Excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and construction method thereof

A technology of athletes and ice and snow, applied in the field of cDNA library and its construction, can solve problems that are not suitable for extensive and in-depth scientific research, and achieve the effect of high titer and high recombination rate

Inactive Publication Date: 2014-05-21
HARBIN INST OF PHYSICAL EDUCATION
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AI Technical Summary

Problems solved by technology

[0004] Since blood, tissue samples and genomic DNA are genetic research materials that cannot be replicated and are not suitable for ext...

Method used

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  • Excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and construction method thereof
  • Excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and construction method thereof
  • Excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0050] 1. Main equipment

[0051] Electric pipette (Germany); -70 ℃ low temperature refrigerator (USA); PCR amplification instrument (Biomtera, Germany); ND-1000 nucleic acid protein detector (USA); Pall ultrapure water device (Pall, Germany); Sigmal- 15K low temperature centrifuge (Germany); automatic gel imaging analysis system (domestic); constant temperature culture oscillator (Shanghai); DHG-9203A electric constant temperature blast drying oven (Shanghai); PH030A incubator (Shanghai); HHS -21-4 water bath (Shanghai); electrophoresis apparatus and electrophoresis tank (Beijing Liuyi Factory)

[0052] 2. Main reagents

[0053] SMART TM cDNA Library Construction Kit (Clontech); Trizol (Invitrogen); Gigapack III Gold-7 (Stratagene). Other reagents are commonly used reagents in molecular biology laboratories.

[0054] 3. Extraction of total RNA

[0055] (1) Take the blood sample of an excellent ice and snow athlete, put it into a sodium citrate anticoagulant tube, turn it ...

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Abstract

The invention relates to an excellent skiing sportsman enriched cDNA (complementary Deoxyribonucleic Acid) library and a construction method thereof. A high-quality excellent skiing sportsman enriched cDNA library with high titer, high recombination rate and large insertion fragment range is obtained by performing total RNA (Ribonucleic Acid) extraction, double-chain cDNA synthesis, digestion using protease K, Sfi1 enzyme digestion, connection into a vector and package of lambda phage. The invention belongs to the field of interdiscipline of sports science, human resource methodology, cell biology and molecular biology.

Description

technical field [0001] The present invention relates to a cDNA library and its construction method. Specifically, the blood samples of several excellent ice and snow athletes are used to extract total RNA, mix total RNA, synthesize double-stranded cDNA, digest with proteinase K, digest with Sfi1 and connect to a carrier, The packaging of λ phage finally obtained the enriched cDNA library of excellent ice and snow athletes. Background technique [0002] Human genetic resources are irreplaceable resources for human beings to conduct their own exploration and research. Human genetic resources refer to the genetic material representing human genes. In today's world, the people who can best represent human's own physical fitness genes are excellent athletes in various sports. As a part of human genetic resources, sports genetic resources are precious wealth of human beings. However, due to the limitation that sports genetic resources only exist in individual athletes, if we do n...

Claims

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Application Information

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IPC IPC(8): C40B40/08C40B50/06C12Q1/68
Inventor 高俊江李兴洋高凡陆涛峰金红关伟军
Owner HARBIN INST OF PHYSICAL EDUCATION
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