Method for simultaneously measuring plasma concentration of amoxicillin and clavulanate potassium
A technology of potassium amoxicillin-clavulanate and potassium clavulanate, applied in the field of simultaneous determination of blood concentration of potassium amoxicillin-clavulanate, can solve the problems of many interference factors, expensive instruments, low sensitivity, etc., and achieve specificity Strong, simple method, good reproducibility
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[0017] Simultaneously measure the method for amoxicillin-clavulanate potassium blood drug concentration, comprise the following steps:
[0018] (1) Prepare amoxicillin sodium-clavulanate potassium standard stock solution: containing 88.3 micrograms / ml of amoxicillin sodium and 45.3 micrograms / ml of potassium clavulanate;
[0019] (2) Prepare acetaminophen internal standard solution and potassium dihydrogen phosphate buffer;
[0020] (3) Sample pretreatment: Take amoxicillin sodium-clavulanate potassium standard stock solution in a centrifuge tube, add blank plasma to 215 microliters, accurately add 10 microliters of internal standard acetaminophen, vortex for 10 seconds, and then Precisely add 200 microliters of methanol, vortex for 15s, centrifuge at 4000r / min for 6min at 4°C, and take the supernatant for later use;
[0021] (4) Take the supernatant from step (3) and enter the chromatographic column as C18, use acetonitrile-0.05mol / L potassium dihydrogen phosphate buffer 10:...
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