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Penicillum oxalicum, and applications thereof

A technology of Penicillium oxalate and citrus, applied in the application, fungicides, fungi and other directions, can solve the problems of high cost of refrigeration and modified atmosphere preservation, unstable quality, and damage to mature fruits in the growing period.

Active Publication Date: 2015-12-23
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly harms the ripe fruit in the growth period and the fruit in the storage period, which seriously affects the development of the citrus industry.
[0003] Traditionally commonly used citrus fresh-keeping methods include refrigeration, modified atmosphere and chemical preservation methods. However, refrigeration and modified atmosphere preservation have high costs, high energy consumption, and unstable quality. They have been gradually eliminated, and chemical preservation methods have potential food safety and environmental pollution problems. And long-term use of chemical agents to keep fresh, citrus pathogenic bacteria will develop resistance to them

Method used

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  • Penicillum oxalicum, and applications thereof
  • Penicillum oxalicum, and applications thereof
  • Penicillum oxalicum, and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Embodiment one: the separation and preservation of Penicillum oxalicum (Penicillumoxalicum) SG4

[0017] In 2012, the roots of Cypress cypress branches were collected in Yanzhiba, Yichang, Hubei, in the Three Gorges Basin. After surface disinfection, they were inoculated on PDA solid medium with a working concentration of 25 μg / ml penicillin to isolate fungi.

[0018] Surface disinfection: Rinse the root of Cypress cypress branch with tap water for 5 times, dry it with sterile filter paper, soak it in 75% alcohol for 1 min, rinse it with sterile water for 3 times, then soak it with 3% sodium hypochlorite for 60 seconds, and then rinse it with sterile water 5 times, wash dry root surface moisture with sterile filter paper.

[0019] Cultivation: Use sterile scissors to cut the sterilized root into 0.5*0.5cm size, inoculate it into 25 μg / ml penicillin PDA solid medium (potato 200g / L, glucose 20g / L, agar 20g / L, Ph6.0-6.5), cultured at 28°C.

[0020] Strain purification: A...

Embodiment 2

[0023] Embodiment two: Penicillum oxalicum (Penicillumoxalicum) SG4 identification

[0024] 18SrDNA sequence analysis: 18SrDNA sequence was amplified by colony PCR using universal primers. Upstream primer ITS1: 5'-TCCGTAGGTGAACCTGCG-3', downstream primer ITS4: 5'-TCCTCCGCTTATTGATATGC-3'. The reaction system is as follows:

[0025]

[0026] Reaction conditions: pre-denaturation at 94°C for 5min, denaturation at 94°C for 1min, annealing at 56°C for 30sec, extension at 72°C for 1.min, 33 cycles, and finally extension at 72°C for 10min. The PCR product was detected by 1% agarose gel electrophoresis, and a band of about 750 bp was picked for gel recovery and sent to Shanghai Sangon for sequencing.

[0027] Submit the measured sequence to GenBank and obtain the accession number KR024319. The sequence was compared by Blast in GenBank, and the sequence of the strain with higher homology was selected to construct the 18S rDNA phylogenetic tree with MEGA5 software.

[0028] Embod...

Embodiment 4

[0030] Example 4: Antagonistic effect of Penicillum oxalicum SG4 cells on Penicillium citrus.

[0031] Preparation of agar blocks: Inoculate the endophytic fungus strains of S. annulus on the PDA medium by streaking on a plate, culture them at 28°C for 4 days, and use a puncher (diameter 10 mm) to inoculate the agar covered with the test bacteria. Punch holes in the plates for the preparation of mushroom cakes. Test the antibacterial activity of bacteria against Penicillium citrus by plate confrontation method: Inoculate Penicillium citrinum on PDA slant medium, culture at 28°C for 4 days, add 9ml of sterile water to the test tube, and scrape off all spores with an inoculation loop to obtain For the spore suspension, get 500 μl of Penicillium citrus spore suspension, spread it evenly, place the endophytic fungus cake of Shuicypress spp. ), and the other area was placed in reverse (bacteria face down), cultured at 28°C for 4 days, and the results were observed.

[0032] Antib...

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Abstract

The invention discloses a Penicillum oxalicum, and applications thereof. The preservation name of the Penicillum oxalicum is Penicillum oxalicum SG4, and is preserved on 30th, April, 2015, in China Center For Type Culture Collection, in Wuhan university, Wuhan, China; and preservation number is CCTCCM 2015270. It is confirmed by experiments that the Penicillum oxalicum SG4 possesses strong inhibition effects on orange Penicillium citrinum, and can be used for preparing orange biological fresh-keeping agents; a novel direction is provided for development of novel biological fresh-keeping agents and orange biological preservation; and excellent economic and social benefits are achieved.

Description

technical field [0001] The invention relates to a strain of Penicillium oxalicum and its application, which is mainly used in the biological preservation of citrus. The Penicillium oxalicum can be used to antagonize the Penicillium citrus. Background technique [0002] Penicillium citrus is mainly caused by Penicillium italicum. At the initial stage of the disease, the peel softens and fades in a water-soaked state. Afterwards, aerial mycelium grows on the lesion, forming a thick layer of white mold, and rapidly expands into citrus. White subcircular mildew spots. It mainly harms the ripe fruit in the growth period and the fruit in the storage period, seriously affecting the development of the citrus industry. [0003] Traditionally commonly used citrus fresh-keeping methods include refrigeration, modified atmosphere and chemical preservation methods. However, refrigeration and modified atmosphere preservation have high costs, high energy consumption, and unstable quality. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A23B7/155C12R1/80
Inventor 刘士平蒋维秦王阁阁秦田明薛艳红唐皓舒德华
Owner CHINA THREE GORGES UNIV
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