Galactosyl grafted-modified alginate microspheres and applications thereof

A technology of galactose and alginate, which is applied in the direction of fixing on/in the organic carrier, can solve problems such as the inability to introduce galactose groups, and achieve the maintenance of immune isolation performance, excellent biocompatibility, and conditions mild effect

Inactive Publication Date: 2017-02-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitation of the stability of the microcapsules and the degree of substitution of the galactosyl group, no matter the method of blending or covalently modifying alginic acid, it is impossible to introduce a higher concentration of galactose groups inside the microcapsules.

Method used

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  • Galactosyl grafted-modified alginate microspheres and applications thereof
  • Galactosyl grafted-modified alginate microspheres and applications thereof
  • Galactosyl grafted-modified alginate microspheres and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1) Preparation of alginic acid solution: Alginic acid was dissolved in water to prepare a 1 g / L alginic acid solution with a volume of 500 mL. Among them, the molecular weight of alginic acid is 350kDa.

[0045] 2) Filter the solution of 1) to remove impurities, and adjust the pH of the alginic acid solution to 10 with a mass concentration of 240g / L NaOH solution.

[0046] 3) Dissolve 0.06g of CNBr solid with 0.5mL of acetonitrile and add it dropwise into the alginic acid solution in step 2), and keep the pH value constant.

[0047] 4) Cleaning the activated solution prepared in step 3) until the unreacted CNBr small molecule is removed.

[0048] 5) Add 0.4 g of lactosamine solid to the washed solution, and stir at room temperature for 2 days.

[0049] 6) Washing the solution after the reaction in step 5) until unreacted lactosamine molecules are removed.

[0050] 7) Freeze-drying the washed solution in step 6) to obtain galactosyl alginic acid with a grafting degree...

Embodiment 2

[0079] 1) Preparation of sodium galactosyl alginate solution: galactosyl alginic acid was dissolved in physiological saline to prepare a 15 g / L sodium galactosyl alginate solution, wherein the molecular weight of galactosyl alginate was 350 kDa.

[0080] 2) Preparation of chitosan solution: chitosan was dissolved in acetic acid-sodium acetate buffer solution with a pH of 6.0, and the concentration of chitosan was 5 g / L. Among them, the molecular weight of chitosan is 30kDa, and the degree of deacetylation is 90%.

[0081] 3) Preparation of gel bath solution: Anhydrous calcium chloride was dissolved in deionized water to prepare a 11 g / L calcium chloride solution.

[0082]4) Preparation of galactosyl calcium alginate / chitosan microcapsules: prepare galactosyl calcium alginate embedded hydrogel microspheres by high-voltage electrostatic method, and immerse the embedded hydrogel microspheres in chitosan solution In this method, the volume ratio of embedded hydrogel microspheres ...

Embodiment 3

[0085] 1) Preparation of galactosyl sodium alginate solution: galactosyl alginic acid was dissolved in physiological saline to prepare a 15 g / L galactosyl sodium alginate solution, which was sterile filtered. Among them, the molecular weight of galactosyl alginic acid is 350kDa.

[0086] 2) Preparation of α-polylysine solution: α-polylysine was dissolved in physiological saline to prepare a 5 g / L α-polylysine solution, which was sterile filtered. Among them, the molecular weight of α-polylysine is 30kDa.

[0087] 3) Preparation of gel bath solution: Anhydrous calcium chloride was dissolved in deionized water to prepare a 11 g / L calcium chloride solution, which was sterilized by filtration.

[0088] 4) Prepare galactosyl calcium alginate / α-polylysine-embedded hydrogel microsphere carrier that wraps liver cells: take 3mL galactosyl sodium alginate solution, add 6*10 ^6 Rat primary hepatocytes were mixed evenly, and galactosyl calcium alginate-embedded hydrogel microspheres emb...

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Abstract

The present invention relates to a galactosyl grafted-modified alginate hydrogel microsphere carrier preparation method. According to the present invention, the occupation on the hydroxyl site of alginic acid does not affect the gelation performance of the alginic acid, such that the embedding type hydrogel microsphere carrier with characteristics of good sphericity and good stability can be prepared from the galactosyl alginic acid being subjected to the grafting reaction, and can further be subjected to the film forming reaction with polycations to form the galactosyl alginate/polycation microcapsule.

Description

technical field [0001] The invention relates to an alginate microsphere carrier product, in particular to a galactose group graft-modified alginate microsphere carrier. Background technique [0002] Encapsulate biologically active substances in selective permeable membranes to form spherical microcapsules, which are called "biological microcapsules" [Chang TMS.Hemoglobin corpuscles.Research report honors physiology.Medical Library,McGill University,1957] . Using biological microcapsules as the immune isolation and delivery tool of cells, using genetically recombined cells or metabolites of primary cells to regulate the body's physiological functions and treat related diseases is a research hotspot for biomedical workers [Basic D, Vacek I, Sun A M. Microencapsulation and transplantation of engineered cells: a new approach to somatic gene therapy. Art Cells Blood Subs Immob Biotechnol, 1996, 24(3): 219-255]. Alginate has become the main material of microencapsulation technol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08C12N11/04
Inventor 于炜婷娄茹云刘晓岑马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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