Three-dimensional prevention and control method for desertification using biological soil crust-plant system

A biological soil and crust technology, applied in the construction of artificial biological soil crusts, can solve problems such as water shortage, reduce wind and water erosion, promote the restoration of ecological environment, and reduce the effects of wind and water erosion.

Inactive Publication Date: 2017-03-29
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for three-dimensionally preventing and controlling desertification by using the biological soil crust-plant system, which effectively solves the problem of water shortage in the construction of the biological soil crust-plant system in the desert, and prevents wind erosion and water erosion It promotes the formation and development of desert vegetation, and accelerates the restoration of the ecological environment in desert areas.

Method used

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  • Three-dimensional prevention and control method for desertification using biological soil crust-plant system

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1: Isolation and identification of microorganisms

[0033] Weigh 5g of biological soil crusts collected from Inner Mongolia into a 100mL conical flask, add 45mL of sterile water, the dilution concentration at this time is 10-1, weigh 0.5mL of soil suspension with a concentration of 10-1 in 10mL of soil suspension. In a sterile centrifuge tube, mix well, at this time the concentration is 10-2, take samples in turn, and dilute to a concentration of 10 -5 , take 100 μL of the diluted soil suspension and place it in solid medium such as 1 / 2R2A, Ashby, and dephosphorization, spread it, and place it in a light incubator for cultivation. After obtaining the pure cultures of photosynthetic bacteria, nitrogen-fixing bacteria, phosphate-solubilizing bacteria and cyanobacteria, extract their DNA, and select primers

[0034] 27F: 5'-AGAGTTTGATCMTGGCTCAG-3', see SEQ ID NO: 1,

[0035] 1492R: 5'-GGTTACCTTGTTACGACTT-3', see SEQ ID NO: 2, its 16S rDNA was amplified by PCR, th...

Embodiment 2

[0036] Example 2: Preliminary analysis of microbial functional genes

[0037] Use pufM and nifH to verify whether it has the functions of utilizing light energy and nitrogen fixation, respectively. using primers

[0038] puf M_uni F: 5'-GGNAAYYTNTWYTAYAAYCCNTTYCA-3', see SEQ ID NO: 3,

[0039] pufM_uni R: 5′-YCCATNGTCCANCKCCARAA-3′, see SEQ ID NO: 4, amplify the pufM functional gene of the isolated microorganism; use primers

[0040] FGPH273: 5′-CTC CGG GCC RCC NGA YTC-3′

[0041] , see SEQ ID NO: 5,

[0042] pol R 5′-ATS GCC ATC ATY TCR CCG GA-3′

[0043] , see SEQ ID NO: 6,

[0044] pol F: 5'-TGC GAY CCS AAR GCB GAC TC-3', see SEQ ID NO: 7,

[0045] AQER: 5'-GAC GAT GTA GAT YTC CTG-3', see SEQ ID NO: 8, the isolated microorganism was subjected to amplification of the nifH functional gene.

Embodiment 3

[0046] Example 3: Screening of siderophore strains

[0047] CAS plate formula: CAS blue staining solution contains 1mmol / L CAS (chrome azure), 0.1mmol / L FeCl 3 , 4mmol / L cetyltrimethylamine bromide, 0.1% phosphate buffer at pH 6.8, 3g KH per 100mL MM9 salt solution 2 PO 4 , 5g NaCl, 10g NH 4 Cl.

[0048] CAS solid medium: Take 100mL MM9 salt solution in 750mL deionized water, dissolve 32.24g 2-2 sulfonic acid, pH is 6.8, add 15g agar, sterilize at high temperature and autoclave, cool to 50℃, add 30mL sterilized casein The hydrolyzate and 10 mL of 20% glucose were added to the MM9 / 2-2 sulfonic acid mixture, and 10 mL of CAS blue dye solution was slowly added until the substrate was mixed, and finally a solid plate was made. The activated strains were inoculated into the CAS solid medium by streaking, and the growth of the strains was observed during the period. The strains with orange halo were siderophore-producing strains. The experimental results showed that among the 5...

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Abstract

A three-dimensional prevention and control method for desertification using a biological soil crust-plant system. The method comprises following steps: (1) water collecting pipe preparation; (2) supporting layer preparation; (3) functional bacteria composite system suspension preparation; (4) protection layer preparation. The three-dimensional prevention and control method for desertification using a biological soil crust-plant system has the water collecting and water conservation effect, which can reduce the influences of wind erosion and water erosion; the method realizes fast generation and well development of biological soil crust, realizes germination and growth of plants, increases building rate and coverage of vegetation in desert, effectively prevents and controls desertification and promotes ecological environment recovery in desert regions.

Description

technical field [0001] The invention relates to a method for constructing artificial biological soil crusts, in particular to a method for three-dimensional control of desertification by utilizing a biological soil crust-plant system. Background technique [0002] Desertification refers to the long-term unreasonable human activities interacting with the fragile ecological environment, resulting in the decline of land productivity, soil erosion, and the phenomenon of desert-like landscapes on the surface. Desertification is an important environmental problem faced by human beings in today's society, and the formation and development of biological soil crusts (biological soil crusts, also known as microbial crusts and biological crusts) provide a good foundation for the restoration of the ecological environment in desert areas. The key initial process of ecological environment restoration in desert areas is the primary symbol of the stability of mobile sand dunes, and is an im...

Claims

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Application Information

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IPC IPC(8): A01B79/02
CPCA01B79/02Y02A40/22
Inventor 冯福应贾丽娟唐凯杨杉杉张胜男李蘅孟建宇
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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