Injectable temperature-sensitive magnetic nano emulsive gel and preparation method and application thereof

Abstract

The invention discloses a preparation method of an injectable temperature-sensitive magnetic nano emulsive gel. The preparation method comprises the following steps: by taking a mixture of silicone oil and ZnFe2O4 nanoparticles as an oil phase and an aqueous solution where lauryl sodium sulfate is dissolved as an aqueous phase, dropwise adding the oil phase into the boiling aqueous phase while stirring to form an initial emulsion; performing uniform nanocrystallization on the initial emulsion by using an ultrasonic cell crusher to obtain a magnetic nanoemulsion; dropwise adding polyethylene glycol diacrylate (PEG-DA) into the magnetic nanoemulsion under a low temperature condition, performing uniform ultrasonic dispersion, and fully cooling the mixture to obtain the magnetic nano emulsive gel which is good in flowability at normal temperature (less than 25 DEG C) and is gelatinized at body temperature (37 DEG C). The magnetic nano emulsive gel prepared by the invention can be injected in situ in a subcutaneous tumor of a mouse under guidance of an image and is gelatinized and fixed in the part of tumor, without leaking from an injection hole or diffusing to peripheral normal tissues and blood vessels; by applying an alternating magnetic field, the ZnFe2O4 nanoparticles generate heat to cause thermal ablation of tumor tissues for necrosis and exfoliation. The method has a wide application prospect in solid tumor interventional thermotherapy.

Description

Technical field [0001] The invention belongs to the biological and medical nanomaterials and technical fields, and specifically relates to a preparation method and application of an injectable temperature-sensitive magnetic nanoemulsion gel. Background technique [0002] Nanoemulsion is a thermodynamically stable system with a particle size of 10-100nm formed by water, oil, surfactant and co-surfactant in an appropriate proportion. The main types are water-in-oil, oil-in-water and bicontinuous . The emulsion droplets are mostly spherical, uniform in size, and transparent or slightly opalescent liquid in appearance. The main preparation methods of nanoemulsion include shear stirring method, high-pressure homogenizer homogenization method, ultrasonic method and phase change method, etc., which are easy to prepare, simple to operate, long-term storage without layering and demulsification, and can be filtered and sterilized. As a new type of drug carrier, nanoemulsion is small in s...

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Problems solved by technology

Intravenous injection is through the long circulation in the body, using the tumor-enhanced osmotic retention effect (EPR effect) to enrich in the tumor area, but the dose of nanoparticles reaching the tumor is very small, most of which are trapped by the liver and spleen, and some magnetic nanomaterials heat up Due to the lack of performance, it is usually necessary to apply a high-frequency magnetic field and multiple times of magnetic hyperthermia to obtain a certain effect, which reduces the effect of tumor hyperthermia and also damages normal tissues; intratumoral injection increases the content of nanoparticles in tumor tissues , the treatment effect can be improved, but the problem it brings is that the intratumoral pressure of the tumor tissue makes it easy for the injected magnetic nanoparticles to leak through the needle hole of the syringe, and it is easy to diffuse from the tumor tissue to the surrounding normal tissue, making the normal tissue in the treatment process The lack of image guidance during the operation also makes the injection position prone to deviation and affects the therapeutic effect

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