Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

HPLC method for simultaneously determining contents of four alkaloid components in cortex berberidis

A technology of barberry bark and alkaloids, which is applied in the direction of measuring devices, material separation, and analysis of materials, etc., can solve the hidden dangers of not clearly specifying the endothelium, affecting the quality of medicinal materials, the safety and clinical efficacy of barberry bark, etc. Achieve the effect of promoting development and utilization and improving quality control level

Inactive Publication Date: 2016-06-15
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF7 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, considering that there are many active ingredients in barberry bark, the reliability of using a single active ingredient content as a quality standard is not high.
At the same time, at present, the drug parts of barberry bark are not clearly defined, and the use is more chaotic: "Ministerial Standards Tibetan Medicine Sub-volume" stipulates that it is dry endothelium, but it does not clearly indicate which part of the endothelium; "Standards of Tibetan Medicine" stipulates that it is the inner bark of the stem or root; and market research also found that there are Tibetan medicinal materials market and decoction pieces enterprises still sell barberry bark medicinal materials with cork
The confusion of the drug parts will inevitably affect the quality of the medicinal materials. If you do not know the difference in the content of each active ingredient in different parts of the barberry bark, if you use it in disorder clinically, it will also greatly affect the safety of the barberry bark and the stability of the clinical efficacy. Hidden dangers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC method for simultaneously determining contents of four alkaloid components in cortex berberidis
  • HPLC method for simultaneously determining contents of four alkaloid components in cortex berberidis
  • HPLC method for simultaneously determining contents of four alkaloid components in cortex berberidis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The process parameter screening of embodiment 1 inventive method

[0054] The barberry bark medicinal materials used in this example were selected from the fourth batch: Tibet Autonomous Region Tibetan Medicine Factory.

[0055] 1 Investigation of chromatographic conditions

[0056] (1) Selection of measurement wavelength

[0057] Take magnolialine, jatrorrhizine hydrochloride, palmatine hydrochloride, and berberine hydrochloride reference substance solutions respectively, scan at a wavelength of 200-400nm, and obtain an ultraviolet spectrum (see Figure 1-4 ). According to the ultraviolet absorption spectrum of magnolialine, jatrorrhizine hydrochloride, palmatine hydrochloride, and berberine hydrochloride, take the test solution and measure its peak area at different wavelengths of 230, 260, 270, and 280 nm, and the results are shown in Table 1. . According to the peak areas and response abundances of the four components at different wavelengths, and comprehensively...

Embodiment 2

[0125] Embodiment 2 Methodological verification of the inventive method

[0126] 1 Investigation of linear relationship

[0127] Precisely draw 0.5, 1, 2, 4, 5, 6, and 8 mL of the above-mentioned mixed reference solution, place them in different 10 mL measuring bottles, add methanol to dilute to the mark, and shake well. 10 μL of each of the seven solutions was precisely drawn and injected into the liquid chromatograph for determination. The peak area value of each component is used as the ordinate, and the concentration (mg / mL) is used as the abscissa to perform regression processing to establish a standard curve. The results are shown in Table 12.

[0128] Table 12 Linear relationship inspection results

[0129]

[0130] The results show that each reference substance has a good linear relationship within their respective mass concentration ranges, which proves that the method of the present invention has a wide linear range and high accuracy.

[0131] 2 precision test ...

Embodiment 3

[0151] The HPLC content determination of four kinds of alkaloid components in the barberry bark medical material of embodiment 3

[0152] Get each barberry bark sample, every batch parallel 3 times, carry out HPLC measurement by the method for " 4 " item among the embodiment 1, calculate magnolialine, jatrorrhizine hydrochloride, palmatine hydrochloride and berberine hydrochloride in each sample The results are shown in Table 17.

[0153] The content determination results of four components in the barberry bark medicinal material of table 17 (n=3)

[0154]

[0155] The results showed that the total content of the four active ingredients in the clinically applied and commercially available barberry bark medicinal materials was about 9.51%, among which magnolanine had the highest content (average value 5.98%), followed by berberine (average value 2.43%), The contents of jatrorrhizine (mean value 0.78%) and palmatine (mean value 0.31%) were the lowest.

[0156] Therefore, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Lengthaaaaaaaaaa
Filler particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses an HPLC method for simultaneously determining the contents of four alkaloid components in berberis bark. The four alkaloid components are magnolialine, jatrorrhizine, palmatine and berberine, comprising the following steps: (1) prepare need testing solution; (2) prepare reference substance solution; (3) need test solution and reference substance solution are injected into high-performance liquid chromatograph detection respectively; of magnolialine, jatrorrhizine, palmatine and berberine. The method of the invention is accurate, reliable, simple and quick, and lays a foundation for improving the quality control level of barberry bark and promoting the development and utilization of medicinal materials. Simultaneously, the method of the present invention also accurately detects four alkaloid components in different parts of different gene source varieties of barberry bark, which provides an effective reference for the clinical application of barberry bark bark.

Description

technical field [0001] The invention relates to the technical field of quality control methods for Tibetan medicinal materials, in particular to an HPLC method for simultaneously determining the contents of four alkaloid components in barberry bark. Background technique [0002] The Tibetan medicine barberry bark was first recorded in "Sibu Medical Code", and the Tibetan transliteration "Jilwa", "Jiexing", etc., comes from the dry endothelial bark of Berberis kansuensis Schneid. and several plants of the same genus. Berberis cortex has the effects of clearing away heat and detoxifying, draining yellow water, etc. road infection, eye disease and other diseases, and has good development and utilization value. Therefore, it is of great practical significance to detect the content of active ingredients in barberry bark and monitor the quality of barberry bark. [0003] The active ingredients of barberry bark are mainly alkaloid compounds, such as berberine, magnolialine, palma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 范刚赖先荣李艳
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com