Method for detecting carotenoid content of tea leaves by confocal micro-Raman

A confocal microscopy and carotene technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of unstable carotenoid properties, destructive properties, cumbersome detection steps, and inability to achieve online detection, etc. Economic benefits, shortened detection time, and simple structure

Active Publication Date: 2017-01-04
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

Traditional detection methods are destructive, the detection steps are cumbersome, and online detection cannot be realized
At the same time, the properties of carotenoids in tea are not very stable, and are easily affected by light, oxygen, and heat during the experiment. Therefore, there is a need for a rapid and non-destructive method for the detection of carotenoids in tea

Method used

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  • Method for detecting carotenoid content of tea leaves by confocal micro-Raman
  • Method for detecting carotenoid content of tea leaves by confocal micro-Raman
  • Method for detecting carotenoid content of tea leaves by confocal micro-Raman

Examples

Experimental program
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Effect test

Embodiment 1

[0063] The sample stage structure provided by the present invention is as Figure 4 As shown, it includes: slide glass 2, cover glass 1 and stage 3. Both the slide glass 2 and the cover glass 1 are made of quartz.

[0064] The surface of slide glass 2 is used to place tea sample, and slide glass 2 is rectangular, and two long sides of slide glass 2 are respectively fixed with a slide block 7, and spring pin is fixed on each slide block 7, and two slide blocks 7 corresponding to the location of the spring pin on the A silicon chip 5 is embedded on the slide glass 2 .

[0065] The cover glass 1 is rectangular, and the long side of the cover glass 1 is hinged with one of the sliders 7, and the cover glass 1 is overturned to compress the tea sample. The cover glass 1 is used to keep the surface of the tea leaves flat, to minimize the variation of the object distance when detecting different points on the tea samples, and to ensure that the Raman detection lens can be accurately...

Embodiment 2

[0068] Take 315 pieces of Longjing 43 leaves. For each Longjing 43 leaf, randomly cut the leaves on both sides of the veins to obtain two tea samples. The mass of each tea sample is about 0.1g. After cutting, the actual measurement and record Ww. One of them is directly placed in the bag and labeled as Raman spectroscopic material A, the other is cut into pieces and placed in a centrifuge tube and labeled, added with 10mL of alcohol solution with a mass fraction of 95%, and stored in a dark room for about 24 hours , as UV spectrophotometric material B. For the same tea leaves, Raman spectroscopic material A and UV spectrophotometric material B have the same label.

[0069] The Raman spectroscopic material A is fixed on the Raman stage described in Example 1, using a Renishaw confocal laser microscope Raman instrument (Renishaw in Via-Reflex 532 / XYZ), the excitation wavelength is 532nm; the laser intensity 50mW; integration time 1s; Raman spectrum detection wavelength range i...

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Abstract

The invention discloses a method for detecting the carotenoid content of tea leaves by a confocal micro-Raman. The method includes the steps: 1) placing a tea leaf sample on a sample table, acquiring Raman spectral information of the tea leaf sample by a Raman spectrometer and performing full-wave band integral compensation; 2) building a linear model by the Raman spectral intensity of 17 characteristic wave numbers; 3) acquiring the corrected spectral intensity of the tea leaf sample to be detected at the 17 characteristic wave numbers, predicting the carotenoid content of the tea leaves by the linear model, and realizing nondestructive and rapid detection of the carotenoid content of the tea leaves. The carotenoid content of the tea leaves can be rapidly and effectively detected in a nondestructive, low-cost and environment-friendly manner without complicated pretreatment and chemical analysis for the tea leaf sample, operation steps are greatly simplified, detection time is shortened, information of carotenoid components in the tea leaves is rapidly acquired, and detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of carotenoid content detection, in particular to a method for detecting carotenoid content in tea by confocal micro-Raman. Background technique [0002] Carotenoids are yellow-red pigments that widely exist in microorganisms, plants, animals and humans, and are essential components of living organisms. Carotenoids have two main functions in the photosynthesis of plants, one is to protect the photosynthetic system under the condition of excessive light irradiation, and the other is to absorb light energy as an antenna dye. [0003] Vegetation pigment content has a good correlation with its photosynthetic ability, developmental stage and nutritional status, and is usually an indicator of vegetation environmental stress, photosynthetic ability and vegetation developmental stage. Carotenoids not only endow various organisms with brilliant colors, but also serve as auxiliary pigments for photosynthesis, which n...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 李晓丽许凯雯何勇周瑞清张裕莹魏玉震
Owner ZHEJIANG UNIV
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