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Tumor phototherapy reagent based on cell membrane coating, as well as preparation method and application of tumor phototherapy reagent

A technology of cell membranes and reagents, applied in the field of anti-tumor phototherapy reagents, can solve the problems of weakening the yield of singlet oxygen and phototherapy efficiency, and achieve the improvement of yield of singlet oxygen and fluorescence brightness, inhibition of self-quenching, good biophase capacitive effect

Active Publication Date: 2015-09-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the π-π stacking interaction between porphyrin molecules, it is easy to form aggregates in aqueous solution, and this self-quenching phenomenon greatly weakens the yield of singlet oxygen and the efficiency of phototherapy.

Method used

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  • Tumor phototherapy reagent based on cell membrane coating, as well as preparation method and application of tumor phototherapy reagent
  • Tumor phototherapy reagent based on cell membrane coating, as well as preparation method and application of tumor phototherapy reagent
  • Tumor phototherapy reagent based on cell membrane coating, as well as preparation method and application of tumor phototherapy reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Firstly, using glycol chitosan (glycol chitosan) and N-hydroxysuccinimide ester-polyethylene glycol 2000-methoxyl (NHS-PEG2000-OMe) as raw materials, synthesize PEG molecules to account for chitosan The percentage of sugar repeating units is 20% of the precursor, ie chitosan-20% PEG. Weigh 40mg of NHS-PEG2000-OMe and 20mg of ethylene glycol chitosan respectively, dissolve them in PBS buffer (pH 7.4, 50mM), mix quickly and uniformly, and react overnight at room temperature in a shaker. After the reaction, it was purified by dialysis with a dialysis bag with a molecular weight cut-off of 10k for 3 days, and finally freeze-dried in a lyophilizer to make chitosan-20% PEG.

[0042] (2) Subsequently, chitosan-20% PEG, protoporphyrin (PpIX), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinyl Imine (NHS) was used as a raw material to synthesize a phototherapy agent in which PpIX molecules accounted for 4% of chitosan repeating units: chitosan-20%PEG-...

Embodiment 2

[0046] According to the method of Example 1, protoporphyrin is replaced by indocyanine green, adjust the consumption of each component, the percentage of synthetic PEG accounting for chitosan repeating unit number is 2%, and indocyanine green molecule accounts for chitosan repeating unit The percentage of the number is 0.2% of the reagent.

Embodiment 3

[0048] According to the method of Example 1, protoporphyrin is replaced by chlorin E6, the consumption of each component is adjusted, the percentage of synthetic PEG accounting for chitosan repeating unit number is 30%, and chlorin E6 molecule accounts for chitosan The percentage of repeating units is 2% of reagents.

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Abstract

The invention discloses a tumor phototherapy reagent based on cell membrane coating. The tumor phototherapy reagent is high molecular weight chitosan of which the side chain contains a photosensitizer molecule and a polyethylene glycol molecule. The invention further discloses a preparation method of the tumor phototherapy reagent and application of the tumor phototherapy reagent to preparation of a photosensitizer. Compared with the prior art, the reagent has the advantages that the toxicity to cells is very low under the condition of no irradiating, and under the irradiation of light with certain wavelength, the photodynamics toxicity is improved greatly when being compared with that of free porphyrin molecules. Above all, the self quenching effect between porphyrin molecules is solved effectively, and the cell membrane imaging and photodynamics tumor therapy based on the porphyrin molecules are achieved.

Description

technical field [0001] The invention relates to a tumor phototherapy reagent based on cell membrane coating and its preparation method and application, belonging to the field of anti-tumor phototherapy reagents. Background technique [0002] Cancer is a disease caused by the abnormal control of cell growth mechanism. It may appear in various tissues in the human body and invade the surrounding normal tissues to metastasize, becoming an important factor that endangers human life and health. Traditional cancer treatment methods include surgical resection, chemotherapy and radiation therapy, etc., but the resulting secondary damage is often greater than the cancer itself, so these treatment methods still need to be improved. Photodynamic therapy is a new type of adjuvant cancer therapy developed in recent years. It has attracted extensive attention due to its advantages of less damage to normal tissues, no accumulation of drug toxicity, and less side effects. Among them, the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/48A61K49/00A61P35/00G01N21/64G01N1/30A61K47/61
Inventor 吴富根贾浩然
Owner SOUTHEAST UNIV
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