Method for improving lignocellulose enzymolysis efficiency by lignocellulose pretreatment through ultrasonic-microwave synchronously-assisted ionic liquid system

A lignocellulose and lignocellulose technology is applied in the field of resource utilization of lignocellulose biomass, which can solve the problems of high cost and high energy consumption, and achieve the effects of reducing dosage, saving resources and reducing costs.

Inactive Publication Date: 2017-04-26
HUAIYIN TEACHERS COLLEGE +1
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: in the pretreatment process of lignocellulose by using ionic liquid, it is necessary to control the water content in the ionic liquid in a very small range, so as to promote the dissolution of cellulose in the ionic liquid. Long-term high-temperature dehydration treatment of raw materials and ionic liquids, high energy consumption, high cost and other issues

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  • Method for improving lignocellulose enzymolysis efficiency by lignocellulose pretreatment through ultrasonic-microwave synchronously-assisted ionic liquid system

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Using [Cho][Orn] to pretreat rice straw to improve its enzymatic hydrolysis efficiency.

[0028] a) Pretreatment: Accurately weigh 0.25 g rice straw powder (150-350 μm) and 4 g [Cho][Orn], put them together in a 20 mL Erlenmeyer flask, stir at 95°C for 12 hours; then cool to room temperature , take 0.5 mL supernatant, and use UV spectrophotometry to measure lignin extraction rate.

[0029] b) Subsequent sample preparation: Slowly add NaOH solution (0.1mol / L) 1 times the volume of the ionic liquid to the pretreatment system to dilute, filter, and then wash the filter residue 4 times with 2 times the volume of deionized water until the supernatant is colorless , and the filter residue was dried at 60°C to obtain the pretreated rice straw sample.

[0030] c) Enzyme hydrolysis: Accurately weigh 0.05 g of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 20 mL citrate buffer (50 mmol / L, pH 4.8) and 45 U commercial Cellulase was...

Embodiment 2

[0031] Example 2: Using [Cho][Orn]-water mixed system to pretreat rice straw to improve its enzymatic hydrolysis efficiency.

[0032]a) Pretreatment: Accurately weigh 0.25g of rice straw powder (150-350μm), 2g of [Cho][Orn] ionic liquid and 2g of deionized water, put them together in a 20mL Erlenmeyer flask with stopper, at 95℃ The mixture was stirred for 12 hours, and then cooled to room temperature; 0.5 ml of the supernatant was taken to determine the lignin extraction rate by ultraviolet spectrophotometry.

[0033] b) Subsequent sample preparation: Slowly add 1 times the volume of ionic liquid NaOH solution (0.1mol / L) to the pretreatment system to dilute, filter, and then wash the filter residue 4 times with 2 times the volume of deionized water until the supernatant is colorless , and the filter residue was dried at 60°C to obtain the pretreated rice straw sample.

[0034] c) Enzymatic hydrolysis: Accurately weigh 0.05 g of the above-mentioned pretreated rice straw, place...

Embodiment 3

[0035] Example 3: Ultrasonic-microwave-assisted [Cho]Orn-water mixed system (50%:50%) pretreats rice straw to improve its enzymatic hydrolysis efficiency.

[0036] a) Pretreatment: Accurately weigh 0.25 g of rice straw powder (150–350 μm), 2 g of [Cho][Orn] ionic liquid and 2 g of deionized water, put them together in a 20 mL Erlenmeyer flask, and place them in an ultrasonic-microwave In a synergistic reactor (Nanjing Xianou Instrument Manufacturing Co., Ltd.); the setting conditions are: ultrasonic frequency 25KHZ, power 800W; microwave frequency 2450 MHZ, power 1000W, and keep at 95°C for 6 hours. After cooling to room temperature, 0.5 ml of the supernatant was taken to determine the lignin extraction rate by ultraviolet spectrophotometry.

[0037] b) Subsequent sample preparation: Slowly add NaOH solution (0.1 mol / L) 1 times the volume of the ionic liquid to the pretreatment system to dilute, filter, and then wash the filter residue 4 times with 2 times the volume of deioni...

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Abstract

The invention belongs to the technical field of resourceful utilization of lignocellulose biomass, aims to provide an environment-friendly efficient lignocellulose pretreatment method and relates to a novel method for improving lignocellulose enzymolysis efficiency by extracting lignin components in lignocelluloses through an ionic liquid-water mixed system assisted by synergistic effect of ultrasonic-microwave energy. Compared with a traditional ionic liquid pretreatment method, the method has the advantages that synergistic ultrasonic-microwave energy is initiatively used for increasing the ratio that the ionic liquid-water mixed system extracts the lignin, enhancing accessibility of cellulase and the cellulose components in the lignocelluloses and improving the lignocellulose enzymolysis efficiency; ionic liquid consumption during pretreatment is reduced substantially through mixing of water, so that technological costs are saved; the method is short in treatment time, mild and green in conditions and simple in technology.

Description

technical field [0001] The invention relates to a method for synchronously assisting an ionic liquid-water mixed system to pretreat lignocellulose to improve its enzymatic hydrolysis efficiency, belonging to the technical field of resource utilization of lignocellulosic biomass. Background technique [0002] Lignocellulose is a huge biomass resource on the earth, and its main sources include crop straw, trees, energy plants and weeds. With the emergence of a series of environmental and energy problems, renewable lignocellulose has become a key energy resource for human development and utilization. Due to its low energy utilization rate and serious environmental pollution, the traditional utilization form of directly burning lignocellulose has been gradually replaced by other methods; Fermentation to obtain fuels and other chemicals has become a new research hotspot. [0003] Enzymatic hydrolysis of lignocellulose to prepare reducing sugars is favored due to its environme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/14C12P19/02C12P2201/00C12P2203/00
Inventor 许家兴高文华徐继明周守勇赵宜江夏军刘晓燕
Owner HUAIYIN TEACHERS COLLEGE
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