Compound feed additive
A compound feed and additive technology, applied in animal feed, animal feed, application, etc., can solve the problems of low pig absorption rate, high excretion rate, and environmental pollution, and achieve good compatibility, low excretion rate, and small environmental pollution Effect
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Embodiment 1
[0033] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance
[0034] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and culture. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator.
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Embodiment 2
[0051] Example 2: (Comparative Test) Fermentation Conditions for High-yielding Carotenoids of Rhodotorula marina WYN1
[0052] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , the inoculum amount was 10%, the shaker speed was 180r / min, and after culturing at 28°C for 24 hours, the temperature was adjusted to 30°C, the pH was adjusted to 5, and the nitrogen source was added once, that is, peptone was added according to the addition amount of 2.5%, and continued Ferment for 24h. The marine Rhodotorula WYN1 was fermented and cultured by the above method, and its biomass, carotenoid production and intracellular copper content were 27.52 g / L, 25.12 mg / L and 6240 μg / g dry bacteria, respectively.
Embodiment 3
[0053] Embodiment 3: feed additive and its preparation
[0054] The parts by weight of the feed additive are composed as follows:
[0055] 10 compound polysaccharide extracts, 15 sea red yeast fermented cultures, 6 poplar flower extracts, 5 yams, 5 centella asiatica, 2 scallions, and ebony plums; the sea red yeast preservation number is CCTCCNO: M2014592.
[0056] The preparation method of described complex polysaccharide extract is as follows:
[0057] The raw materials astragalus, alfalfa and wolfberry were dried and crushed through a 60-mesh sieve at a mass ratio of 4:2:1, and boiling water was added to 8 times the total weight of the crushed matter, extracted twice for 30 minutes each time, filtered, mixed with the extracts, and cooled naturally to At room temperature, add absolute ethanol to the extract solution to make the ethanol concentration in the mixture reach 70%, seal and refrigerate for precipitation at 10°C for 8 hours, dry the precipitate in vacuum, crush it t...
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