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Measuring method for amino acid in electronic cigarette smoke liquid

A technology of e-cigarette liquid and determination method, which is applied in the field of physical and chemical inspection, and achieves the effects of easy promotion, good repeatability and recovery rate, and fast analysis speed

Active Publication Date: 2016-08-17
CHINA TOBACCO GUANGXI IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no public report on the detection method of amino acids in e-cigarette liquid as the research object. In order to accurately grasp the release amount of amino acids in e-cigarette liquid and better control the quality and quality of e-cigarette liquid, the establishment The determination method of amino acids in smoke liquid is of great significance

Method used

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  • Measuring method for amino acid in electronic cigarette smoke liquid
  • Measuring method for amino acid in electronic cigarette smoke liquid
  • Measuring method for amino acid in electronic cigarette smoke liquid

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Experimental program
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Embodiment 1

[0048] Instruments, reagents, and materials: AB SCIEX 5500 LC-MS / MS from the United States; Agilent 1290HPLC from the United States; Agilent ZORBAX Eclipse AAA column (150mm×4.6mm, particle size 5μm), 0.22μm microporous filter membrane, ultrasonic cleaner; ultrapure Water (18MΩ), acetonitrile (chromatographically pure, Bailingwei Chemical Reagent Company), 17 kinds of amino acid mixed standard.

[0049] Preparation of the extract: use concentrated hydrochloric acid to prepare a 0.01mol / L dilute HCl solution as the extract;

[0050] Preparation of internal standard solution: weigh a certain amount of leucine-d 3 , use the extract to prepare an internal standard solution with a concentration of 0.1 μmol / mL;

[0051] Preparation of mixed standard solutions: using the extract as a solvent, mix 17 kinds of amino acids into standard solutions of different concentrations, proline, alanine, isoleucine, leucine, arginine, histidine , methionine, phenylalanine, serine, threonine, tyro...

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Abstract

The invention discloses a measuring method for amino acid in electronic cigarette smoke liquid. The method comprises the steps that a diluted hydrochloric acid solution is used as extraction liquid, an electronic cigarette smoke liquid sample is vibrated and extracted, extraction liquid is filtered through an organic filter film, seventeen kinds of amino acids in the electronic cigarette smoke liquid are measured through a high performance liquid chromatography-tandem mass spectrum combined method, and quantitation is carried out through an internal standard method. By the adoption of isotope dilution internal standard method quantitation, the influences of matrix effects in the analysis process are avoided; direct sampling is adopted, the content of seventeen kinds of amino acids in the electronic cigarette smoke liquid can be rapidly and accurately detected, repeatability and the recovery rate are good, and the measuring method is applicable to rapid analysis of industrial large-batch samples.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing, and in particular relates to a method for determining amino acid content in electronic cigarette liquid. Background technique [0002] Amino acid is the most basic substance that constitutes the protein of the organism, and is one of the indispensable components in the organism. It not only has a variety of physiological functions, but also is widely used in food additives as a taste agent and a source of nutrition. [0003] Nowadays, many e-cigarette liquid research and development units no longer deliberately pursue the taste of traditional cigarettes, but endow them with special tastes or functions. The plant extracts, flavors and fragrances added to e-cigarette liquids are rich in various amino acids. To meet the metabolic needs of the body. However, there is no public report on the detection method of amino acids in e-cigarette liquid as the research object. In order ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 黄善松范忠周芸许蔼飞徐石磊王萍娟周晓严俊潘玉灵唐桂芳
Owner CHINA TOBACCO GUANGXI IND
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