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Preparation method of HPV (human papilloma virus)-specific antibody and freeze-dried preparation of HPV-specific antibody

A specific, HPV-16 technology, used in the preparation of HPV-specific antibodies and the field of freeze-dried preparations, can solve the problems of difficulty in inducing immunity of various vaccines, increase the difficulty of preparation, and increase the cost of vaccines, and achieve low immunogenicity. Sexual, short acting time, fast onset effect

Inactive Publication Date: 2016-08-17
LANJIATANG BIOLOGICAL MEDICINE FUJIAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase of vaccine valence, it not only increases the difficulty of preparation, so that the cost of the vaccine increases sharply, but also brings great challenges to the safety of the vaccine.
In addition, there may be mutual immune interference between multivalent vaccines, making it difficult for each valent vaccine to fully exert its immune-inducing function

Method used

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  • Preparation method of HPV (human papilloma virus)-specific antibody and freeze-dried preparation of HPV-specific antibody
  • Preparation method of HPV (human papilloma virus)-specific antibody and freeze-dried preparation of HPV-specific antibody
  • Preparation method of HPV (human papilloma virus)-specific antibody and freeze-dried preparation of HPV-specific antibody

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] One, a kind of preparation method of the specific antibody (Fab' fragment) of anti-HPV infection

[0024] 1. Gene cloning and protein separation and purification

[0025] 1.1 Query the gene sequence of related protein molecules through NCBI / Genebank, including: HPV (low-risk type, high-risk type) L1, L2 full sequence, heparan sulfate proteoglycan and furin-furin, and obtain the complete gene through gene synthesis sequence.

[0026] Wherein: HPV type-16L1 gene sequence: (SEQ ID NO: 1).

[0027] HPV type-16L2 gene sequence: (SEQ ID NO: 2).

[0028] 1.2 After the L1 and L2 of the above-mentioned heparan sulfate proteoglycans, furin- and HPV-16 subtypes are synthesized by genes, they are cloned into prokaryotic expression vectors or eukaryotic expression vectors (or have SV40 promoter and Eukaryotic enhancers, and appropriate reporter genes such as HRP, fluorescent proteins, etc.). The genetic engineering steps include: designing PCR primers according to the gene seque...

Embodiment 2

[0082] The preparation method of a freeze-dried preparation of anti-HPV infection antibody is as follows: the specific Fab' is mixed with other auxiliary materials and filled. Weigh according to the ratio in Table 1 below.

[0083] Table 1

[0084] raw material name

content

Specific Antibody Fab' Fragments

0.01-0.5mg / ml

polyethylene glycol

0.5-8%

Mannose

5-80%

Trehalose

1-70%

sucrose

0.1-10%

Glycine

0.1-5%

arginine

0.1-5%

Buffered salt (PBS phosphate buffered saline)

0.01-1%

[0085]Inject the purified water of 50% batching volume into the dosing tank, add the mannose, trehalose, sucrose of formula quantity. After stirring to dissolve, add the glycine and arginine in the batching amount, and stir again to dissolve. The phosphate of the batching amount is injected into 20%-30% and dissolved in the batching tank. After the dissolution is complete, it is injected into the main b...

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Abstract

The invention relates to the technical field of biological pharmacy, particularly a preparation method of HPV (human papilloma virus)-specific antibody and a freeze-dried preparation of the HPV-specific antibody. The preparation method comprises the following steps: preparing an antigen, immunizing a chicken with the antigen to obtain an antibody, and carrying out enzymolysis purification to obtain the HPV-specific antibody. The freeze-dried preparation of the anti-HPV-infection antibody is prepared from the following raw materials in parts by weight: 0.5-8 parts of polyethyleneglycol, 5-80 parts of mannitose, 1-70 parts of trehalose, 0.1-10 parts of sucrose, 0.1-5 parts of glycine, 0.1-5 parts of arginine and 0.01-1 part of buffer salt. The freeze-dried preparation also comprises the 0.01-0.5 mg / ml HPV-specific antibody. Compared with the preventive HPV vaccine, the HPV-specific antibody has the advantages of short action time, quick effect taking and the like, and is free from the restriction of the ages.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for preparing an HPV specific antibody and a freeze-dried preparation. Background technique [0002] Human papillomavirus (HPV) mainly causes verrucous lesions of the skin and mucous membranes. According to its relationship with tumorigenesis, HPV can be divided into high-risk types and low-risk types. Among them, high-risk HPV infection has been proved to be the main cause of anogenital cancers including female cervical cancer; low-risk types mainly cause genital warts . The most effective way to prevent and control HPV infection is HPV vaccine, especially the high-risk HPV vaccine that can cause cervical cancer. [0003] At present, the main method to expand the protective scope of vaccines is to increase the valence of vaccines and develop multivalent vaccines. However, with the increase of vaccine valence, it not only increases the difficulty of prepara...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/02C07K1/18C12P21/06A61K9/19A61K39/42A61K47/26A61P31/20
CPCC07K16/084A61K9/19A61K39/00A61K47/26C07K16/02
Inventor 胡博志邹潮兰增金
Owner LANJIATANG BIOLOGICAL MEDICINE FUJIAN CO LTD
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