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Culture medium for increasing contents of flavonoid compound and polysaccharide of moghania macrophylla tissue culture seedlings

A technology of flavonoids and culture medium, applied in fertilizer mixture, application, production of bulk chemicals, etc., to achieve the effects of reasonable collocation, improved transplanting survival rate, and rapid rhizome growth.

Inactive Publication Date: 2017-08-08
LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a culture medium for increasing flavonoids and polysaccharides in tissue cultured plantlets of Echinacea chinensis to solve the problem of how to increase the content of flavonoids and polysaccharides in the main active ingredients of C. Economic benefits and other issues

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0086] The preparation method of the culture medium for improving flavonoids and polysaccharides in tissue cultured plantlets of E. japonicus comprises the following steps:

[0087] S1: the preparation of ginkgo flavonoids, comprising the following steps:

[0088] S11: crush the dried ginkgo leaves and pass through a 40-80 mesh sieve to obtain ginkgo leaf powder;

[0089] S12: Extract the ginkgo leaf powder prepared in step S11 with water at 62-75°C, the solid-liquid ratio in the extraction is 1:12-16 (W / V), the microwave power is 200-800W, and the extraction time is 15-30min, extract 2-3 times, filter after extraction, and combine the filtrate to obtain a mixed extract;

[0090] S13: Concentrate and dry the mixed extract prepared in step S12 to obtain an extract, which is dissolved in water at 52-55°C with 5.5-6 times the volume of the extract, and extracted with water-saturated n-butanol 2-3 times, continue to carry out multiple crystallization treatments with n-butanol sa...

Embodiment 1

[0096] A culture medium for increasing flavonoids and polysaccharides in tissue-cultured plantlets of E. japonicus, in parts by weight, comprising the following raw materials: 30 parts of sugar, 15 parts of ginkgo flavonoids, 2.5 parts of potassium salt, 2 parts of phosphorus salt, 1.5 parts of nitrogen salt, 0.7 part of calcium salt, 0.7 part of magnesium salt, 03 parts of iodine salt, 0.3 part of boron salt, 0.3 part of molybdenum salt, 0.3 part of zinc salt, 0.3 part of manganese salt, 0.3 part of copper salt, 0.3 part of iron salt, Vitamin 0.18, amino acid 0.18, auxin 0.08, gibberellin 0.08, succinimide 0.06, cytokinin 0.05, kinetin 0.07, conditioner 0.18, dispersant 1.7, synergist 0.04 parts of agent, 1.4 parts of stabilizer, 2 parts of antibacterial agent, 2500 parts of water;

[0097] The sugar is sucrose;

[0098] Described potassium salt is potassium chloride, potassium sulfate, potassium nitrate, potassium carbonate, potassium bicarbonate;

[0099] The phosphorus sal...

Embodiment 2

[0127] A culture medium for improving flavonoids and polysaccharides in tissue-cultured plantlets of E. japonicus, in parts by weight, comprising the following raw materials: 26 parts of sugar, 10 parts of ginkgo flavonoids, 2 parts of potassium salt, 1 part of phosphorus salt, 1 part of nitrogen salt, 0.6 part of calcium salt, 0.6 part of magnesium salt, 0.2 part of iodine salt, 0.2 part of boron salt, 0.2 part of molybdenum salt, 0.2 part of zinc salt, 0.2 part of manganese salt, 0.2 part of copper salt, 0.2 part of iron salt, 0.1 part of vitamin, 0.08 part of amino acid, 0.05 part of auxin, 0.06 part of gibberellin, 0.04 part of succinimide, 0.03 part of cytokinin, 0.04 part of kinetin, 0.12 part of conditioner, 1.2 part of dispersant, synergist 0.02 parts of agent, 0.9 parts of stabilizer, 1.5 parts of antibacterial agent, 2000 parts of water;

[0128] The sugar is sucrose;

[0129] The potassium salt is potassium chloride;

[0130] The phosphorus salt is calcium phospha...

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Abstract

The invention discloses a culture medium for increasing contents of flavonoid compound and polysaccharide of moghania macrophylla tissue culture seedlings. The culture medium is prepared from the following raw materials of sugar, ginkgetin, potassium salt, phosphor salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamins, amino acids, auxin, gibberellin, succinimide, cytokinin, kinetin, a conditioner, a dispersant, a synergist, a stabilizer, an antibacterial agent and water. The culture medium for the moghania macrophylla tissue culture seedlings has the advantages that the compounding is reasonable, the comprehensive nutrients are realized, the root promoting and stem producing can be quickly performed, and the transplanting survival rate is increased; the accumulation of the flavonoid compound and the polysaccharide is promoted, and the economic benefit is increased.

Description

technical field [0001] The invention belongs to the technical field of planting of Echinacea macrophylla, in particular to a culture medium for increasing flavonoids and polysaccharides of tissue cultured plantlets of Echinacea macrophylla. Background technique [0002] Macrophylla (M.ghania macrophylla (Willd.) O. Kuntze) is the dried root of the leguminous plant Macrophylla macrophylla. It is recorded in the appendix of "The Pharmacopoeia of the People's Republic of China" (2010 edition one) as one of the varieties sources of the medicinal material Qianjinba. Qianjinba was first recorded in "Plant Names and Facts", and it is sweet in taste, astringent and flat in nature. Return liver, kidney channel. It has the functions of invigorating the liver and kidney, dispelling rheumatism, and strengthening the waist and knees. For the treatment of rheumatic arthralgia, low back pain, chronic bronchitis, chronic nephritis, infantile malnutrition, spermatorrhea, gynecological dis...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05B7/00C05G3/00C05G3/80C05G5/20C05B9/00C05B17/00C05C1/00C05C3/00C05C5/02C05C11/00C05D1/00C05D1/02C05D3/00C05D5/00C05D9/02C05F11/00C05F11/02C05F11/10Y02P20/54
Inventor 蔡锦源熊建文韦坤华孙松张佳绝谢琳
Owner LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH
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