As attached after removing the front elastic layer figure 2 with 3 As shown, it can be seen that there is a three-dimensional network structure inside the cornea. The three-dimensional network structure forms a biological scaffold, wh...

Embodiment 3

[0077] The physical properties of the samples obtained by the preparation method of the present invention are tested as follows:

[0078] a. Suture tensile strength: Prepare 5 samples in accordance with Example 1. Use 3-0 non-absorbent sutures at 2 mm from the edge of one end of the patch, and fix the suture and the other end of the patch on the tensile tester at 20 mm Stretching at a speed of 1 / min until the suture point is torn. Record the maximum force value. The result shows that the maximum average value can reach 3.2N.

[0079] b. Light transmittance: Prepare 5 samples according to Example 1, and measure the light transmittance of the air-dried sample, the swollen sample, and the glycerin dehydrated sample. Air-dried sample preparation: directly stick the swollen sample to the inner wall of the light-transmitting side of the cuvette, and send it into a blast oven for air drying for 24 hours; swelling sample preparation: directly stick the swollen sample to the inner wall of t...

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Abstract

The invention provides a cornea matrix, a preparation method and an application. An animal cornea is used as a raw material; a cornea epithelial layer and cell components and DNA components in the cornea matrix are removed; a lamina elastica anterior and a local thick cornea matrix are reserved; and complete extracellular matrixes are reserved. The cornea matrix is suitable for covering of a pathological cornea, especially is suitable for patients suffering from unperforated corneal ulcer untreatable with drugs and infectious keratitis requiring interlayer transplantation therapy, and is suitable for temporary coating of corneal perforation.

Description

Technical field [0001] The invention relates to the technical field of medical biological materials, in particular to a corneal stroma, a preparation method and an application. Background technique [0002] The cornea is located at the forefront of the eyeball. It is in direct contact with the external environment and is susceptible to injury and infection. If the eye is not hygienic, it can easily cause corneal inflammation or even turbidity, resulting in vision loss or even blindness. According to the World Health Organization report, corneal disease is the second leading cause of vision loss, second only to cataracts. At present, the only effective treatment for corneal blindness is corneal transplantation. The cornea used in corneal transplantation mainly comes from three sources: allogeneic corneal donation, artificial cornea and tissue engineered cornea. The source of donated corneas is limited, and there is a severe shortage of corneal donors, which cannot meet the treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/3683A61L27/3687A61L27/3691A61L27/50A61L2430/16A61L2430/40
Inventor 赵博夏磊磊王洪权赵延瑞李学军张晋辉
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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