Bacterial cellulose porous foamed material and preparation method thereof

Abstract

The invention relates to a bacterial cellulose porous foamed material and a preparation method thereof. Pores in the foamed material exist in bacterial cellulose hydrogel, are dense and in large quantities and have a uniform size. The preparation method comprises the following steps: (1) preparing the nanometer bacterial cellulose hydrogel; (2) soaking the bacterial cellulose hydrogel in a hydrogen peroxide or acid solution; (3) heating the hydrogen peroxide solution in which the bacterial cellulose hydrogel is soaked, adding a strongly oxidizing agent or adding a catalyst at room temperature, and carrying out uniform reaction; or adding excess carbonate into the acid solution in which the bacterial cellulose hydrogel is soaked, and carrying out uniform reaction; (4) carrying out cleaning with water; and (5) carrying out shock cooling or gradual cooling and then carrying out freeze drying so as to obtain the bacterial cellulose porous foamed material. The bacterial cellulose porous foamed material has great pores suitable for growth of cells, a high specific surface area and appropriate porosity. Experiment results show that cells grow well in the pores. The bacterial cellulose porous foamed material has application potential in the aspect of a scaffold for tissue engineering.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose materials, in particular to a bacterial cellulose porous foam material and a preparation method thereof. Background technique [0002] Bacterial cellulose is mainly a kind of polymer with nanofiber network structure fermented by acetic acid bacteria such as Acetobacter xylinum. The bacterial cellulose synthesized by different strains had little difference in microstructure and mechanical properties. Since Brown first reported bacterial synthesis of cellulose in 1886, countries around the world have carried out a lot of research on this kind of cellulose. After a series of studies, it is known that bacterial cellulose is a straight chain formed by β-D-glucose through β-1,4-glycosidic bonds. The straight chains are parallel to each other, not in a helical conformation, and without branched structure. In its three-dimensional structure, hydrogen bonds are tightly cross-linked between the same lin...

AI Technical Summary

Problems solved by technology

Chinese patent CN201310224291 and patent CN201310110593 disclose the preparation method of bacterial cellulose three-dimensional display porous scaffold, all are to pretreat paraffin wax by three-dimensional rapid prototyping technology, and inoculate seed liquid into paraffin wax to carry out fermentation culture, culture 6-14 days later will The paraffin was removed to obtain a three-microarray porous scaffold, which took a long time to prepare a porous scaffold

The foaming agent and other materials used in the reaction will cause certain pollution to the environment

[0004] Chinese patent CN200810053552 introduces a preparation method of a porous bacterial cellulose sponge, by mixing a plurality of bacterial cellulose hydrogel membranes with an ice crystal porogen evenly, pre-freezing at -20~-50°C and making a concentration It is a homogeneous dispersion system of 0.1-1%, and the porous bacterial cellulose sponge is prepared by phase separation method, and the mechanical and physical properties of the bacterial cellulose film are greatly reduced by freeze-drying during the reaction process.

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