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Three-dimensional porous composite scaffold and preparation method thereof

A composite scaffold, three-dimensional porous technology, applied in medical science, prosthesis and other directions, can solve the problems of unfavorable cell adhesion and proliferation, affecting the porosity and pore size of the scaffold, and affecting the application performance of the scaffold, etc. Value-added, accelerated wound healing, low-cost effects

Active Publication Date: 2016-11-30
BEOGENE BIOTECH GUANGZHOU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improper mixing ratio or improper mixing conditions will affect the porosity and pore size of the scaffold, which is not conducive to the adhesion and proliferation of cells, which will also affect the application performance of the scaffold.

Method used

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  • Three-dimensional porous composite scaffold and preparation method thereof
  • Three-dimensional porous composite scaffold and preparation method thereof
  • Three-dimensional porous composite scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0035] Example 1 Three-dimensional porous composite scaffold and preparation method thereof

[0036] The preparation method of the three-dimensional porous composite scaffold of this embodiment comprises the following steps:

[0037] (1) Take 8g silkworm cocoons and place them in a large beaker, add 400ml deionized water, and add 2g anhydrous sodium carbonate at the same time, boil for half an hour, take out, wash with deionized water several times until neutral, then repeat the above steps, place drying in an oven to obtain silk fibroin for use;

[0038] (2) Add 2.5g of silk fibroin to 25ml of 9.8mol / L lithium bromide solution, heat it in a water bath at 60°C for 5 hours, then dialyze with a dialysis bag (the molecular weight cut-off of the dialysis bag is 8kD and dialyze for 3 days, change the water 3 times a day) , and then at 25°C, centrifuged at 5000r / min for 10 minutes to obtain a 3.5wt% silk fibroin solution;

[0039] (3) Weigh 0.1g of hyaluronic acid and dissolve it ...

Embodiment 2 3

[0044] Example 2 Three-dimensional porous composite scaffold and preparation method thereof

[0045] The preparation method of the three-dimensional porous composite scaffold of this embodiment comprises the following steps:

[0046] (1) Take 8g silkworm cocoons and place them in a large beaker, add 400ml deionized water, and add 2g anhydrous sodium carbonate at the same time, boil for half an hour, take out, wash with deionized water several times until neutral, then repeat the above steps, place drying in an oven to obtain silk fibroin for use;

[0047] (2), take 1g of silk fibroin and add 25ml of 9.8mol / L lithium bromide solution, after heating in a water bath at 60°C for 3 hours, dialyze with a dialysis bag (the molecular weight cut-off of the dialysis bag is 10kD) for 3 days, change the water 3 times a day, Then at 25°C, centrifuge at 5000r / min for 10 minutes to obtain a 2wt% silk fibroin solution;

[0048] (3) Weigh 0.1g of hyaluronic acid and dissolve it in 1ml of dei...

Embodiment 3 3

[0051] Example 3 Three-dimensional porous composite scaffold and preparation method thereof

[0052] The preparation method of the three-dimensional porous composite scaffold of this embodiment comprises the following steps:

[0053] (1) Take 8g silkworm cocoons and place them in a large beaker, add 400ml deionized water, and add 2g anhydrous sodium carbonate at the same time, boil for half an hour, take out, wash with deionized water several times until neutral, then repeat the above steps, place drying in an oven to obtain silk fibroin for use;

[0054] (2) Add 7.5g silk fibroin to 25ml 9.8mol / L lithium bromide solution, heat it in a water bath at 60°C for 3 hours, then dialyze with a dialysis bag (the molecular weight cut-off of the dialysis bag is 12kD) for 3 days, change the water 3 times a day , and then at 25°C, centrifuged at 5000r / min for 10 minutes to obtain a 10wt% silk fibroin solution;

[0055] (3) Weigh 0.1g of hyaluronic acid and dissolve it in 1ml of deionize...

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Abstract

The invention discloses a three-dimensional porous composite scaffold and a preparation method thereof. The composite scaffold is prepared by taking 2-10 wt% of silk fibroin solution, hyaluronic acid and sodium alginate as raw materials, wherein the ratio of the hyaluronic acid to the silk fibroin solution is 0.1 g : 5-30 ml, the ratio of mixed solution composed of the silk fibroin solution and the hyaluronic acid to sodium alginate is 15-25 ml : 0.1 g; the solution of the hyaluronic acid and sodium alginate is physically mixed with silk fibroin, and the three-dimensional porous composite scaffold can be obtained after freeze-drying. The obtained scaffold is not favorable for adhesion and proliferation of fibroblasts, but also has good hydrophily and the like, some problems of an existing scaffold material are solved, multiple materials are composited, the advantages of original materials are maintained, and property shortcomings of original materials are overcome. The three-dimensional porous composite scaffold is simple in preparation process, the required cost in the needed raw material ratio is low, and the three-dimensional porous composite scaffold is suitable for mass production.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a three-dimensional porous composite scaffold of silk fibroin / hyaluronic acid / sodium alginate and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, mainly divided into the epidermis, dermis and subcutaneous tissue, and has important barrier, mechanical, sensory and regulatory functions for the human body. But the skin is one of the most fragile and vulnerable organs of the human body. Skin ulceration, wounds, surgical injuries and burns are important causes of skin damage, and burns are the main cause of skin damage. As far as China is concerned, there are more than 10 million burn patients every year, of which more than 3 million patients need skin grafts because of severe burns. However, due to the limited source of autologous skin, there will be insufficient skin available when encountering a lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/60
CPCA61L27/48A61L27/56A61L27/60C08L5/08C08L5/04
Inventor 郭瑞蓝咏刘玉
Owner BEOGENE BIOTECH GUANGZHOU
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