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Paper chip gravity / capillary flow chemiluminescence method

A paper chip and gravity technology, applied in the field of microfluidic analysis, can solve the problems of large volume, large size of device system, high price, etc., and achieve the effects of good biocompatibility, simple processing method and good hydrophilicity

Inactive Publication Date: 2016-03-02
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, these reported flow chemiluminescence methods for microfluidic chips still have some disadvantages: (1) Almost all chips are based on substrate materials such as glass, silicon, polymers, etc. The process is relatively complicated; (2) The microchannel required for the reaction is long and the design of the micromixer is meticulous and precise, which increases the difficulty of the chip microfabrication process; (3) The size of the entire device system is still large, and the PMT optical detection system often used in the system (4) Most flow chemiluminescence microfluidic chip systems require external pumps for liquid manipulation, and some external pipelines are required to connect chip channels and pumps.
However, so far, no report on the detection of heavy metals or heavy metal ions by chemiluminescence has been found on paper chips.

Method used

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  • Paper chip gravity / capillary flow chemiluminescence method
  • Paper chip gravity / capillary flow chemiluminescence method
  • Paper chip gravity / capillary flow chemiluminescence method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A method for preparing a paper chip driven by gravity / capillary force coupling, comprising the steps of:

[0070] (a) Using Adobe Illustrator CS5 software to design the pattern of the hydrophilic region and the hydrophobic region of the paper chip ( figure 2 ). The hydrophilic area on the pattern includes the sample application area [4mm (length) × 5mm (width)], the fluid channel area [10, 15 or 20mm (length) × 3, 4 or 5mm (width)] and the detection area Then, based on these patterns, a 300-mesh mesh screen is processed by Guangzhou Lianchang Printing Equipment Store.

[0071] (b) Whatman No. 1 chromatography paper (200mm × 200mm) was cut into several pieces of paper with a size of 60mm × 90mm, and then the screen was attached to the paper; brushed on the screen with a purple crayon, and further used Grind evenly with a smooth grinding spoon; after grinding, place the paper and the screen together on a heating plate (model YH-946B) set at 85°C for about 5 seconds (t...

Embodiment 2

[0077] The application of the paper chip GCF-CL of embodiment 1 in detecting Cr(III) comprises the following steps:

[0078] (a) Put the plastic bracket assembled with the paper chip into the dark box, align the detection area of ​​the paper chip with the macro lens (model M1614-MP) of the CCD camera (model MC15), adjust the CCD related parameters (such as exposure time, blue light Gain, etc.) and focal length to make the image clearest.

[0079] (b) 5-50 μL of test solution containing Cr(III) was added dropwise to the center of the detection area of ​​the paper chip, and then 10-45 μL of chemiluminescence primer was dropped into the sample area of ​​the paper chip to trigger the GCF-CL reaction .

[0080] (c) Using CCD camera video imaging GCF-CL process, the video imaging parameters are 15 frames per second, the bit rate is 3000, and through VGIF ( http: / / video-to-gif.watermark-software.com / ) software processes the imaging video during the period from the closure of the ...

Embodiment 2

[0088] Several important factors (pH value, component concentration of the test solution and bottom solution) that affect the luminous intensity of the paper chip GCF-CL in Example 2 are optimized

[0089] a) Preferred EDTA concentration

[0090] 1. 30 μL test liquid composition: Cr(III) concentration of 50 μg / mL, KBr concentration of 0.75 M, EDTA concentration to be determined, pH 3.0; 30 μL bottom liquid composition: Luminol concentration of 9 mM, H 2 o 2 The concentration is 0.4M, pH11.5.

[0091] 2. Set up several groups of experiments: the concentration of EDTA in the GCF-CL test solution is set to several different values ​​(0mM, 1mM, 5mM, 10mM, 15mM, 20mM, 25mM).

[0092] 3. The paper chip GCF-CL detection process is the same as in Example 2, and the test results are as follows Figure 11 shown.

[0093] It can be seen from the experimental results that when the EDTA concentration is greater than 10mM, the chemiluminescence intensity is inhibited to a certain extent...

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Abstract

The present invention discloses a paper chip gravity / capillary flow chemiluminescence method, and specifically discloses a paper chip for driving fluid by coupled gravity / capillary force, and a preparation method and application thereof in detection of heavy metal ions. The paper chip of the invention is divided into a hydrophobic region and a hydrophilic region; and the hydrophilic region is divided into a loading zone, a detection zone and a fluid passage zone. For usage, the paper chip is closely placed on a stand, and the slanted surface of the stand and the horizontal plane form an angle; and after placement, the loading zone and the fluid passage zone are on an inclined surface of the stand, and the detection zone is on the horizontal portion of the stand. Compared with the conventional flow chemiluminescence, the paper chip and detection method do not use any expensive pumping unit (precision syringe pump, peristaltic pump, etc.) to drive the liquid flow. The method only uses the natural gravity of liquid and capillary force of paper fiber voids to drive the flow of liquid.

Description

technical field [0001] The invention belongs to the field of microfluidic analysis, and relates to a paper chip gravity / capillary flow chemiluminescence method, in particular to a paper chip with gravity / capillary force coupling driving fluid, a preparation method thereof and an application in detecting heavy metal ions. Background technique [0002] At present, Cr(III), Cr(VI), Co(III), Hg(II), Mn(VII), Cd(II), Pb(II), Ni(II), Te(III) and other heavy metal ions The frequent occurrence of pollution incidents has caused great harm to human health and the natural environment. Therefore, it is very important to realize the rapid detection of heavy metal ions in dealing with major emergencies or emergency incidents. [0003] In recent years, chemiluminescence has developed quite maturely as a detection method, and is widely used in the analysis of substances such as heavy metal ions, nucleic acids and proteins. Chemiluminescence detection is mainly divided into static system, f...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 章春笋张鹏
Owner SOUTH CHINA NORMAL UNIVERSITY
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