A method for analyzing great bustard food source plants by fecal dna
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A technology of bustards and plants, which is applied in the field of analysis of bustard food source plants through feces DNA, and analysis of bustard food, can solve problems such as lack of scientific guidance, unclear food types and nutritional requirements of bustards, and unsuccessful artificial breeding
Active Publication Date: 2019-11-19
RES INST OF FORESTRY NEW TECH CHINESE ACAD OF FORESTRY
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However, the food types and nutritional requirements of the bustard in each life history period are still unclear, resulting in the lack of scientific guidance for the conservation department to protect wild bustard populations and foraging grounds, which is also the bottleneck of the unsuccessful artificial breeding of the bustard for many years
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Embodiment 1
[0115] Example 1. Analysis of bustard food source plants based on chloroplast DNA trnL barcodes
[0116] (1) Establish a complete sequence database of native plant chloroplast DNA trnL
[0117] 1. Collection of potential food source plants
[0118] In places where the bustard is active or where there are traces of bustard activity (feces, feathers, scratches, etc.), collect plant leaves, put fresh plant leaves in paper bags, add silica gel, and store them dry. Record the family, genus and species information of the collected plants.
[0119] 2. Plant DNA extraction
[0120] Take the leaves and put them in a mortar, grind them into powder with liquid nitrogen, and use a broad-spectrum plant genomic DNA rapid extraction kit (Item No.: DL116-01, Beijing Biomed Gene Technology Co., Ltd.) to extract plant genomic DNA.
[0121] Perform agarose electrophoresis on the extracted DNA, and use a UV spectrophotometer to determine the concentration and purity. For DNA with an OD260 / OD28...
Embodiment 2
[0174] Embodiment 2, the method verification based on fecal DNA analysis bustard eating habits
[0175] (1) Collect known plants to feed the great bustard in cages and collect feces
[0176]In places with great bustard activity or traces of bustard activity (feces, feathers, scratches, etc.) in the wild, 10 plants were collected from 8 species of plants. (Anemarrhenaasphodeloides), Bupleurum chinense, Leymus chinensis, Chloris virgata, Rhaponticum uniflorum, Scabiosa comosa, Potentilla discolor. In addition, it was fed with 2 plant staples, mung bean (Vigna radiata) and maize (Zea mays).
[0177] Before feeding the caged great bustards, old feces, leftover food and weeds on the ground were removed in advance to avoid interfering with subsequent analysis. Six adult great bustards with good daily feeding conditions were selected as experimental subjects, and they were given enough and clean water during the feeding period. The bustard was not fed on the first day, only water ...
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Abstract
The invention discloses a method for analyzing an otis tarda food source plant through feces DNA (Deoxyribonucleic Acid). The method comprises the following steps of (1) using trnL gene amplification primer pairs with different base tags for respectively amplifying multiple trnL gene segments in otis tarda feces to be measured, so as to obtain the multiple trnL gene segments in the otis tarda feces to be measured added with different base tag sequences; (2) mixing the multiple trnL gene segments in the otis tarda feces to be measured added with the different base tag sequences, so as to obtain mixed segments, high-throughput sequencing the mixed segments, so as to obtain multiple sequencing sequences of the trnL gene segments in the otis tarda feces to be measured; (3) comparing the multiple sequencing sequences of the trnL gene segments in the otistarda feces to be measured with an eciophyte chloroplast DNA trnL complete sequence database and a common trnL bar code database, judging the otis tarda food source plant to be measured according to the homology of sequences in the sequencing sequences and the two databases, and calculating the percentage of a plant ingested by an otis tarda according to a sequence bar number. The method for analyzing the otis tarda food source plant through feces DNA provided by the invention provides a technical support for researching feeding habit preference of animals and recipes.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a method for analyzing the food habits of the great bustard, and in particular to a method for analyzing the food source plants of the great bustard by fecal DNA. Background technique [0002] Diet analysis is one of the important topics in wildlife conservation biology research, and it is also the basis for understanding the relationship between animals and habitats, and can provide scientific support for the effective protection and management of wildlife. Traditional methods for analyzing animal feeding habits include field observation of foraging behavior, analysis of gastric contents, and microscopic identification of fecal composition, however, these methods suffer from invasiveness, poor operability, and low accuracy. For example, field observation has poor operability and extensive results, while gastric content analysis can cause damage or even death to animals, which has be...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895
Inventor 刘刚龚明昊崔丽娟李林海宁宇韩·莫日根周景英于长江李惠鑫夏晓飞
Owner RES INST OF FORESTRY NEW TECH CHINESE ACAD OF FORESTRY
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