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Fluorescent nanoprobe and its preparation method and method for synchronous detection of multiple hazard factors in food

A fluorescent nanoprobe and synchronous detection technology, which is applied in the field of fluorescent nanoprobe and synchronous detection of various hazard factors in food, can solve the problems of low detection integration, inability to achieve synchronous detection of various hazard factors, weak optical signal, etc. , to achieve the effect of strong fluorescent signal

Inactive Publication Date: 2018-11-20
FOOD INSPECTION CENT OF CIQ SHENZHEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing rare earth fluorescent probes have the problems of weak optical signal and low detection integration, and the existing rare earth fluorescent probes and their supporting detection devices and platforms cannot achieve the purpose of synchronous detection of multiple hazard factors

Method used

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  • Fluorescent nanoprobe and its preparation method and method for synchronous detection of multiple hazard factors in food
  • Fluorescent nanoprobe and its preparation method and method for synchronous detection of multiple hazard factors in food
  • Fluorescent nanoprobe and its preparation method and method for synchronous detection of multiple hazard factors in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] This example gives the preparation method of the fluorescent nanoprobe.

[0058] Specifically: 1. Preparation of chelate precursor

[0059] Solution A: 2.0 mg BBCAP or BHHCT or PTTA was dissolved in 20 μL of 0.05M carbonate buffer (pH 9.5). Solution B: 6.4 mg EDC and 2.0 mg NHS were dissolved in 80 μL absolute ethanol. A and B were mixed and stirred for 20 minutes, 1.5 μLAPTMS was added, and stirred for 2 hours in the dark. Then, add 200 μL of 0.01M EuCl 3 and TbCl 3 The mixture was stirred in the dark for 2 hours to obtain a large number of amino groups and different proportions of Eu 3+ and Tb 3+ Chelates are used as precursors for the synthesis of fluorescent probes with different emission wavelengths.

[0060] 2. Preparation of fluorescent nanoprobes

[0061] A) Synthesis of TEOS nano-cores of fluorescent nanoprobes: 100 μL of the above precursor and 300 μL of ultrapure water were added to 10 mL of reversed-phase micellar system formed by nonionic surfactant ...

Embodiment 2

[0067] This example gives the preparation method of the fluorescent nanoprobe.

[0068] Specifically: 1. Preparation of chelate precursor

[0069] Solution A: 10 mg BBCAP or BHHCT or PTTA was dissolved in 20 μL of 0.05M carbonate buffer (pH 9.5). Solution B: 6.4 mg EDC and 2.0 mg NHS were dissolved in 80 μL absolute ethanol. A and B were mixed and stirred for 20 minutes, 1.5 μLAPTMS was added, and stirred for 2 hours in the dark. Then, add 200 μL of 0.01M EuCl3 and TbCl3 mixture, and stir for 2 hours in the dark to obtain Eu with a large number of amino groups and chelating different proportions. 3+ and Tb 3+ Chelates are used as precursors for the synthesis of fluorescent probes with different emission wavelengths.

[0070] 2. Preparation of fluorescent nanoprobes

[0071] A) Synthesis of TEOS nano-cores of fluorescent nanoprobes: 100 μL of the above precursor and 300 μL of ultrapure water were added to 10 mL of reversed-phase micellar system formed by nonionic surfactan...

Embodiment 3

[0076] A) Synthesis of the TEOS nano-core of the fluorescent nanoprobe: Take 100 μL of the precursor described in Example 1 or Example 2 and 300 μL of ultrapure water and add it to 10 mL of reversed-phase gel formed by the nonionic surfactant TritonX-100 The gelation reaction was carried out in the beam system. The reverse-phase micelle system is mixed according to the volume ratio of TritonX-100, n-hexanol, and cyclohexane in a ratio of 1:1:3, and the reverse-phase micelles are prepared evenly by rapid stirring. Add 100 μL TEOS to the above system, increase the stirring speed, and promote TEOS to enter the “nano pool” in the reversed micelles, then add 50 μL NH 4 OH initiates the hydrolysis reaction. React at room temperature for 24 hours to fully carry out the hydrolysis and condensation reactions. Disperse the collected product in an equal volume of acetone, oscillate ultrasonically for 5 minutes in an ice-water bath, and precipitate by high-speed centrifugation. Disperse ...

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Abstract

The invention discloses a fluorescent nano probe. The fluorescent nano probe comprises a TEOS nano core of a rare earth chelate carried with Eu<3+> and / or Tb<3+>, and the surface bonding with Eu<3+> and / or Tb<3+> in the rare earth chelate of the TEOS nano core is modified with a layer of TEOS shell layer. The invention provides the fluorescent nano probe with strong optical signal and used for detecting harmful factors in food with high flux, at the same time, the invention also provides a preparation method of the fluorescent nano probe, and also provides a method for synchronously detecting a plurality of harmful factors in food by employing the fluorescent nano probe.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a fluorescent nanoprobe, a preparation method of the fluorescent nanoprobe, and a method for synchronous detection of multiple hazard factors in food. Background technique [0002] The detection of hazard factors in food has always been an important topic in the field of food safety, but the current method that can monitor the common hazard factors in dairy products sensitively, stably, reliably, quickly, easily, and at low cost can achieve a one-step "full-scale" detection of high-frequency hazard factors in dairy products. Detection technology is still lacking. [0003] For example, the common "high-frequency hazard factors" in dairy products, such as melamine, aflatoxin M1, and Beta-lactam antibiotics, mainly include physical and chemical detection based on chromatography-mass spectrometry, immunoassay detection based on the principle of antigen-antibody reaction, and based on th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/533
CPCG01N33/533G01N33/543
Inventor 张恒易长青林燕奎刘慧玲
Owner FOOD INSPECTION CENT OF CIQ SHENZHEN
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