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Preparation method of bionic porous tissue-engineered cartilage scaffold

A porous tissue and engineering technology, applied in the field of biomedicine, can solve problems such as compound ratio transformation, achieve the effect of improving cartilage quality and good application prospects

Active Publication Date: 2016-01-13
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Wu Hong and others have used human umbilical cord Wharton'sjelly to construct tissue engineering scaffolds, which changed the physical structure of human umbilical cord Wharton'sjelly, but did not carry out compound ratio modification according to the characteristics of cartilage extracellular matrix components

Method used

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  • Preparation method of bionic porous tissue-engineered cartilage scaffold
  • Preparation method of bionic porous tissue-engineered cartilage scaffold

Examples

Experimental program
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preparation example Construction

[0024] Such as figure 1 As shown, a kind of biomimetic porous tissue engineering cartilage scaffold preparation method of the embodiment of the present invention comprises the following steps:

[0025] S101: separate and prepare human umbilical cord Wharton's jelly, measure it, store it at low temperature, and crush it into a suspension;

[0026] S102: Prepare decellularized Wharton's gum suspension through repeated hypotonic freezing-thawing and enzymatic digestion;

[0027] S103: Mix the decellularized Wharton gum suspension with chondroitin sulfate in different proportions, add EDAC again and mix thoroughly, place at room temperature at a distance of 5-10 cm from the light source, and cross-link under the irradiation of 258nm wavelength ultraviolet light;

[0028] S104: Place the above cross-linked scaffolds in a -20°C refrigerator and a -80°C low-temperature refrigerator successively to shape the scaffolds, vacuum freeze-dry them in a vacuum freeze dryer to obtain shaped...

Embodiment 1

[0031] Step 1, separate and prepare human umbilical cord Wharton gum, measure it, store it at low temperature, and crush it into a suspension;

[0032] Step 2, preparing decellularized Wharton gum suspension through repeated hypotonic freeze-thaw and enzyme digestion;

[0033] Step 3: Mix the decellularized Wharton’s gum suspension with chondroitin sulfate, Wharton’s gum suspension: chondroitin sulfate = 2:0.25, add EDAC again and mix well, place at room temperature 5-10cm away from the light source Cross-linking under 258nm wavelength ultraviolet light irradiation;

[0034] Step 4: Place the above cross-linked scaffolds in a -20°C refrigerator and a -80°C low-temperature refrigerator successively to shape the scaffolds, vacuum freeze-dry them in a vacuum freeze dryer to obtain shaped scaffolds, store and seal them in sealed bags, and irradiate at 60°C Sterilize and store at 4°C for later use.

Embodiment 2

[0036] Step 1, separate and prepare human umbilical cord Wharton gum, measure it, store it at low temperature, and crush it into a suspension;

[0037]Step 2, preparing decellularized Wharton gum suspension through repeated hypotonic freeze-thaw and enzyme digestion;

[0038] Step 3, mix the decellularized Wharton’s gum suspension with different proportions of chondroitin sulfate, the mass ratio of Wharton’s gum suspension to chondroitin sulfate is 2.0:0.75; add EDAC again and mix well, place at room temperature Cross-linking under the irradiation of 258nm wavelength ultraviolet light at a distance of 5-10cm from the light source;

[0039] Step 4: Place the above cross-linked scaffolds in a -20°C refrigerator and a -80°C low-temperature refrigerator successively to shape the scaffolds, vacuum freeze-dry them in a vacuum freeze dryer to obtain shaped scaffolds, store and seal them in sealed bags, and irradiate at 60°C Sterilize and store at 4°C for later use.

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Abstract

The invention discloses a preparation method of a bionic porous tissue-engineered cartilage scaffold. The preparation method comprises the following steps: mixing a decellularized Whartons jelly suspension and chondroitin sulfate at the mixing ratio of the Whartons jelly suspension to chondroitin sulfate being 2.0:0.25-2.0:2.0, adding ethyl dimethylamine propyl carbide dimethylamine and fully uniformly mixing, and crosslinking at room temperature under the UV-irradiation of 258 nm wavelength 5-10 cm from a light source; putting a cross-linked scaffold into a cryogenic refrigerator to make the scaffold to be shaped, carrying out vacuum freeze drying to obtain a molded scaffold, storing the molded scaffold in a hermetic bag and sealing, disinfecting under irradiation at 60 DEG C, and preserving at 4 DEG C for standby. The prepared cartilage scaffold has bionic and porous characteristics, can induce stem cells in vitro to be differentiated to chondrocytes to promote expression of cartilage matrix components proteoglycan and collagen type II, can be used for in vitro construction of tissue-engineered cartilago articularis, and provides a high-quality scaffold material for repairing articular cartilage defects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing a bionic porous tissue engineering cartilage support. Background technique [0002] Articular cartilage defects caused by various reasons such as trauma and infection are very common in clinic. Since articular cartilage has no blood supply, innervation and lymphatic circulation, its self-repair ability is poor. Once the area of ​​cartilage damage is large (>2.0cm), it is basically unable to repair itself, and it can even accelerate the degeneration of articular cartilage, resulting in joint dysfunction and affecting the quality of life of patients. Although the current treatment methods have many methods, such as microfracture, cartilage mosaic plastic surgery, subchondral bone drilling, etc., the long-term effect is not ideal, and it is difficult to restore hyaline articular cartilage. [0003] Since the concept of "tissue engineering" ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/50
Inventor 亓建洪宋洪强陈彬陈超谢地李文华周路
Owner TAISHAN MEDICAL UNIV
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