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A kind of polysaccharide-dopamine composite biological glue and its application

A technology of dopamine and biological glue, applied in the field of biological glue, can solve problems such as poor adhesion ability, and achieve the effects of reducing loss, increasing tissue viscosity, and increasing surface viscosity

Active Publication Date: 2016-03-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a polysaccharide-dopamine composite bioglue and its application in repairing superficial cartilage defects, specifically to successfully modify dopamine on polysaccharide molecules through chemical synthesis to prepare a new type of polysaccharide-dopamine composite bioglue , using the oxidative self-polymerization properties of dopamine to form a layer of polysaccharide-dopamine coating on the surface of cartilage to improve the poor adhesion of cells and bioactive molecules on the surface of cartilage, while maintaining the original structure of the cartilage surface, which can effectively improve superficial cartilage. defect repair ability

Method used

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  • A kind of polysaccharide-dopamine composite biological glue and its application
  • A kind of polysaccharide-dopamine composite biological glue and its application
  • A kind of polysaccharide-dopamine composite biological glue and its application

Examples

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Embodiment 1

[0032] Embodiment 1: chondroitin sulfate-dopamine composite biological glue and its application

[0033] (1) Preparation of chondroitin sulfate-dopamine composite biological glue: in a nitrogen environment, add 200 mg of chondroitin sulfate sodium salt (ie, chondroitin sulfate, purchased from sigma) to 40 mL of MES buffer (0.1M, pH5.5), and stir until completely dissolved to make a polysaccharide solution. In a nitrogen environment, dissolve 218mgEDC and 247mgsulfo-NHS in 1mL MES buffer (0.1M, pH5.5), and after complete dissolution, make an EDC mixture; then add the EDC mixture to the polysaccharide solution in a nitrogen environment, The reaction was stirred at 25 °C for 30 min. 216.2 mg of dopamine was dissolved in 1 mL of MES buffer (0.1 M, pH 5.5), added to the above reaction solution, and reacted for 120 min at 25° C. under nitrogen. The product after the reaction was taken out, placed in a dialysis bag (MWCO3500), and dialyzed in distilled water for 3 days; finally, th...

Embodiment 2

[0042] Embodiment 2: Chondroitin sulfate-dopamine bioglue

[0043] (1) Preparation of chondroitin sulfate-dopamine bioglue: 200 mg of chondroitin sulfate was added to 40 mL of MES buffer (0.1 M, pH 5.9), and stirred in a nitrogen atmosphere until completely dissolved to prepare a polysaccharide solution. Dissolve 218mg of EDC and 247mg of sulfo-NHS in 1mL of MES buffer (0.1M, pH 5.9). After complete dissolution, the EDC mixture was obtained, added to the polysaccharide solution, and reacted for 30min at room temperature under nitrogen. 216.2 mg of dopamine was dissolved in 1 mL of MES buffer, added to the above reaction solution, and reacted for 60 min at room temperature (25° C.) under nitrogen. The reacted product was taken out, placed in a dialysis bag (MWCO3500), and dialyzed in distilled water for 3 days; finally, the product was freeze-dried (48 hours, 10Pa, -108°C) to obtain chondroitin sulfate-dopamine bio-jelly 192mg, And stored at 4°C protected from light.

[0044]...

Embodiment 3

[0046] Embodiment 3: sodium hyaluronate-dopamine bioglue

[0047] (1) Preparation of sodium hyaluronate-dopamine bioglue: 400 mg of sodium hyaluronate was added to 80 mL of MES buffer (0.1 M, pH 5.9), and stirred in a nitrogen atmosphere until completely dissolved to prepare a polysaccharide solution. Dissolve 437mg of EDC and 495mg of sulfo-NHS in 2mL of MES buffer (0.1M, pH 5.9). After complete dissolution, the EDC mixture was obtained, added to the polysaccharide solution, and reacted for 30min at room temperature (25°C) under nitrogen. 432 mg of dopamine was dissolved in 2 mL of MES buffer (0.1 M, pH 5.9), added to the above reaction solution, and reacted for 60 min at room temperature (25° C.) under nitrogen. The reacted product was taken out, placed in a dialysis bag (MWCO8000~14000), and dialyzed in distilled water for 3 days; finally, the product was freeze-dried (72 hours, 10Pa, -108°C) to obtain sodium hyaluronate-dopamine biogel 411mg, and stored at 4°C in the dark...

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Abstract

The invention discloses a polysaccharide-dopamine composite biogel and application of the biogel. A preparation method of the biogel comprises the following steps: dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide sodium in an MES (Methyl Ester Sulfonate) buffer solution with a pH value being 5-6 in an inert gas atmosphere so as to prepare an EDC (ethylcarbodiimide) mixed solution, subsequently adding the EDC mixed solution into a polysaccharide solution for reacting at 20-30 DEG C for 0.5-1 hour, then adding a dopamine solution for further reacting at 20-30 DEG C for 1-4 hours, dialyzing a reactant by taking distilled water as a dialyzate, taking trapped fluid for freezing and drying to obtain the polysaccharide-dopamine composite biogel. The polysaccharide-dopamine composite biogel is simple and effective in modification method and capable of increasing the surface viscidity of the cartilage tissue, reducing loss of therapeutic drugs or implanted cells and facilitating the repairing of the cartilage injury.

Description

(1) Technical field [0001] The invention relates to a bioglue, in particular to the preparation of the bioglue which can directly adhere to the surface of damaged cartilage and promote the adhesion of other bioactive substances or cells, and its coating method on the cartilage surface. (2) Background technology [0002] Cartilage is composed of a special extracellular matrix produced by chondrocytes and has no blood supply. Its main components include hyaluronic acid, chondroitin sulfate, and type II collagen. However, chondrocytes are usually located at the junction of bone and cartilage, their number is limited and their ability to migrate is weak due to the limitation of extracellular matrix. These characteristics of cartilage make it difficult to heal after injury. In recent years, the "autologous chondrocyte transplantation" (ACI) technology has been developed, that is, the healthy chondrocytes extracted from the patient's body are cultured in vitro and expanded in lar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/08
Inventor 欧阳宏伟汪燕艳牟秦孙亨
Owner ZHEJIANG UNIV
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