Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method and application of Kluyveromyces marx freeze-dried microbial agent

A technology of yeast and dry bacteria, which is applied in the field of preparation of Kluyveromyces marx freeze-dried bacteria, which can solve the problems of tolerance difference and achieve the effects of long storage time, simple ingredients and convenient use

Active Publication Date: 2020-04-28
XINJIANG TIANRUN BIOTECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Differences in yeast strains lead to differences in strain tolerance to desiccation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] High Density Culture:

[0025] a, preparation of seed liquid: mark the Kluyveromyces marxe bacteria on the YEPD medium plate, place it in a 28°C incubator and cultivate it for 1 day, pick the full strains and mark it on the YEPD medium plate again, Place the culture in the incubator at 28°C for 1 day, then insert the strain into the YEPD liquid medium, culture at 28°C, shake the flask at 160r / min overnight, and obtain the seed liquid;

[0026] b. Preparation of fermentation medium: Weigh 67.37g of sucrose, 29.7g of yeast extract powder, 15.61g of corn steep liquor, 4.13g of potassium dihydrogen phosphate, and 0.3g of magnesium sulfate, dissolve them in 1L of distilled water, mix well, and the temperature is 121°C Sterilize for 20 minutes;

[0027] c. High-density fermentation culture: the seed liquid obtained in step a is inserted into the fermentation medium after step b is sterilized and cooled, and the inoculum size is 3×10 6 cfu / mL, the culture temperature is 28°C...

Embodiment 2

[0033] High Density Culture:

[0034] a, preparation of seed liquid: mark Kluyveromyces marxe on the YEPD medium plate, place it in a 30° C. incubator and cultivate it for 2 days, pick the full bacterial strain and mark it on the YEPD medium plate again, Place the culture in an incubator at a temperature of 30°C for 2 days, then insert the strain into the YEPD liquid medium, and culture in a shaker flask at a temperature of 30°C and 180r / min overnight to obtain a seed liquid;

[0035] b. Preparation of fermentation medium: Weigh 67.37g of sucrose, 29.7g of yeast extract powder, 15.61g of corn steep liquor, 4.13g of potassium dihydrogen phosphate, and 0.3g of magnesium sulfate, dissolve them in 1L of distilled water, mix well, and the temperature is 121°C Sterilize for 20 minutes;

[0036] c. High-density fermentation culture: the seed liquid obtained in step a is inserted into the fermentation medium after step b is sterilized and cooled, and the inoculum size is 3×10 6 cfu / ...

Embodiment 3

[0042] High Density Culture:

[0043] a, preparation of seed liquid: mark Kluyveromyces marxe on the YEPD medium plate, place it in a 29° C. incubator and cultivate it for 2 days, pick the full bacterial classification and mark it on the YEPD medium plate again, Place the culture in an incubator at a temperature of 29°C for 2 days, then insert the strain into the YEPD liquid medium, and culture in a shaker flask at a temperature of 29°C and 170r / min overnight to obtain a seed liquid;

[0044] b. Preparation of fermentation medium: Weigh 67.37g of sucrose, 29.7g of yeast extract powder, 15.61g of corn steep liquor, 4.13g of potassium dihydrogen phosphate, and 0.3g of magnesium sulfate, dissolve them in 1L of distilled water, mix well, and the temperature is 121°C Sterilize for 20 minutes;

[0045] c. High-density fermentation culture: the seed liquid obtained in step a is inserted into the fermentation medium after step b is sterilized and cooled, and the inoculum size is 3×10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing a freeze-dried microbial agent of kluyveromyces marxianus and application of the freeze-dried microbial agent. The freeze-dried microbial agent is prepared by the aid of high-density fermentation culture and freeze-drying technologies. The kluyveromyces marxianus is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms, called as CGMCC for short, on April 27th, 2010, the preservation number of the kluyveromyces marxianus is CGMCC NO.3781, and the strain number is A3. The method and the application have the advantages that the viable cell number of high-density culture solution of the kluyveromyces marxianus can reach 3.2*10<8> cfu / mL at least and is higher than culture results of YEPD (yeast peptone dextrose) culture media by 1.1 order of magnitude at least; zymocyte solution is centrifuged, then bacterial sludge is collected and is added into cryoprotectant solution, the freeze-dried microbial agent can be prepared by the aid of the vacuum freeze-drying technologies, protectants for the freeze-dried microbial agent comprise maltose and skimmed milk powder, and the freeze-dried viable bacterium rate can reach 75% at least; the freeze-dried microbial agent is high in bacterium content, long in storage time, convenient to use and free of pollution, is environmentally friendly and can be used for fermenting milk beer and cheese and making whey wine, fruit vinegar, fruit juice and the like, processes for preparing the freeze-dried microbial agent are simple, and the like.

Description

technical field [0001] The invention relates to a preparation method and application of a freeze-dried bacterial agent of Kluyveromyces marxe, belonging to the field of production of yeast products. Background technique [0002] As a yeast that widely exists in fermented milk, Kluyveromyces marx can not only ferment lactose, but also utilize galactose. During the fermentation process, it can be used alone or together with lactic acid bacteria to ferment milk, giving the product a unique flavor and nutritional properties. At present, the application of Kluyveromyces marxis at home and abroad is mainly focused on the research and development of new dairy products and the preparation of flavor substances such as esters, carboxylic acids, alcohols, and isoamyl acetate. The invention patent applied by Xinjiang Tianrun Biotechnology Co., Ltd. <Preparation of a high-quality yeast and its milk beer beverage> (Patent No. ZL201010256427.X) announced the use of Kluyveromyces mar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/04C12C12/00C12C5/00C12R1/645
CPCC12C5/00C12C12/006C12C2200/01C12N1/04C12N1/16
Inventor 刘朋龙王亮胡曼刘让胡刚尤宏罗晓红詹涛李阳
Owner XINJIANG TIANRUN BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com