Aspergillus flavus strain incapable of producing aflatoxin and application of aspergillus flavus strain in biological prevention and control of aflatoxin pollution of peanuts

A technology of aflatoxin and Aspergillus flavus strains, applied in the field of environmental biology, can solve problems such as limited production and application, and achieve the effects of reducing aflatoxin pollution, inhibiting infection, and good colonization ability

Active Publication Date: 2016-01-06
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But generally speaking, the biological control of peanut aflatoxin pollution is still very limited due to various factors, and it is almost still a blank in our country.

Method used

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  • Aspergillus flavus strain incapable of producing aflatoxin and application of aspergillus flavus strain in biological prevention and control of aflatoxin pollution of peanuts
  • Aspergillus flavus strain incapable of producing aflatoxin and application of aspergillus flavus strain in biological prevention and control of aflatoxin pollution of peanuts
  • Aspergillus flavus strain incapable of producing aflatoxin and application of aspergillus flavus strain in biological prevention and control of aflatoxin pollution of peanuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation, screening and identification of strains

[0023] (1) Surface sterilization of peanut kernels with 70% ethanol for 30s, followed by soaking the kernels with 1wt% NaClO for 2 min, washing with sterile water 3 times, drying the peanut kernels, and evenly arranging the peanut kernels in a sterile plastic petri dish Medium, 10 grains / dish, keep the space between the kernels, not close to each other; after wrapping the petri dish containing the seeds with parafilm, carefully place it in the bottom layer of the finishing box with added moisture, cover the box, 30 ℃ culture for 7d;

[0024] (2) Use sterilized tweezers to pick up a small amount of yellow-green conidia produced on the kernels, fully suspend and serially dilute them in Tween-20 containing 0.1 wt%, and take 10 -4 Diluted conidia suspension was coated on PDA plate, cultured at 30 °C for 2 days, and a small amount of the growing single colony was transferred to the center of fresh CYA and AFPA ...

Embodiment 2

[0032] Example 2: Application of Aspergillus flavus NAFFHN396 in biological control of peanut aflatoxin contamination

[0033] The non-toxic Aspergillus flavus NAFFHN396 and the toxigenic Aspergillus flavus AF2202 were cultured on PDA solid medium for 7 days at 30°C, 0.1wt% Tween-20 water was used to suspend conidia, and the number of conidia was counted on a hemocytometer. , adjust the concentration of non-toxin-producing bacteria and toxin-producing bacteria to 1*10 4 , the ratio is 1:1, mixed in equal amounts and inoculated in the center of the CYA solid medium, or inoculated on the surface of the surface sterilized peanut kernels, shake the kernels sufficiently to make the conidia evenly adhere to the surface of the kernels, and at the same time CYA solid medium inoculated with AF2202 alone or peanut kernels was set up as a control, and cultured at 30°C for 7 days. The method described in step (4) of Example 1 was used to extract aflatoxin on the CYA solid medium; the ext...

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Abstract

The invention belongs to the field of the environmental biotechnology, and specifically relates to an aspergillus flavus strain incapable of producing aflatoxin and an application of the aspergillus flavus strain in biological prevention and control of aflatoxin pollution of peanuts. The aspergillus flavus strain incapable of producing aflatoxin is named as aspergillus flavus NAFFHN396, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 28, 2015, and the preservation serial number is CGMCC No: 10927. The aspergillus flavus strain incapable of producing aflatoxin with deletion of an aflatoxin synthetic cluster gene is obtained, and has an obvious inhibiting effect for the yield of the toxin of the strain with strong capacity of producing aflatoxin, thus a biocontrol bacterium source is provided for the biological prevention and control of the toxic aspergillus flavus generated in the field in the future, the aspergillus flavus strain is separated from China, has good colonization capacity in peanuts and potential field competitive advantage, and has great significances in inhibiting infection of the peanuts by the toxin production aspergillus flavus, and reducing the pollution of the aflatoxin in the peanuts.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and particularly relates to a strain of Aspergillus flavus that does not produce aflatoxin and its application in biological control of peanut aflatoxin pollution. Background technique [0002] Aflatoxin is a very important mycotoxin that endangers food safety in my country. In nature, some crops, including corn and peanuts, are susceptible to Aspergillus flavus, resulting in aflatoxin contamination of grains; other plants, such as nuts, are also susceptible to aflatoxin contamination. Aflatoxins are highly toxic and are the most toxic carcinogenic mycotoxins known so far. They are extremely toxic to the liver and central nervous system of humans and animals. Long-term low-dose intake can lead to embryonic malformation, mutation, Gene and chromosomal mutations can induce primary liver cancer, gastric cancer and other cancers, and a large amount of intake at one time can cause acute...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/67
Inventor 晏立英廖伯寿雷永万丽云康彦平
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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