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Preparation method of guinea pig airway allergic asthma model

A technology for allergic asthma and guinea pigs, applied in the field of biomedicine, to achieve good repeatability, good sensitization effect, and strong pertinence

Active Publication Date: 2014-10-15
SHANGHAI INNOSTAR BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims at the current lack of a guinea pig airway allergic asthma model, which is a clinically common disease animal model, and provides a preparation method for a guinea pig airway allergic asthma model

Method used

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  • Preparation method of guinea pig airway allergic asthma model
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  • Preparation method of guinea pig airway allergic asthma model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Guinea Pig Airway Allergic Asthma Model

[0021] Experimental animals: 12 guinea pigs, half male and half male. Hartley-Dunkin, SPF grade, weighing 300-360 g, was purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0022] The experimental ovalbumin (OVA) was purchased from SIGMA.

[0023] The breeding environment is as follows: temperature: 20-26°C; humidity: 40%-70%; ventilation rate: not less than 15 ventilation times per hour, using 100% fresh air (no air circulation). Lighting: Automatic lighting, alternating light and dark every 12 hours, turn off the lights at 7:00PM, and turn on the lights at 7:00AM the next day.

[0024] The guinea pig airway allergic asthma model preparation method is mainly divided into two stages: the sensitization period and the challenge period.

[0025] The sensitization period was: guinea pigs were sensitized by intraperitoneal injection of 3 mg / ml OVA normal saline solution 3 ml / kg,...

Embodiment 2

[0044] Example 2 Preparation of Guinea Pig Airway Allergic Asthma Model

[0045] Please refer to the technical content described in Example 1 for the preparation method and feeding environment of the experimental animals. The guinea pig airway allergic asthma model preparation method is mainly divided into two stages: the sensitization period and the challenge period. The sensitization period was as follows: guinea pigs were sensitized by intraperitoneal injection of 2 mg / ml OVA normal saline solution 2 ml / kg, once every other day, and the administration time was D1, D3, D5 and D7 days, a total of 4 administrations. The challenge period is: 12 days after the last sensitization (D19), challenge with a single nebulized administration. Put the guinea pig into the animal test tube of the IN-TOX small animal nasal exposure system, and insert the animal test tube into the exposure chamber. Inhale the atomized OVA physiological saline solution (its concentration is 4mg / ml) to attac...

Embodiment 3

[0046] Example 3 Preparation of Guinea Pig Airway Allergic Asthma Model

[0047] Please refer to the technical content described in Example 1 for the preparation method and feeding environment of the experimental animals. The guinea pig airway allergic asthma model preparation method is mainly divided into two stages: the sensitization period and the challenge period. The sensitization period was: guinea pigs were sensitized by intraperitoneal injection of 5 mg / ml OVA saline solution 5 ml / kg, once every other day, and the administration time was D1 and D3, and the administration was administered twice in total. The challenge period is: 15 days after the last sensitization (D18), challenge with a single nebulized administration. Put the guinea pig into the animal test tube of the IN-TOX small animal nasal exposure system, and insert the animal test tube into the exposure chamber. Inhale the atomized OVA physiological saline solution (its concentration is 7mg / ml) to attack, se...

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Abstract

The invention discloses a preparation method of a guinea pig airway allergic asthma model. The preparation method comprises the steps as follows: (1) injecting an OVA (ovalbumin) normal saline solution into a guinea pig abdominal cavity once every other day, and injecting the solution two to four times totally to enable a guinea pig to be allergic; (2) on 12th-15th days after the last sensitization in Step (1), enabling the guinea pig to inhale the atomized OVA normal saline solution through the mouth and the nose so as to arouse airway allergic asthma. Compared with existing methods, the preparation method has the advantages that the aerosol inhalation delivery is simulated, an inhalation preparation for nasal delivery is simulated, the target organ is sole, the pertinence is high, and common guinea pig intravenous injection allergy arousing tests cannot predict whether the allergy is aroused due to inhalation of a drug through the respiratory tract or not. Experiments prove that the preparation method is good in allergic effect, the OVA normal saline solution which is only inhaled and atomized through the mouth and the nose serves as a way to arouse the allergy, and the guinea pig airway allergic asthma model is successfully established.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of a guinea pig airway allergic asthma model. Background technique [0002] At present, with the deepening understanding of lung diseases and respiratory function, the scope of application of nasal drug delivery has gradually expanded, and various inhalation preparations have been paid more and more attention as effective treatment methods. Quantitative inhalation aerosol has become the most effective maintenance treatment for asthma and chronic obstructive pulmonary disease because of its targeted and local delivery characteristics. Asthma itself is allergic and sensitive to the respiratory tract, it is very important to judge whether inhaled drugs are allergic to the patient. [0003] Usually low-dose ovalbumin (OVA) is used to construct a guinea pig allergic asthma model. In the sensitization stage: the asthma model group is sensitized by intraperit...

Claims

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Application Information

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IPC IPC(8): A61K38/38
Inventor 马璟李华胡天羽史民华刘毅黄欢夏南雅萍
Owner SHANGHAI INNOSTAR BIO TECH
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