Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hepatitis B virus surface l protein related peptide

A protein and virus technology, applied in the direction of viral peptides, viruses, antiviral agents, etc.

Active Publication Date: 2017-04-19
SHANGHAI HEP PHARMA
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. Current status of prevention and treatment of HBV infection
In addition, other adverse reactions such as antigen-antibody complex immunopathology may also occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hepatitis B virus surface l protein related peptide
  • Hepatitis B virus surface l protein related peptide
  • Hepatitis B virus surface l protein related peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1: Inhibition of HBV hepatocyte infection by peptides derived from HBV surface L protein of B genotype adw serotype and C genotype adr serotype

[0086] 1. Preparation of peptides derived from surface L protein of HBV serotype B genotype adw serotype and C genotype adr serotype

[0087] N-terminal myristoyl-modified HBV surface L protein-derived peptides of B genotype adw serotype and C genotype adr serotype (SEQ ID NO: 3 amino acid glycine modified with myristoyl, expressed as Myr 13-59) The AB 431A peptide synthesizer follows the standard Fmoc protocol and uses 0.25mM HMP as the starting resin to extend the synthesis residue by residue from the carboxyl end to the amino end. To enhance the stability of the polypeptide, the C-terminus of the polypeptide was further amidated. After peptide synthesis, cut with cutting solution, filter out resin with G6 glass sand funnel, and vacuum dry the filtrate. Ion-free water dissolves peptide cleavage products, Explore...

Embodiment 2

[0098] Example Two: Inhibition of HBV Hepatocyte Infection by Peptides Derived from Surface L Protein of D Genotype Ayw Serotype HBV Virus

[0099] 1. The synthesis of peptide Myr 2-47 (SEQ ID NO: 9) derived from surface L protein of HBV serotype D ayw serotype D with myristoyl modification and the culture of primary human hepatocytes are the same as in Example 1.

[0100] 2. Cultivation of D genotype ayw serotype HBV virus

[0101] The 2.2.15 cell line, which can secrete complete D genotype ayw serotype HBV infected virus particles, was continuously cultured for 10 days. The culture supernatant was collected, centrifuged with 6% polyethylene glycol (PEG8000) to precipitate virus particles, the pellet was resuspended in phosphate buffer containing 25% fetal calf serum, and frozen at -80°C.

[0102] 3. Detection of HBsAg in hepatocyte culture supernatant after HBV infection (same as embodiment one)

[0103] 4. Inhibition of HBV hepatocyte infection by HBV surface L protein-de...

Embodiment 3

[0105] Example 3: Screening of HBV surface L protein derivatized peptides

[0106] 1, B genotype adw serotype and C genotype adr serotype HBV virus genotype serotype determination, virus culture, cultivation of human primary hepatocytes (same example 1).

[0107] 2. The construction of a recombinant plasmid carrying a complete HBV genome and having the complete replication ability of HBV.

[0108] Add 310μl proteinase K lysate to 50μl HBV virus culture suspension [1mg / ml proteinase K, 50mmol / L Tris-HCl (pH8.0), 200mmol / L NaCl, 10mmol / L EDTA, 2% SDS, 1μg / ml poly(A )]. After lysis at 60°C for 1 hour, extract with phenol / chloroform, precipitate with ethanol, and dissolve the precipitate in H 2 O is HBV DNA solution. Entire EN II replication element was amplified with upstream primer (Pst I) 5'ctgactgcagCACTGGATGGGGCTTGGCTATTGG (SEQ ID NO:21, 1202-1225) and downstream primer (EcoRI) 5'ttatggaattcCGACGCGGCGATTGAGACCTTC (2201-2180, SEQ ID NO:22) C genotype adr serotype HBV genom...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to hepatitis B virus (HBV) surface L protein derived peptide which is used for preventing and curing HBV infection, a screening method which is used for removing polypeptide antigenicity and antigenicity-removing derived peptide which is screened and obtained through the screening method. An HBV surface L protein pre-S1 area has a key amino acid sequence of virus adherent cell surface receptors and an antigenicity amino acid sequence which is combined with antibody against L protein. Polypeptide in the HBV surface L protein pre-S1 area can inhibit the infection of the HBV on cells. The invention also provides the screening method which is used for removing polypeptide antigenicity. The antigenicity-removing derived peptide is screened, can inhibit HBV infection and does not combine with the antibody.; Therefore, the mutual interference between natural L protein derived peptide and the antibody against the L protein during HBV infection inhibition is avoided, and both the antigenicity-removing derived peptide and the antibody against the HBV surface L protein can inhibit the HBV infection.

Description

[0001] The patent application of the present invention is a divisional application of the Chinese invention patent application with the application number 201210177978.6, which is a divisional application of the invention patent application filed on August 12, 2005 and whose invention name is "hepatitis B virus surface L protein-related peptide". case. technical field [0002] The present invention relates to HBV surface L protein-derived peptides used for preventing and treating hepatitis B virus (human hepatitis B virus, HBV) infection, a screening method for removing the antigenicity of these polypeptides, and the de-antigenic derived peptides obtained by screening through the method. In particular, the present invention relates to L protein pre-S1 region polypeptides derived from HBV of B genotype adw serotype, C genotype adr serotype and D genotype ayw serotype HBV. Also in particular, the present invention relates to a screening method for removing the antigenicity of th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/02A61K39/29A61K38/16A61P1/16A61P31/20
CPCA61K38/00C07K14/005C12N2730/10122C12N2730/10133
Inventor 刘宏利汪裕尹颖
Owner SHANGHAI HEP PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com