Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method for multiple amino acids in food

A detection method and amino acid technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems that the detection data cannot fully represent the protein content of the product and cannot be distinguished, and achieve the effect of strong versatility and avoiding interference.

Active Publication Date: 2015-11-25
北京市产品质量监督检验院
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many disadvantages in this method. The test data cannot fully represent the protein content of the product, and it is impossible to distinguish whether it is the protein content in the product or artificially added high-nitrogen substances, such as melamine.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method for multiple amino acids in food
  • Detection method for multiple amino acids in food
  • Detection method for multiple amino acids in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0069] (1) Standard solution preparation

[0070] Select a mixed standard solution of 31 kinds of amino acids, which are: taurine, urea, aspartic acid, hydroxyproline, threonine, serine, asparagine, glutamic acid, sarcosine, Glycine, Alanine, Citrulline, Alpha-Aminobutyric Acid, Valine, Cystine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Beta-Alanine , β-aminobutyric acid, γ-aminobutyric acid, tryptophan, ethanolamine, hydroxylysine, ammonia, lysine, histidine, carnosine, arginine; concentration of amino acid mixed standard solution: 0.25μmol / mL (Pickering, calibration standard); successively dilute with eluent into five gradients: 0.005 μmol / mL, 0.0125 μmol / mL, 0.025 μmol / mL, 0.05 μmol / mL, 0.25 μmol / mL.

[0071] (2) Measurement conditions

[0072] (1) Chromatographic conditions:

[0073] A. The chromatographic column adopts:

[0074] Pre-column: cation-exchange lithium guard column, inner diameter 2.0×20mm, 5 μm (purchased from Pickering Laboratories Catal...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection method for a plurality of amino acids in food and application of ninhydrin post-column derivatization-high performance liquid chromatography in detection of a plurality of amino acids in wines. The detection method is characterized in that a high performance liquid chromatograph and an amino acid post-column derivatization apparatus are used to detect a plurality of amino acids in food under specific chromatographic conditions and post-column derivatization conditions. The method provided by the invention can detect more than 30 amino acids in food at the same time, has good versatility and is applicable to detection of amino acids in different foodstuffs. Moreover, derivative reaction takes place after separation of amino acids from other substances, so interference by other substances is avoided, and the method is applicable to analysis of a plurality of amino acids in an unknown complex sample.

Description

technical field [0001] The invention relates to a method for detecting multiple amino acids in food and the application of ninhydrin post-column derivatization-high performance liquid chromatography in detecting multiple amino acids in wine. Background technique [0002] In recent years, China's food safety issues have emerged frequently. In the "artificial honey" incident in 2007, some criminals added thickeners, sweeteners, preservatives, flavors and pigments and other chemicals to fake honey. The fake honey had almost no nutritional value; the illegal addition of industrial raw materials in 2008: "Sudan red duck eggs", "melamine milk". The "melamine" incident was a food safety incident that shocked the world. In the immediate storm of industry purges, the public had to accept the fact that quality problems caused by milk sources generally existed in the entire industry, and the problems of large enterprises and well-known brands were particularly concentrated. , China's...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 吴颖
Owner 北京市产品质量监督检验院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com