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Method for determining content of effective ingredient amygdalin in loquat leaf cough syrup

A technology for cough loquat syrup and amygdalin, which is applied in the field of drug detection, can solve problems such as unsuitable preparation content indicators, and achieve good quality, good repeatability, high stability and high accuracy.

Inactive Publication Date: 2015-07-15
HUBEI DUANZHENG PHARMA CO LTD
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  • Abstract
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Problems solved by technology

[0004] The 2010 edition of the Chinese Pharmacopoeia uses ursolic acid and oleanolic acid as the content determination components of loquat leaves. However, due to the loss caused by the preparation process, the content of ursolic acid and oleanolic acid is very small, so ursolic acid and oleanolic acid Fruit acid is not suitable as the content index of this preparation

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  • Method for determining content of effective ingredient amygdalin in loquat leaf cough syrup
  • Method for determining content of effective ingredient amygdalin in loquat leaf cough syrup
  • Method for determining content of effective ingredient amygdalin in loquat leaf cough syrup

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Embodiment

[0020] The invention provides a method for measuring the content of active ingredient amygdalin in cough loquat syrup, comprising the following steps:

[0021] (1) Preparation of the reference substance solution: take an appropriate amount of the reference substance amygdalin, accurately weighed, add mobile phase to make a solution containing 80 μg per 1 mL, and obtain the reference substance amygdalin solution;

[0022] (2) Preparation of the test solution: Measure 5mL of this product cough syrup, put it in a 10mL volumetric flask, add ethanol to make it up to the scale of the volumetric flask, shake well, filter with filter paper, take 1mL of the filtrate, put it in a 10mL volumetric flask, and use Dilute the mobile phase to the scale of the volumetric flask, shake well, filter with a 0.45 μm filter membrane, and take the subsequent filtrate, which is the test solution;

[0023] (3) Determination by high-performance liquid chromatography: octadecylsilane bonded silica gel is...

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Abstract

The invention discloses a method for determining content of effective ingredient amygdalin in loquat leaf cough syrup. The method comprises a step of preparation of reference solution, a step of preparation of test solution and a step of high performance liquid chromatographic determination, wherein the condition that content of amygdalin is not less than 1.2mg per 1ml of the loquat leaf cough syrup can be ensured. According to the method, high performance liquid chromatography is used for determining content of the amygdalin controlling quality of loquat leaf cough syrup product, compared with the detection without content determination item in the existing standard, the method has the advantages that the inherent quality of medicine can be directly controlled by determining content of the amygdalin, moreover, in the determination method, the amygdalin is separated well, stability and accuracy both are high and repeatability is excellent, thus, quality of the loquat leaf cough syrup product can be controlled better.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a method for measuring the content of the active ingredient amygdalin in cough-relieving loquat syrup. Background technique [0002] Antitussive loquat syrup is a commonly used traditional Chinese medicine preparation, which has the effects of clearing the lung, relieving cough, and reducing phlegm. It is used for the treatment of cough with phlegm and bronchitis. Booklet) (WS3-B-1702-94) only stipulates routine inspections, specifically: (1) Take 1mL of cough loquat syrup of this product, add 10mL of water, shake vigorously, and persistent foam will form; (2) Take this product 10mL, add sodium hydroxide test solution to make it alkaline, extract with 10mL chloroform, separate the chloroform layer, extract with 1% sulfuric acid solution, add bismuth potassium iodide test solution to the acid solution, and form a brown precipitate. This standard does not have content determination items...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 余国斌
Owner HUBEI DUANZHENG PHARMA CO LTD
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