Novel lactic acid bacterium lactobacillus fermentum isolated from adults in longevity village, helpful for defecation
A new technology of Lactobacillus fermentum, which is applied in the field of probiotics of new Lactobacillus fermentum strains and the above-mentioned strains, to achieve the effects of no antibiotic resistance transfer, improved defecation activity, acid resistance and bile resistance
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[0052] The composition of the present invention can be prepared according to the usual preparation method of probiotic composition, usually, the composition of the present invention can be in the form of culture suspension or dry powder. And, in the effective dose of the above-mentioned Lactobacillus fermentum PL9988 strain or its culture solution as the main component, one or more than two kinds of pharmaceutically acceptable common carriers or one or more than two kinds of additives can be selected. Compositions prepared in usual dosage forms.
[0053]Carriers can be selected from diluents, lubricants, binders, disintegrants, sweeteners, stabilizers, and preservatives. One or more of them can be used. As an additive, it can be selected from flavors, vitamins, and antioxidants. Use one or more than two.
[0054] In the present invention, all pharmaceutically acceptable carriers and additives can be used as carriers or additives, specifically, as diluents, lactose (lactose mo...
Embodiment 1
[0083] Example 1. Isolation of bacterial strains
[0084] Fecal samples of 101 healthy adults with regular and smooth defecation were received from 8 villages in Jeollabuk-do, the so-called longevity belt of Korea, and arrived at the laboratory within 6 hours in a refrigerated state. After diluting the provided stool samples with sterilized saline, they were diluted in DeManRogosa (MRS, Difco, Becton Dickinson, Sparks, MD, USA) culture medium and smeared to incubate in a 37°C incubator for 48 hours. Colonies exhibiting the form of lactic acid bacteria were collected to isolate individual colonies (Lee, HM, LeeY, 2008, Letters in Applied Microbiology 46, 676-681), and when colonies of the same form were observed in the same individual, a maximum of two were analyzed to isolate strains. The isolated above-mentioned strains were screened for negative reaction strains by catalase reaction test, and then Gram staining was performed to isolate both Gram-positive strains and rod-form...
Embodiment 2
[0085] Example 2. Identification of bacterial strains
[0086] In order to identify the strain isolated in Example 1 above, 16S ribosomal ribonucleic acid gene base sequence analysis was performed.
[0087] Specifically, the base sequence uses EzTaxon-eserver ( http: / / eztaxon- e.ezbiocloud.net / ; Kim et al., 2012) and the online BLAST algorithm mat the National Center for Biotechnology Information Webserver ( http: / / www.ncbi.nlm.nih.gov ) to identify the strains, and a molecular phylogenetic analysis based on the 16S ribosomal ribonucleic acid base sequence.
[0088] Ultimately, as figure 1 As shown, it is confirmed as follows: the Lactobacillus fermentum PL9988 strain of the present invention has a 16S ribosomal ribonucleic acid base sequence recorded in sequence 1, and presents 99.42% of the standard bacterial strain CECT562 (Lactobacillus fermentum strain CECT562) of Lactobacillus fermentum Novel strains with homology of 16S ribosomal ribonucleic acid ( figure 1 ). Mo...
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