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Rapid myoglobin detection method and corresponding detection kit

A myoglobin and detection method technology, which is applied in the field of myoglobin rapid detection method and corresponding detection kit, immunoassay, can solve the problems of low detection sensitivity and poor precision, and achieve good repeatability and consistency. Good sex, no-rinse effect

Inactive Publication Date: 2015-05-06
FENGHUI SHANGHAI MEDICAL SCI& TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The common detection methods of Myo in the market are immune projection turbidimetry, immune scattering turbidimetry, and colloidal gold immunochromatography. In recent years, some manufacturers have introduced some more advanced detection methods. The existing methods have detection sensitivity. Low, poor precision and other limitations

Method used

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  • Rapid myoglobin detection method and corresponding detection kit
  • Rapid myoglobin detection method and corresponding detection kit
  • Rapid myoglobin detection method and corresponding detection kit

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Experimental program
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Embodiment Construction

[0033] In order to understand the technical content of the present invention more clearly, the specific implementation methods of the present invention will be further described below.

[0034] The principle of the detection method of the present embodiment is as Figure 1~2 As shown, the curve of singlet oxygen attenuation with particle distance is as follows image 3 shown.

[0035] Raw materials, reagents, and instrument sources used in the following examples are as follows:

[0036]

[0037]

[0038] NaHCO 3 The formula of the solution:

[0039] raw material name

Standard dosage (1L)

sodium bicarbonate

8.410g

purified water

Dilute to 1000ml

[0040] CB (pH9.6) buffer (0.05M)

[0041] raw material name

Standard dosage (1L)

Sodium carbonate

1.540g

sodium bicarbonate

2.940g

[0042] purified water

Dilute to 1000ml

[0043] Recipe for Tris buffer:

[0044]...

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Abstract

The invention relates to a rapid myoglobin detection method which is characterized by comprising the following steps: (1) mixing nanoscale photosensitive microspheres which are internally filled with dye, luminous microspheres enveloped with active molecules, active molecules marked by biotin, and a sample to be detected to obtain a mixture; (2) carrying out incubation culture on the mixture, and enabling all components with immunological characteristics to have reaction to obtain a compound; and (3) irradiating the compound by laser to enable the compound to emit light, and measuring by a photon counter to obtain the detection value. The invention also provided a rapid myoglobin detection kit. The rapid myoglobin detection method and the corresponding detection kit are stable in detection results, high in accuracy rate, low in cost, high in sensitivity, good in precision, wide in detection range and suitable for clinical popularization.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to immunoassay, in particular to a rapid detection method for myoglobin and a corresponding detection kit. Background technique [0002] Myoglobin (Myo) is an oxygen-binding heme protein that is mainly distributed in cardiac and skeletal muscle tissues. In acute myocardial injury, Myo is first released into the blood. About 2-3 hours after the onset of symptoms, Myo in the blood can exceed the normal upper limit, reach the peak at 9-12 hours, and return to normal after 24-36 hours. Serial sampling is recommended for patients with suspected ACS because of the delay between the onset of symptoms and the release of protein markers into the blood. The clinical utility of early diagnosis and monitoring of ACS has been extensively reported in the literature. Negative Myo helps rule out myocardial infarction. [0003] Myo has a wide range of clinical applications. Its value ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N33/542
CPCG01N33/6887G01N2333/805G01N2800/325
Inventor 张伟卫君超葛望舒郑东
Owner FENGHUI SHANGHAI MEDICAL SCI& TECH
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