Genetic engineering biological indicator
A biological indicator and genetic engineering technology, applied in the field of disinfection and sterilization biological indicators, can solve the problem of inconsistency between enzyme heat resistance and spore heat resistance.
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Embodiment 1
[0033] The genetic engineering biological indicator is composed of the following parts: Bacteria sheet, which is evenly distributed with genetic engineering indicator microorganisms.
[0034] The inner packaging ensures that the bacteria slices are in a sterile environment during storage, and at the same time, the effective sterilizing factor can fully contact the bacteria slices during the sterilization process.
[0035] The ampoule bottle is sealed with 2ml soybean peptone liquid medium and 100mM IPTG inducer. The soybean peptone liquid medium and IPTG inducer are separated from the bacteria sheet during the sterilization process and allowed to contact with the bacteria sheet after the sterilization.
[0036] The genetic engineering indicator microorganism contains: 1*10 6 cfu~5*10 6 cfu Bacillus stearothermophilus spores, the exogenous gene is transfected into Bacillus stearothermophilus by pUB110 plasmid. The exogenous genes include the ampicillin resistance gene, locate...
Embodiment 2
[0038]The genetic engineering biological indicator is composed of the following parts: Bacteria sheet, which is evenly distributed with genetic engineering indicator microorganisms.
[0039] The inner packaging ensures that the bacteria slices are in a sterile environment during storage, and at the same time, the effective sterilizing factor can fully contact the bacteria slices during the sterilization process.
[0040] The ampoule bottle is sealed with 2ml nutrient broth medium and 100mM indole acrylic acid inducer inside, the nutrient broth medium and indole acrylic acid inducer are separated from the bacteria sheet during the sterilization process, allowing the same bacteria sheet contact.
[0041] The genetic engineering indicator microorganism contains: 1*10 6 cfu~5*10 6 cfu Bacillus subtilis spores, the exogenous gene is transfected into Bacillus subtilis by pSBPTQ plasmid. The exogenous genes include the LacZ blue-white screening gene, which is located downstream of...
Embodiment 3
[0043] The genetic engineering biological indicator is composed of the following parts: bacteria sheet, which is evenly distributed with genetic engineering indicator microorganisms.
[0044] The inner packaging ensures that the bacteria slices are in a sterile environment during storage, and at the same time, the effective sterilizing factor can fully contact the bacteria slices during the sterilization process.
[0045] The ampoule bottle is sealed with 0.5% glucose broth medium and arabinose inducer in its inner seal. The 0.5% glucose broth medium and arabinose inducer are separated from the bacteria sheet during the sterilization process and allowed to contact with bacteria.
[0046] The genetic engineering indicator microorganism contains: 1*10 6 cfu~5*10 6 cfu Bacillus subtilis black mutated spores, the exogenous gene is transfected into the subtilis black mutated spores by pIH41 plasmid. The exogenous genes include the neomycin resistance gene, which is located downs...
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