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Quality evaluation method for dendrobe medicinal material

A technology of quality evaluation and dendrobium, which is applied in the directions of measuring devices, material separation, and analysis of materials, etc., can solve the problems of one-sided evaluation methods, indicating the overall quality of Dendrobium medicinal materials, etc., and achieve the effect of complete preservation, simple pretreatment method, and good stability

Active Publication Date: 2016-03-23
深圳市兰标科技有限公司
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Problems solved by technology

[0003] The purpose of the present invention is to provide a quality evaluation method of Dendrobium medicinal materials, to solve the problem that the quality evaluation of Dendrobium medicinal materials is mainly limited to the quality evaluation of individual or certain types of components, and its evaluation method is relatively one-sided, cannot be repeated, and is not systematic and complete enough. technical issues indicating the overall quality of Dendrobium medicinal materials

Method used

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  • Quality evaluation method for dendrobe medicinal material
  • Quality evaluation method for dendrobe medicinal material
  • Quality evaluation method for dendrobe medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Dendrobium candidum quality evaluation method

[0047] Instruments and reagents: Instruments: Agilent1200series high performance liquid chromatography (including low-pressure binary gradient pump G1312A, column thermostat G1316A, diode array detector G1315B, Chemstation chemical workstation, American Agilent Technology Co., Ltd.), 100g portable pulverizer (Guangzhou Xulang Machinery Equipment Co., Ltd.) Milli-QSynthesis ultrapure water purification system (Millipore Company), RQ-250B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), PB303-N electronic balance (MEETTLERTOLEDO, 0.001g), 0.45μm Microporous filter head (Jinteng Company). Reagents: acetonitrile, methanol (chromatographically pure, Fisherscientific, USA); other reagents are analytically pure; ultrapure water (MilliQ ultrapure water, passed through a 0.45 μm microporous membrane); acetic acid (Beijing Dongfang Fine Chemicals Co., Ltd.).

[0048] The chromatographic conditions for the...

Embodiment 2

[0062] Embodiment two: the quality evaluation method of Dendrobium drumsticks

[0063] The difference from Example 1 is:

[0064] Preparation of standard sample solution: take different batches of dry samples of Dendrobium chrysalis, pulverize them with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder, place it in a 100ml Erlenmeyer flask, add 50mL75% Methanol (V water: V methanol = 25:75), after ultrasonication for 30 minutes at room temperature, it was taken out, filtered, the filtrate was concentrated to dryness by rotary evaporation, dissolved in 75% methanol solvent (V water: V methanol = 25:75), and finally transferred to Dilute to volume in a 5ml volumetric flask, shake well, and filter with a 0.45μm microporous membrane to obtain standard sample solutions of different batches.

[0065] Table 310 batches of Dendrobium drumstick fingerprint similarity evaluation results

[0066]

[0067] According to t...

Embodiment 3

[0069] Embodiment three: the quality evaluation method of Dendrobium dendrobii

[0070] The difference from Example 1 is:

[0071] Preparation of standard sample solution: take different batches of dry samples of Dendrobium nobile, pulverize them with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder, place it in a 100ml Erlenmeyer flask, add 50mL75% Methanol (V water: V methanol = 25:75), after ultrasonication at room temperature for 30 minutes, it was taken out, filtered, the filtrate was concentrated to dryness by rotary evaporation, dissolved in 75% methanol solvent (V water: V methanol = 25:75), and finally transferred to Dilute to volume in a 5ml volumetric flask, shake well, and filter with a 0.45μm microporous membrane to obtain standard sample solutions of different batches.

[0072] Table 410 batches of dendrobium dendrobium fingerprint similarity evaluation results

[0073]

[0074] According to th...

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Abstract

The invention relates to a quality evaluation method for a dendrobe medicinal material. The method includes the steps that a schaftoside chromatographic peak is adopted as a reference substance, and a standard fingerprint spectrum is formulated through HPLC (high performance liquid chromatography); through comparison of fingerprint spectrums of samples for test and the standard fingerprint spectrum, the quality of the samples for test is determined. According to the method, the pretreatment method for all the samples for test is simple, characteristic components are reserved, and the solution stability of the samples for test is good. The high performance liquid chromatography is high in precision, good in reproducibility and short in analysis time, and has certain specificity, the separation effect of all fingerprint peaks in the obtained fingerprint spectrum is good, the quality of the dendrobe medicinal material can be accurately and comprehensively reflected, the quality standard of the dendrobe medicinal material is further perfected, and therefore the quality evaluation method can be applied to quality grading and authenticity of dendrobe tissue culture seedlings, fresh dendrobe and air-dried dendrobe of medicinal dendrobe.

Description

technical field [0001] The invention relates to the quality analysis of medicinal materials in the field of traditional Chinese medicines, in particular to a method for evaluating the quality of various dendrobium medicinal materials by HPLC (high performance liquid chromatography) fingerprinting. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium candidum, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which 76 are found in my country. Dendrobium has a long history of medicinal value. As early as in ancient times, Dendrobium was listed as a nourishing top grade in "Shen Nong's Materia Medica". For a long time, with the development of the times, Dendrobium has been regarded as a precious Chinese herbal medicine with very important nourishing...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 梁琼麟邵自星梁晓萍赵田
Owner 深圳市兰标科技有限公司
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