Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Preparation method and application of reduced glutathione lipidosome

A glutathione lipid and reduced technology, applied in liposome delivery, tripeptide components, microcapsules, etc., can solve the problem of low liposome inclusion rate and achieve good targeting and physical stability High, easy-to-storage effect

Inactive Publication Date: 2014-08-13
李永吉
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problem of low inclusion rate of liposomes prepared by existing methods, thereby providing a method for preparing reduced glutathione liposomes and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of reduced glutathione lipidosome

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0017] Specific embodiment one: the preparation method of a kind of reduced glutathione liposome of the present embodiment is to carry out according to the following steps:

[0018] 1. Weigh 100 to 200 parts of soybean lecithin and 50 to 200 parts of fatty acid glycerides, and then dissolve them in 10 to 100 parts of absolute ethanol, and heat them in a water bath until the temperature is 50°C to 80°C. Prepare the oil phase;

[0019] 2. Weigh 500 to 1000 parts of reduced glutathione and 10 to 100 parts of surfactant, then dissolve them in 20 to 200 parts of distilled water, heat in a water bath to a temperature of 50 to 80 degrees, Prepare the aqueous phase;

[0020] 3. Mix the oil phase prepared in step 1 with the water phase prepared in step 2, then emulsify at a temperature of 50°C to 80°C for 1h to 2h, remove absolute ethanol by rotary evaporation under reduced pressure, and add 10 to 20 parts Distilled water, pre-cooled in ice bath for 1h to 3h at a temperature of -4°C ...

specific Embodiment approach 2

[0025] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the fatty acid glyceride in step 1 is tripalmitin, trilaurin or monostearin. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 1, the water bath is heated to a temperature of 65°C. Others are the same as in the first or second embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
polydispersity indexaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method and application of reduced glutathione lipidosome, relates to a preparation method and application of lipidosome, and aims at solving the technical problem of low inclusion rate of the lipidosome prepared by the existing method. The method comprises the following steps: 1, weighing soya bean lecithin and fatty acid glycerides, dissolving into absolute ethyl alcohol, and heating in a water bath to prepare an oil phase; 2, weighing reduced glutathione and a surfactant to be dissolved into distilled water, and heating in the water bath to prepare a water phase; 3, mixing the prepared oil phase with the water phase, emulsifying, carrying out vacuum rotary evaporation to remove absolute ethyl alcohol, adding distilled water, pre-cooling after ice-bath, and then carrying out freeze drying to obtain lipidosome powder. The reduced glutathione lipidosome disclosed by the invention is applied to intravenous injection, oral medication, transdermal drug delivery, and a drug delivery system for the lung and eyes. The encapsulation efficiency of the reduced glutathione lipidosome disclosed by the invention is 68.15%+ / -1.53%. The preparation method is applied to the field of preparation of the lipidosome.

Description

technical field [0001] The invention relates to a preparation method and application of liposome. Background technique [0002] Reduced glutathione (reduced glutathione, referred to as GSH) is a natural active peptide with important physiological functions. It has strong reducing properties and can scavenge free radicals and peroxides. However, the effective components of the reduced glutathione solution will be decomposed and oxidized after 2.5-3 hours, and the content will change, resulting in a decrease in the stability of the solution. [0003] The patent titled "Reduced Glutathione Percutaneous Absorption Preparation and Its Preparation Method" uses the bag-like space structure of cyclodextrin to include the sulfhydryl part of reduced glutathione to improve the stability of the preparation, and at the same time Utilizing that the binding ability of cyclodextrin and GSH is lower than that of reduced glutathione and skin, GSH is released from the clathrate according to t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K9/127A61K9/51A61K38/06A61P39/06
Inventor 李永吉郭玉岩
Owner 李永吉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com