C-reactive protein quick-detecting method and C-reactive protein quick-detecting kit

Abstract

The invention relates to a C-reactive protein quick-detecting method which is characterized by comprising the following steps: (1) mixing nanometer photosensitive microspheres with dye inside, luminous microspheres coated with active molecules, active molecules labeled with biotin and a to-be-detected sample to obtain a mixture; (2) performing incubation cultivation on the mixture, and performing reaction on components with immunological characteristics to obtain a conjugate; and (3) irradiating the conjugate with laser to enable the conjugate to shine, and detecting with a photon counter to obtain a detection number value. The invention further provides a C-reactive protein quick-detecting kit. The C-reactive protein quick-detecting method and the C-reactive protein quick-detecting kit provided by the invention have the advantages of stable detection result, high accuracy rate, low cost, high sensitivity, high precision and wide detection range and are suitable for clinical popularization.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to immunoassay, specifically a C-reactive protein rapid detection method and a detection kit. Background technique [0002] Human C-reactive protein (C-reactive protein, CRP) refers to some proteins (acute proteins) that rise sharply in plasma when the body is infected or tissue damaged. CRP can activate complement and strengthen the phagocytosis of phagocytes to play an opsonizing role, thereby removing pathogenic microorganisms invading the body and damaged, necrotic, and apoptotic tissue cells, and playing an important protective role in the body's natural immune process. The research on CRP has a history of more than 70 years. The traditional view is that CRP is a non-specific inflammatory marker, but research in the past ten years has revealed that CRP is directly involved in inflammation and cardiovascular diseases such as atherosclerosis, and is The most powerful ...

AI Technical Summary

Problems solved by technology

However, due to the relatively backward detection methods of CRP in the past, high false positives and false negatives have affected its clinical value. In recent years, due to the update of detection technology, a fast, simple and reliable method for measuring CRP has been established rapidly.

[0012] The common detection method of CRP in the market is immunoturbidimetry. In recent years, some manufacturers have introduced some more advanced detection methods. The existing methods have limitations such as low detection sensitivity and poor precision.

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