High-stability single-reagent liquid-state blood ammonia (AMM) detection reagent
A technology with strong stability and detection reagents, which is applied in the field of blood ammonia (AMM) detection, can solve problems such as electrode maintenance and replacement, instability, and increased operating errors, and achieve simplified operation, good protection, and improved accuracy Effect
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Embodiment 1
[0046] A kind of blood ammonia detection reagent of existing three reagents:
[0047] Three reagents:
[0048] Reagent 1a (R1a):
[0049] Tirs buffer PH8.0 50mmol / L
[0050] LDH 2KU / L
[0051] EDTA 5.3mmol / L
[0052] Reagent 1b (R1b):
[0053] α-KG 18.0mmol / L
[0054] NADH0.18mmol / L
[0055] Reagent 2 (R2):
[0056] GLDH 25.0KU / L
Embodiment 2
[0058] The composition of the blood ammonia detection reagent of a single reagent with strong stability is as follows:
[0059] TES (N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid) buffer ····················································· 50mmol / L,
[0060] LDH (lactate dehydrogenase) ·············································································································· 5KU / L
[0061] CDTA ······································································································································ 3g / L
[0062] potassium chloride ····································································································································· 2g / L
[0063] Chloramphenicol ·································································································································· 0.1g / L
[0064] Alpha-ketoglutarate (α-KG) ···················································...
Embodiment 3
[0071] 1) This example describes a single-reagent blood ammonia detection reagent with increased stability of key raw materials. The composition is as follows :
[0072] TES (N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid) buffer ··················································· 50mmol / L,
[0073] LDH (lactate dehydrogenase) ·········································································································· 10KU / L
[0074] CDTA ·································································································································· 10g / L
[0075] potassium chloride ··································································································································· 5g / L
[0076] Chloramphenicol ··································································································································· 1g / L
[0077] Alpha-ketoglutarate (α-KG) ··············...
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