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Fast preparation method of biological affinity copper nanometer cluster

A technology of copper nanoclusters and physical properties, which is applied in the field of nanomaterial preparation, can solve the problems of low quantum yield of copper nanoclusters, poor light stability, and long synthesis time, and achieve light stability, good biophilicity, and improved stability Effect

Inactive Publication Date: 2015-07-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a biophilic copper nanocluster for the existing method for synthesizing copper nanoclusters with long synthesis time, high cost, low quantum yield and poor light stability of the synthesized copper nanoclusters The rapid preparation method and its application to Hg in water samples 2+ Content detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Mix 20mmol / L copper sulfate solution and 20mg / mL bovine serum albumin solution at a volume ratio of 1:5, adjust the pH of the solution to 12 with 1.0mol / L sodium hydroxide solution, and stir at room temperature for 5min to obtain purple copper - bovine serum albumin complex; dropwise add 0.1mol / L of H at a speed of 20D / min 2 o 2 15mL, incubated at 55°C for 1h to obtain a light yellow copper nanocluster solution, which was stored in a refrigerator at 4°C; pipette 600μL of the prepared copper nanocluster solution into a centrifuge tube, and add 100μL of Hg 2+ Shake well, incubate at room temperature for 10 min, and then test the fluorescence intensity on a fluorescence spectrophotometer with excitation wavelength and emission wavelength of 320nm and 420nm, respectively. Calculate Hg according to the fluorescence emission peak intensity and substitute the linear regression equation 2+ content, resulting in Hg 2+ The detection limit was 4.7×10 -12 mol / L. The relative s...

Embodiment 2

[0031] Mix 20mmol / L copper nitrate solution and 15mg / mL bovine serum albumin solution at a volume ratio of 1:5, adjust the pH of the solution to 12 with 1.0mol / L potassium hydroxide solution, and stir at room temperature for 3 minutes to obtain purple copper - bovine serum albumin complex; add 1.0 mol / L of H dropwise at a speed of 10D / min 2 o 2 1.5 mL, incubated at 55°C for 1 hour to obtain a light yellow copper nanocluster solution, which was stored in a refrigerator at 4°C; pipette 600 μL of the prepared copper nanocluster solution into a centrifuge tube, and add 100 μL of Hg 2+ Shake well, incubate at room temperature for 20 minutes, and then test the fluorescence intensity on a fluorescence spectrophotometer with excitation and emission wavelengths of 320nm and 420nm, respectively. Calculate Hg according to the fluorescence emission peak intensity and substitute the linear regression equation 2+ content, resulting in Hg 2+ The detection limit was 5.2×10 -12 mol / L. The...

Embodiment 3

[0033] Mix 20mmol / L copper chloride solution and 50mg / mL bovine serum albumin solution at a volume ratio of 2:3, adjust the pH of the solution to 12 with 1.0mol / L potassium hydroxide solution, and stir at room temperature for 3min to obtain purple Copper-bovine serum albumin complex; drop 0.1mol / L of H at a speed of 20D / min 2 o 2 20mL, incubated at 70°C for 0.5h to obtain a light yellow copper nanocluster solution, which was stored in a refrigerator at 4°C; pipette 800μL of the prepared copper nanocluster solution, and add 200μL of Hg 2+ Put the standard solution or sample solution in a centrifuge tube, shake well, incubate at room temperature for 30 minutes, and then test the fluorescence intensity on a fluorescence spectrophotometer with excitation wavelength and emission wavelength of 340nm and 428nm, respectively. Calculate Hg according to the fluorescence emission peak intensity and substitute the linear regression equation 2+ content, resulting in Hg 2+ The limit of d...

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Abstract

The invention relates to a fast preparation method of a biological affinity copper nanometer cluster, is applied to the detection of Hg<2+> content in a water sample, and belongs to the technical field of nanometer material preparation. A copper source solution and a protein solution are uniformly mixed; the pH of the solution is regulated to the alkalinity by alkali; oxidizing agents are dropped; heating incubation is carried out for a period of time; the copper nanometer cluster is prepared; the copper nanometer cluster is used for detecting the fluorescence intensity of an Hg<2+> standard solution or an Hg<2+> sample solution; the fluorescence intensity is substituted into a linear regression equation, and the Hg<2+> content in the sample is calculated. Due to strong oxidizing property and coordinating capability of oxidizing agents, the secondary structure in protein can be changed, copper-protein-oxidant complexes can be formed, the reducing capacity of the protein is enhanced, and the formation of the copper nanometer cluster is obviously accelerated. The copper nanometer cluster is used for the Hg<2+> detection in the water sample, and the method is obviously superior to the prior art in the aspects of analysis time, sensitivity, selectivity and cost.

Description

technical field [0001] The invention relates to a rapid preparation method of biophilic copper nanoclusters and its application to Hg in water samples 2+ The detection of content belongs to the technical field of nanometer material preparation. Background technique [0002] Metal nanoclusters are new nanomaterials composed of tens to hundreds of atoms. Different from metal atoms, metal nanoparticles and metal simple substances, the size of metal nanoclusters is close to the Fermi wavelength of electrons, which can generate discontinuous electronic energy levels and exhibit unique optical, electrical and chemical properties (Díez I. ; Ras R.H.A. Springer Ser. Fluoresc. 2010, 9:307-332). Compared with semiconductor quantum dots and organic fluorescent dyes, metal nanoclusters not only produce size-dependent and tunable fluorescence, but also have many other excellent properties. For example, large Stokes shift, high fluorescence quantum efficiency and good biocompatibility ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 李在均廖小晴贝红霞
Owner JIANGNAN UNIV
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