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Oryza sativa MIT1 gene as well as encoded protein and application thereof

A rice and protein technology, applied in the field of genetic engineering, can solve the problems of plant tillering or branch number increase, plant height reduction, etc., and achieve the effects of inhibiting tillering, increasing productivity, and huge economic value

Active Publication Date: 2017-02-15
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mutations in strigolactone pathway genes often result in an increase in the number of tillers or branches in plants, accompanied by a decrease in plant height

Method used

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  • Oryza sativa MIT1 gene as well as encoded protein and application thereof
  • Oryza sativa MIT1 gene as well as encoded protein and application thereof
  • Oryza sativa MIT1 gene as well as encoded protein and application thereof

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Experimental program
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Effect test

Embodiment 1

[0037] The acquisition and phenotype analysis of embodiment 1 mutant

[0038] mutant mit1 is 60 Co-γ radiation mutagenesis japonica rice variety Chunjiang 06 (irradiation dose 200Gy, dose rate 10Gy·h -1 ), phenotypically it has many changes compared with the wild type. The mutant mit1 exhibited a phenotype of moderately increased tillers and semi-dwarf plant height. The plant height at maturity was slightly shorter, about 80% of the wild type, and the internodes were shortened to a certain extent; the number of tillers increased, about 80% of the wild type. type 2 times (see figure 1 ); the plant type is compact, and the tiller angle and leaf angle are small; at different development stages after transplanting, the plant height of mit1 is lower than that of wild type, and the tiller is more than that of wild type (see figure 2 ). The fertility of the mutant mit1 is normal, and the number of grains per panicle is about 70% of the wild type, but the total number of grains p...

Embodiment 2

[0039] Example 2 Acquisition of Rice MIT1 Gene and Verification of Functional Complementation

[0040] 1. Acquisition of rice MIT1 gene

[0041] The MIT1 gene of the present invention is obtained by cloning the mutant mit1 by adopting a map position cloning method.

[0042] through 60 Mutant mit1 induced by Co-γ radiation has been confirmed to have stable inheritance characteristics after multiple generations of self-planting. Homozygous mutant mit1 was crossed with normal phenotype indica Dular, all crosses F 1 Medium individuals were dwarf and moderately increased tiller phenotype. in F 2 There was obvious segregation in the planting population. Through investigation and analysis, it was found that the segregation ratio of the wild type and the mutant was in line with the 3:1 genetic segregation ratio. Therefore, it can be inferred that the mutant traits of the rice mutant with moderately increased dwarf tillering are controlled by a pair of recessive genes.

[0043] I...

Embodiment 3

[0051] Example 3 Homologous protein sequence alignment and evolution analysis of MIT1

[0052] Sequence alignment of rice Z-ISO (MIT1), maize Z-ISO (ZmZ-ISO) and Arabidopsis Z-ISO (AtZ-ISO) showed relatively high homology. According to the prediction of the rice genome annotation website (http: / / rice.plantbiology.msu.edu / ), MTI1 contains 368 amino acids, ChloroP 1.1Prediction Server predicts that the protein has a chloroplast transit peptide (box part) at the N-terminus, and the whole protein Contains 5 transmembrane regions (underlined), and an NnrU domain (see Figure 4 Figure A).

[0053] Z-ISO is 15-cis-ζ-carotene isomerase, which is one of the key enzymes involved in the biosynthetic pathway of carotenoids. Phylogenetic tree analysis shows that MTI1 has homologous genes in higher plants and diatoms, and they all have a common origin—the NnrU gene, which is found in denitrifying bacteria, such as Agrobacterium fabrumstr.C58 and Sinorhizobium meliloti 1021 (see Figure ...

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Abstract

The invention provides an oryza sativa MIT1 gene as well as encoded protein and an application thereof. A nucleotide sequence of the gene is shown in SEQ ID No.2, and the gene encoded protein sequence is shown in SEQ ID No.1. The gene is expressed in multiple tissue, and MIT1 protein is located in chloroplast. Experiments prove that MIT1 has a function of inhibiting branching of oryza sativa. The invention further provides a mutant gene of the MIT1 gene. According to the mutant gene, 10bp deletion occurs at the junction of the second intron and the third exon of the MIT1 gene. The mutant gene comprises two bases ag at the 3' end of the second intron and eight bases GTCTACTT at the 5' end of the third exon. By means of the mutant gene, the oryza sativa shows to have lower plant height and moderately increased tillering, the yield of the mutant is increased compared with that of the wild oryza sativa, and the mutant oryza sativa is expected to be used as a plant type breeding material. The oryza sativa MIT1 gene as well as the mutant of the MIT1 gene has great significance in genetic improvement breeding of oryza sativa germplasm resources.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a rice MIT1 gene, its encoded protein and its application in inhibiting rice branching. Background technique [0002] Rice (Oryza sativa) is a monocotyledonous plant of the Poaceae family and one of the most important food crops in the world. At present, my country's rice planting area is about 450 million mu, ranking second in the world, with a total output of 204 million tons, ranking first in the world, with a per unit yield of 420 kg, which is 1.6 times the world average. However, as the population continues to grow and the area of ​​arable land continues to decrease, it poses greater challenges to food production. Therefore, tapping the yield potential of crops through genetic improvement of varieties is an effective way to solve the problem of food security, and it is also the fundamental way to ensure the steady growth of world food production. [0003] Til...

Claims

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Application Information

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IPC IPC(8): C12N9/90C12N15/61C12N15/82A01H5/00
Inventor 李学勇刘丽华彭鹏赵金凤房静静
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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