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Method for comprehensively utilizing useful substances in shrimp and crab skin

A useful technology for shrimp and crab, which is applied in the field of comprehensive utilization of useful substances in shrimp and crab skin, can solve problems such as low safety, environmental impact, and imperfect process technology, and achieve the effect of increasing production income

Active Publication Date: 2014-11-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although there are many processing techniques for shrimp and crab shells in China, most of them have the defects that the process technology is not perfect and the product value is not high.
For example, in the patent application number CN02122565.6, a method for producing chitin, astaxanthin and protein with fresh shrimp shells, the use of chloroform extraction can only obtain a mixture containing astaxanthin and fat, and the product safety is low. industrial use
The dilute alkaline solution used in the treatment of proteins will also cause environmental pollution
The application number is CN200910037545.9, an environmental protection method patent for comprehensive preparation of biologically active substances from shrimp shells. Cyclohexane reagent is used to extract the crude fat of shrimp and crab skin, which also has low safety and the product is easy to contain pigments. And the application of high-concentration sodium hydroxide and hydrochloric acid when extracting protein and deacetylating chitin will have a huge impact on the environment
The application number is CN200510050390.4 A method patent for producing chitin, astaxanthin, protein, calcium powder and biological fertilizers from shrimp shells. The biological fertilizer components are only production waste plus phytic acid and active oligosaccharides, which cannot fully provide nutrients needed for plant growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 illustrates that the present invention comprehensively utilizes the method for useful substance in shrimp and crab skin

[0023] Soak the shrimp and crab skin in boiling water, dry at room temperature, mechanically crush and pass through a 5-mesh sieve, take 1 kg of the pretreated shrimp and crab skin and put it into a 50 L bioreactor, and then add 20 L of food-grade citric acid with a mass percentage of 25% , for 10 min ultrasonic treatment, ultrasonic power 100 w, ultrasonic temperature 20°C, after the end of ultrasonication, keep the temperature at 40°C and stir at 50 rpm, separate the extract after 2 h of processing time, and vacuum dry The citric acid-calcium citrate flavored calcium supplement can be obtained by processing, and the calcium ion extraction rate is 76.3%. Wash the treated shrimp and crab skin to neutrality, add 0.1 L of ALCALASE 2.4 L alkaline protease (protein concentration is 100 mg / mL), turn on the circulating water to keep the enzyma...

Embodiment 2

[0024] Embodiment 2 illustrates that the present invention comprehensively utilizes the method for useful substance in shrimp and crab skin

[0025] Soak shrimp and crab skin in boiling water, dry at room temperature, mechanically crush and pass through a 100-mesh sieve, weigh 1 Kg of the pretreated shrimp and crab skin and add it to a 50 L bioreactor, then add 25% by mass of food-grade citric acid 30 L, turn on the stirring. The reactor was placed in an ultrasonic breaker for ultrasonic treatment for 30 min, ultrasonic power: 600 w, ultrasonic temperature 40 °C, after the end of the ultrasonic wave, stir at a constant temperature of 40 °C at a speed of 100 rpm, and separate the extract after 6 h of treatment time. Separate the extract, and vacuum-dry it to get citric acid-calcium citrate flavored calcium supplement, and the extraction rate of calcium ions can reach 86%. The leaching filter residue was washed with water until neutral, 4 g of papain (600 U / mg) was added, and ...

Embodiment 3

[0026] Embodiment 3 illustrates that the present invention comprehensively utilizes the method for useful substance in shrimp and crab skin

[0027]Soak shrimp and crab skin in boiling water, dry at room temperature, mechanically crush and pass through a 50-mesh sieve, weigh 1 Kg of pretreated shrimp and crab skin and add it to a 50 L bioreactor, then add 25% by mass of food-grade malic acid and Add 5 L of acetic acid and start stirring. The reactor was placed in an ultrasonic breaker for ultrasonic treatment for 90 min, ultrasonic power: 100 w, ultrasonic temperature 60 ° C, and stirring was continued after ultrasonic treatment, and the reaction time was 24 h. Separate the extract, and vacuum dry it to get citric acid-calcium citrate flavored calcium supplement, and the extraction rate of calcium ions can reach 79%. The leached filter residue was washed with water until neutral, 4 g of papain (600 U / mg) was added, the circulating water was turned on to maintain the enzymolys...

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Abstract

The invention relates to a method for comprehensively utilizing useful substances in shrimp and crab skin. The method is characterized by mainly comprising the steps that first, mineral substances, protein, pigment and chitin in the shrimp and crab skin are recycled; second, the chitin is degraded with an enzymatic method to prepare N-acetyl-D-glucosamine; third, enzymolysis waste residues are applied. Due to the method, the useful substances in the shrimp and crab skin are extracted and recycled step by step, raw materials can be fully utilized, the problem that leftovers of products in the shrimp and crab industry are contaminated is solved, and resources are saved to the maximum extent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for comprehensively utilizing useful substances in shrimp and crab skin. Background technique [0002] N-acetyl-D-glucosamine is the basic unit of many important polysaccharides in biological cells, especially in the exoskeleton of marine crustaceans. N-acetyl-D-glucosamine and its derivatives are widely used. In clinical medicine, it is currently the drug for treating rheumatism and rheumatoid arthritis. In addition, it also has the function of enhancing the human immune system. By inhibiting cancer The overgrowth of cells or fibroblasts can inhibit and treat cancer and malignant tumors. In the food industry, it can also be used as food antioxidants, food additives for infants and sweeteners for diabetics. [0003] my country is rich in shrimp and crab resources, mainly distributed in the middle and lower reaches of the Yangtze River. Jiangsu is a key provinc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C05G3/00C12P19/26A23L1/304A23L1/30A23K1/00A23K10/26A23L33/10A23L33/16
Inventor 陈可泉高聪何珣张阿磊欧阳平凯
Owner NANJING UNIV OF TECH
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