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Resveratrol embedded peanut protein nano-particles and preparation method thereof

A peanut protein and resveratrol technology, applied in the field of nanoparticles, can solve the problems of generating resveratrol-loaded nanoparticles, exposure of active groups, etc., and achieves the advantages of comprehensive utilization, increased added value, and long sustained release time. Effect

Active Publication Date: 2016-06-29
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the structure of peanut protein is a tight quaternary structure, no active groups are exposed, and it cannot react with resveratrol to generate resveratrol-loaded nanoparticles

Method used

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  • Resveratrol embedded peanut protein nano-particles and preparation method thereof
  • Resveratrol embedded peanut protein nano-particles and preparation method thereof
  • Resveratrol embedded peanut protein nano-particles and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0038] like figure 1 As shown, the preparation method of the described peanut protein nanoparticles embedded in resveratrol comprises the following steps:

[0039] Step A: preparation of peanut protein solution, mixing peanut protein with a certain amount of distilled water.

[0040] Step B: alkali treatment, adjusting the pH of the peanut protein solution to 9-12 with NaOH.

[0041] Step C: heat treatment, heat the peanut protein solution obtained in step B to 50-90° C., and keep it for 20-60 min.

[0042] Step D: Cool the peanut protein solution obtained in step C at room temperature, and adjust its pH to 7 with HCL.

[0043]Step E: preparation of resveratrol solution: mix resveratrol with 95% ethanol solution under light-shielded conditions.

[0044] Step F: Add the resveratrol solution obtained in step E to the peanut protein solution obtained in step D under the condition of avoiding light, and magnetically stir for 1-3 hours to obtain a mixed solution of resveratrol a...

Embodiment 1

[0054] Weigh 1.49 g of peanut protein extracted by alkali-soluble acid precipitation and dissolve it in 250 mL of distilled water to prepare a peanut protein solution with an initial concentration of 5.96 mg / mL, and adjust the pH to 11 with 0.5M NaOH. The above solution was heated in a water bath at 85° C. for 30 min, allowed to stand and cooled to room temperature, and then adjusted to pH 7.0 with HCL to obtain a modified peanut protein solution.

[0055] Dissolve resveratrol in 95% ethanol solution to obtain a resveratrol mass concentration of 0.0348% resveratrol ethanol solution, add 30mL of the aforementioned resveratrol ethanol solution to the modified peanut protein solution, under dark conditions The reaction was stirred for 1.5h. Add 80 mL of CaCl at a concentration of 5.05 mM to the above solution 2 solution, distilled water to 500mL, so that the protein concentration in the final solution was 1 / 2 of the initial protein concentration, and allowed to stand at room tem...

experiment example 1

[0057] The particle size and morphology of the resveratrol-embedded peanut protein nanoparticles prepared in Example 1 were observed with a transmission electron microscope. A certain amount of the prepared resveratrol-embedded solid peanut protein nanoparticles was redissolved in distilled water to prepare a resveratrol-embedded peanut protein nanoparticle solution with a concentration of 1 mg / mL. Take a drop of the above solution and add it to the copper grid covered with polyvinyl formal resin film, place the copper grid horizontally for 2 to 3 minutes to deposit molecular aggregates on the grid surface, absorb the excess solution on the surface with filter paper, and then add 2% of the solution dropwise The hydrogen peroxide acetic acid glaze solution was used to negatively stain the nanoparticles for 2 minutes, and the copper mesh was placed on the filter paper for 3 minutes to fully stain and absorb the excess dye solution. After drying, observe and take pictures with a t...

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Abstract

The invention relates to nano-particles and particularly discloses resveratrol embedded peanut protein nano-particles and a preparation method thereof. According to the nano-particles, resveratrol serves as a core, and modified peanut protein with the quaternary structure opened serves as a shell. According to the preparation method, peanut protein is separated out and extracted from peanut meal by means of alkali solution and acid isolation, the quaternary structure is opened after peanut protein is subjected to alkali modification and thermal modification, active groups are exposed, peanut protein is mixed with resveratrol, peanut protein molecules form small aggregates by means of metal ion induction, and then all the aggregates are promoted to form the resveratrol embedded peanut protein nano-particles through hydrophobic interaction. The peanut meal is used as a raw material, peanut protein in the peanut meal is separated out and extracted and used as a raw material for preparing the resveratrol embedded peanut protein nano-particles, it is beneficial for comprehensive utilization of peanut byproducts, and meanwhile the added value of the peanut meal is increased.

Description

technical field [0001] The present invention relates to nanoparticles, in particular, to resveratrol-encapsulated peanut protein nanoparticles. Background technique [0002] Resveratrol is a biologically strong natural polyphenolic anthraquinone terpene compound, also known as stilbene triphenols, is a chemopreventive agent for tumors, and is also effective in reducing platelet aggregation, preventing and treating atherosclerosis, Chemopreventive agent for cardiovascular and cerebrovascular diseases, with anti-oxidation, anti-tumor, liver protection and other effects. However, its properties are unstable, poor in water solubility, and low in bioavailability. It is prone to oxidation under strong acid and strong alkali, light and high temperature. A carrier of resveratrol, it is especially necessary to encapsulate it to maintain its activity and improve its bioavailability. [0003] In order to improve the stability and bioavailability of resveratrol, the use of nano-system...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K9/50A61K31/05A61K8/02A61K8/64A61K8/34A23L33/105A23P10/30A23J1/14A23J3/14A23J3/22
CPCA23J1/14A23J3/14A23J3/22A23V2002/00A61K8/0225A61K8/347A61K8/645A61K9/5052A61K31/05A61K2800/413A61K2800/56A61K2800/652A23V2200/224A23V2250/082A23V2250/2132A23V2250/548
Inventor 石爱民王强刘红芝陈雪刘丽胡晖杨颖
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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