Culture method of ganoderma leucocontextum liquid strain

Abstract

The invention discloses a large-scale production method of a ganoderma leucocontextum liquid strain. The method comprises the following steps: finely selecting a naturally growing ganoderma leucocontextum fruiting body, and conducting tissue isolation so as to obtain the pure strain. Based upon an artificial cultivation test, preferably, the artificially cultivated ganoderma leucocontextum fruiting body is bred, and through 5-10 generations of systematic breeding, an original ganoderma leucocontextum mother strain, which is good in stability, strong in metabolic capability and high in production efficiency, is obtained. The mother strain is prepared with the adoption of a solidified medium and a liquid medium, and the fermentation culture of ganoderma leucocontextum mycelia is conducted with the application of a liquid medium in accordance with a liquid strain culture method, so that the large-scale production of ganoderma leucocontextum stock and cultivated strains is achieved. The method is low in cost and easy to implement, and the method can be widely applied to the large-scale artificial production of the ganoderma leucocontextum. The culture medium and the liquid strain production method provided by the invention are especially suitable for the strain preparation of the ganoderma leucocontextum and the large-scale liquid fermentation culture of the mycelia; and with the application of the culture medium and the production method, the fermentation culture of the ganoderma leucocontextum mycelia can be conducted rapidly, so that the sustainable utilization of a ganoderma leucocontextum natural resource is achieved.

Description

technical field [0001] The invention belongs to the technical field of domestication and cultivation of wild edible fungi, in particular to a Yunnan white ganoderma (Ganoderma leucocontextum) A method for cultivating liquid strains. Background technique [0002] Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine in the motherland, and is known as the "immortal grass". The "Shen Nong's Materia Medica" in the Eastern Han Dynasty and the "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty all have detailed and very positive records of the efficacy of Ganoderma lucidum, which is also confirmed in traditional Chinese medicine. Ganoderma lucidum contains a variety of active ingredients such as polysaccharides, steroids, triterpenoids, bases, nucleosides, shellac acid, organic germanium, and various alkaloids, which have the effects of regulating immunity, anti-tumor, and anti-cancer. Ganoderma lucidum is a traditional and preci...

AI Technical Summary

Problems solved by technology

[0004] In order to develop rare Yunnan white ganoderma resources and utilize the favorable conditions in this area to develop the production of edible and medicinal fungi, the purpose of this invention is to cultivate the original female species of Yunnan ganoderma lucidum with good stability, strong metabolic capacity and high production efficiency and provide a new The large-scale cultivation method of liquid strains for the cultivation of Yunnan white ganoderma lucidum, which can quickly carry out liquid fermentation culture of Yunnan white ganoderma filaments, and solves the problem of strain supply for the artificial cultivation and industrialization development of Yunnan ganoderma lucidum